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PEDOT:PSS microelectrode arrays for hippocampal cell culture electrophysiological recordings

  • Dimitrios A. Koutsouras (a1), Adel Hama (a1), Jolien Pas (a1), Paschalis Gkoupidenis (a1), Bruno Hivert (a2) (a3), Catherine Faivre-Sarrailh (a2) (a3), Eric Di Pasquale (a2) (a3), Róisín M. Owens (a1) and George G. Malliaras (a1)...

Abstract

In vitro electrophysiology using microelectrode arrays (MEAs) plays an important role in understanding fundamental biologic processes, screening potential drugs and assessing the toxicity of chemicals. Low electrode impedance and ability to sustain viable cultures are the key technology requirements. We show that MEAs consisting of poly(3,4-ethylenedioxythiophene) doped with poly(styrene sulfonate) (PEDOT:PSS) and coated with poly-L-lysine satisfy these requirements. Hippocampal cell cultures, maintained for 3–6 weeks on these MEAs, give high quality recordings of neural activity. This enables the observation of drug-induced activity changes, which paves the way for using these devices in in vitro drug screening and toxicology applications.

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Corresponding author

Address all Correspondence to George G. Malliaras at malliaras@emse.fr

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This author was an editor of this journal during the review and decision stage. For the MRC policy on review and publication of manuscripts authored by editors, please refer to http://www.mrs.org/editor-manuscripts/.

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1. Pine, J.: Recording action potentials from cultured neurons with extracellular microcircuit electrodes. J. Neurosci. Methods 2, 19 (1980).
2. Nam, Y. and Wheeler, B.C.: In vitro microelectrode array technology and neural recordings. Crit. Rev. Biomed. Eng. 39, 45 (2011).
3. Steidl, E.M., Neveu, E., Bertrand, D., and Buisson, B.: The adult rat hippocampal slice revisited with multi-electrode arrays. Brain Res. 1096, 70 (2006).
4. Sessolo, M., Khodagholy, D., Rivnay, J., Maddalena, F., Gleyzes, M., Steidl, E., Buisson, B., and Malliaras, G.G.: Easy-to-Fabricate conducting polymer microelectrode arrays. Adv. Mater. 25, 2135 (2013).
5. Arnold, F.J., Hofmann, F., Bengtson, C.P., Wittmann, M., Vanhoutte, P., and Bading, H.: Microelectrode array recordings of cultured hippocampal networks reveal a simple model for transcription and protein synthesis-dependent plasticity. J. Physiol. 564, 3 (2005).
6. Kanagasabapathi, T.T., Massobrio, P., Barone, R.A., Tedesco, M., Martinoia, S., Wadman, W.J., and Decre, M.M.: Functional connectivity and dynamics of cortical-thalamic networks co-cultured in a dual compartment device. J Neural Eng 9, 036010 (2012).
7. Soldatow, V.Y., LeCluyse, E.L., Griffith, L.G., and Rusyn, I.: In vitro models for liver toxicity testing. Toxicol. Res. 2, 23 (2013).
8. Rivnay, J., Owens, R.M., and Malliaras, G.G.: The rise of organic bioelectronics. Chem. Mater. 26, 679 (2014).
9. Buzsaki, G., Anastassiou, C.A., and Koch, C.: The origin of extracellular fields and currents–EEG, ECoG, LFP and spikes. Nat. Rev. Neurosci. 13, 407 (2012).
10. Ludwig, K.A., Langhals, N.B., Joseph, M.D., Richardson-Burns, S.M., Hendricks, J.L., and Kipke, D.R.: Poly(3,4-ethylenedioxythiophene) (PEDOT) polymer coatings facilitate smaller neural recording electrodes. J. Neural. Eng. 8, 014001 (2011).
11. Nam, Y., Wheeler, B.C., and Heuschkel, M.O.: Neural recording and stimulation of dissociated hippocampal cultures using microfabricated three-dimensional tip electrode array. J. Neurosci. Methods 155, 296 (2006).
12. Geissler, M. and Faissner, A.: A new indirect co-culture set up of mouse hippocampal neurons and cortical astrocytes on microelectrode arrays. J. Neurosci. Methods 204, 262 (2012).
13. Yang, Z., Zhao, Q., Keefer, E., and Liu, W.: Noise Characterization, Modeling, and Reduction for In Vivo Neural Recording, edited by Bengio, Y., Schuurmans, D., Lafferty, J., Williams, C.K.I. and Culotta, A. (NIPs Proc. 22, Vancouver, BC, Canada, 2009), p. 2160.
14. Ludwig, K.A., Uram, J.D., Yang, J., Martin, D.C., and Kipke, D.R.: Chronic neural recordings using silicon microelectrode arrays electrochemically deposited with a poly(3,4-ethylenedioxythiophene) (PEDOT) film. J. Neural. Eng. 3, 59 (2006).
15. Kovacs, G.T.A.: Introduction to the theory, design and modeling of thin-film microelectrodes for neural interfaces. In Enabling Technologies for Cultured Neural Networks, edited by Stenger, D.A. and McKenna, T.M. (Academic Press, London, 1994), p. 121.
16. Berggren, M. and Richter-Dahlfors, A.: Organic bioelectronics. Adv. Mater. 19, 3201 (2007).
17. Green, R. and Abidian, M.R.: Conducting polymers for neural prosthetic and neural interface applications. Adv. Mater. 27, 7620 (2015).
18. Martin, D.C. and Malliaras, G.G.: Interfacing electronic and ionic charge transport in bioelectronics. ChemElectroChem 3, 686 (2016).
19. Proctor, C.M., Rivnay, J., and Malliaras, G.G.: Understanding volumetric capacitance in conducting polymers. J.Polym. Sci. Part B:Polym. Phys. 54, 1433 (2016).
20. Nyberg, T., Shimada, A., and Torimitsu, K.: Ion conducting polymer microelectrodes for interfacing with neural networks. J. Neurosci. Methods 160, 16 (2007).
21. Blau, A., Murr, A., Wolff, S., Sernagor, E., Medini, P., Iurilli, G., Ziegler, C., and Benfenati, F.: Flexible, all-polymer microelectrode arrays for the capture of cardiac and neuronal signals. Biomaterials 32, 1778 (2011).
22. Richardson-Burns, S.M., Hendricks, J.L., Foster, B., Povlich, L.K., Kim, D.-H., and Martin, D.C.: Polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) around living neural cells. Biomaterials 28, 1539 (2007).
23. Cui, X., Lee, V.A., Raphael, Y., Wiler, J.A., Hetke, J.F., Anderson, D.J., and Martin, D.C.: Surface modification of neural recording electrodes with conducting polymer/biomolecule blends. J.Biomed. Mater. Res. 56, 261 (2001).
24. Richardson, R.T., Thompson, B., Moulton, S., Newbold, C., Lum, M.G., Cameron, A., Wallace, G., Kapsa, R., Clark, G., and O'Leary, S.: The effect of polypyrrole with incorporated neurotrophin-3 on the promotion of neurite outgrowth from auditory neurons. Biomaterials 28, 513 (2007).
25. Green, R.A., Hassarati, R.T., Bouchinet, L., Lee, C.S., Cheong, G.L., Yu, J.F., Dodds, C.W., Suaning, G.J., Poole-Warren, L.A., and Lovell, N.H.: Substrate dependent stability of conducting polymer coatings on medical electrodes. Biomaterials 33, 5875 (2012).
26. Abidian, M.R., Corey, J.M., Kipke, D.R., and Martin, D.C.: Conducting-polymer nanotubes improve electrical properties, mechanical adhesion, neural attachment, and neurite outgrowth of neural electrodes. Small 6, 421 (2010).
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