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Diamond microelectrode arrays for in vitro neuronal recordings

Published online by Cambridge University Press:  09 August 2017

Matthew McDonald ,
Antonina Monaco ,
Farnoosh Vahidpour ,
Ken Haenen ,
Michele Giugliano  and
Milos Nesladek
Matthew McDonald*
Affiliation:
Institute of Materials Research, Hasselt University, 3590 Diepenbeek, Belgium
Antonina Monaco*
Affiliation:
Department of Biomedical Sciences, University of Antwerp, B-2610 Wilrijk, Belgium
Farnoosh Vahidpour
Affiliation:
Institute of Materials Research, Hasselt University, 3590 Diepenbeek, Belgium
Ken Haenen
Affiliation:
Institute of Materials Research, Hasselt University, 3590 Diepenbeek, Belgium
Michele Giugliano
Affiliation:
Department of Biomedical Sciences, University of Antwerp, B-2610 Wilrijk, Belgium Brain Mind Institute, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland Department of Computer Science, University of Sheffield, Sheffield S1 4DP, UK
Milos Nesladek
Affiliation:
Institute of Materials Research, Hasselt University, 3590 Diepenbeek, Belgium Interuniversity Microelectronics Center (IMEC), B-3001 Heverlee, Belgium
*
Address all correspondence to M. McDonald at mattmcd87@gmail.com and A. Monaco at antonina.monaco@uantwerpen.be
Address all correspondence to M. McDonald at mattmcd87@gmail.com and A. Monaco at antonina.monaco@uantwerpen.be
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Abstract

A novel microfabrication technique for microelectrode arrays (MEAs) with a full diamond–cell interface is demonstrated. Boron-doped nano-crystalline diamond (BNCD) is used as a conductive electrode material on metal tracks insulated by intrinsic NCD. MEAs successfully recorded spontaneous electrical activity in rat primary cortical neuronal cultures. Patch-clamp measurements show no alterations to cell membrane passive properties or active firing response, for cell developing ex vivo on diamond. Impedance analysis revealed low impedance magnitude of BNCD electrodes, suitable for multi-unit neuronal recordings. Additionally, the impedance phase of the fabricated electrodes shows a high degree of capacitive coupling, ideal for neuron stimulation.

Type
Research Letters
Information
MRS Communications , Volume 7 , Issue 3 , September 2017 , pp. 683 - 690
Copyright
Copyright © Materials Research Society 2017 

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