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Transparent carbon nanotube electrodes for electric cell–substrate impedance sensing

Published online by Cambridge University Press:  30 August 2019

Shokoufeh Teymouri*
Affiliation:
Department of Electrical and Computer Engineering, Institute for Nanoelectronics, Technical University of Munich, Arcisstraße 21, 80333Munich, Germany ibidi GmbH, Lochhamer Schlag 11, 82166Gräfelfing, Germany
Florin Loghin
Affiliation:
Department of Electrical and Computer Engineering, Institute for Nanoelectronics, Technical University of Munich, Arcisstraße 21, 80333Munich, Germany
Marco Bobinger
Affiliation:
Department of Electrical and Computer Engineering, Institute for Nanoelectronics, Technical University of Munich, Arcisstraße 21, 80333Munich, Germany
Zeno Guttenberg
Affiliation:
ibidi GmbH, Lochhamer Schlag 11, 82166Gräfelfing, Germany
Paolo Lugli
Affiliation:
Faculty of Science and Technology, Free University of Bozen-Bolzano, 39100Bolzano, Italy
*
Address all correspondence to Shokoufeh Teymouri at shteymouri@ibidi.de
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Abstract

Electric cell–substrate impedance sensing is widely used to study cell behavior such as adhesion, migration, and cell toxicity. However, a simultaneous optical imaging of cells is limited by inefficient transmission of visible light through the gold electrodes. To overcome this limitation, we fabricated carbon nanotube (CNT) electrodes with high electrical conductivity as well as optical transmittance. The impedimetric monitoring of cell proliferation and migration by gold and CNT electrodes were compared and analyzed. Taking advantage of the optical transparency of CNTs, we demonstrated a simultaneous electronic and optical monitoring of MCF7 cells, with acquisition of high-resolution images.

Type
Research Letters
Copyright
Copyright © The Author(s) 2019

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