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Electrospun Fibers for Controlled Release of Nanoparticle-Assisted Phage Therapy Treatment of Topical Wounds

  • Jessica M. Andriolo (a1) (a2), Nathan J. Sutton (a1) (a2), John P. Murphy (a2), Lane G. Huston (a1) (a2), Emily A. Kooistra-Manning (a2), Robert F. West (a2), Marisa L. Pedulla (a3), M. Katie Hailer (a2) (a4) and Jack L. Skinner (a1) (a2)...

Abstract

Bacterial cultures exposed to iron-doped apatite nanoparticles (IDANPs) prior to the introduction of antagonistic viruses experience up to 2.3 times the bacterial destruction observed in control cultures. Maximum antibacterial activity of these bacteria-specific viruses, or phage, occurs after bacterial cultures have been exposed to IDANPs for 1 hr prior to phage introduction, demonstrating that IDANP-assisted phage therapy would not be straight forward, but would instead require controlled time release of IDANPs and phage. These findings motivated the design of an electrospun nanofiber mesh treatment delivery system that allows burst release of IDANPs, followed by slow, consistent release of phage for treatment of topical bacterial infections. IDANPs resemble hydroxyapatite, a biocompatible mineral analogous to the inorganic constituent of mammalian bone, which has been approved by the Food and Drug Administration for many biomedical purposes. The composite nanofiber mesh was designed for IDANP-assisted phage therapy treatment of topical wounds and consists of a superficial, rapid release layer of polyethylene oxide (PEO) fibers doped with IDANPs, followed by inner, coaxial polycaprolactone / polyethylene glycol (PCL/PEG) blended polymer fiber layer for slower phage delivery. Our investigations have established that IDANP-doped PEO fibers are effective vehicles for dissemination of IDANPs for bacterial exposure and resultant increased bacterial death by phage. In this work, slower delivery of the phage behind IDANPs was accomplished using coaxial, electrospun fibers composed of PCL/PEG polymer blend.

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[1]Antibiotic Resistance Threats in the United States 2013, (U.S. Department of Health and Human Services, Centers for Disease Control and Prevention 2013), pp. 11–12.
[2]d’Herelle, F., B. New York Acad. Med. 7, 329 (1931).
[3]Haq, I. U., Chaudhry, W. N., Akhtar, M. N., Andleeb, S., and Qadri, I., Virol. J. 9, (2012).
[4]Chhibber, S., Kaur, T., and Kaur, S., PloS One 8, (2013).
[5]Mendes, J. J., Leandro, C., Corte-Real, S., Barbosa, R., Cavaco-Silva, P., Melo-Cristino, J., Gorski, A., and Garcia, M., Wound Repair Regener. 21, 595 (2013).
[6]Lungren, M. P., Christensen, D., Kankotia, R., Falk, I., Paxton, B. E., and Kim, C. Y., Bacteriophage 3, (2013).
[7]Yilmaz, C., Colak, M., Yilmaz, B. C., Ersoz, G., Kutateladze, M., and Gozlugol, M., J. Bone Jt. 95, 117 (2013).
[8]Miᶒdzybrodzki, R., Fortuna, W., Weber-Dᶏbrowska, B., and Górski, A., Postepy. Hig. Med. Dosw. 61, 461 (2007).
[9]Parasion, S., Kwiatek, M., Gryko, R., Mizak, L., Malm, A., Pol. J. Microbiol. 63, 137 (2014).
[10]Doss, J., Culbertson, K., Hahn, D., Camacho, J. and Barekzi, N., Viruses 9, (2017).
[11]Andriolo, J.M., Hensleigh, R.M., McConnell, C.A., Pedulla, M., Hailer, K., Kasinath, R., Wyss, G., Gleason, W., and Skinner, J.L., J. Vac. Sci. Technol. B 32, (2014).
[12]Andriolo, J. M., Rossi, R. J., McConnell, C. A., Connors, B. I., Trout, K. L., Hailer, M. K., and Skinner, J. L., J. Vac. Sci. Technol. 15, 908 (2016).
[13]Palmer, L. C., Newcomb, C. J., Kaltz, S. R., Spoerke, E. D., and Stupp, S. I., Chem. Rev. 108, 4754 (2008).
[14]Šupová, M., Ceram. Int. 41, 9203 (2015).
[15]Prem, V. S. and Chandra, S., J. Biomater Tissue Eng. 2, 269 (2012).
[16]Sahdev, P., Podaralla, S., Kaushik, R. S., and Perumal, O., J. Biomed. Nanotechnol. 9, 132 (2013).
[17]Lee, D., Upadhye, K., and Kumta, P.N., Mater. Sci. Eng. B 177, 269 (2012).
[18]Keshri, A. K. and Agarwal, A., Nanosci. Nanotechnol. Let. 4, 228 (2012).
[19]Ezhaveni, S., Yuvakkumar, R., Rajkumar, M., Sundaram, N. M., and Rajendran, V., J. Nanosci. Nanotechnol. 13, 1631 (2013).
[20]Andriolo, J. M., Wyss, G. F., Murphy, J. P., Pedulla, M. L., Hailer, M. K., and Skinner, J. L., MRS Advances 2, 2465 (2017).
[21]Korehei, R. and Kadla, J. F., Carbohyd. Polym. 100, 150 (2014).
[22]Korehei, R. and Kadla, J., J. Appl. Microbiol. 114, 1425 (2013).
[23]Salalha, W., Kuhn, J., Dror, Y., and Zussman, E., Nanotechnology 17, 4675 (2006).
[24]Dalton, P.D., Klinkhammer, K., Salber, J., Klee, D., and Möller, M., Biomacromolecules 7, 686 (2006).
[25]Kim, J.S. and Lee, D.S., Polym. J. 32, 616 (2000).
[26]Larrondo, L. and Manley, R.S.J., J. Polym. Sci. Pol. Phys. 19, 515 (1981).
[27]Lee, S. and Obendorf, S.K., J. Appl. Polym. Sci. 102, 3430 (2006).
[28]Lyons, J. and Li, C., Ko, F., Polymer 45, 7597 (2004).
[29]Fister, S., Robben, C., Witte, A. K., Schoder, D., Wagner, M., and Rossmanith, P., Front. Microbiol. 7, 1152 (2016).
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MRS Advances
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