Skip to main content Accessibility help
×
Home

Compared Biocompatibility of ZnTiO3, ZnO and TiO2 Sol-Gel Films with Human Mesenchymal Stem Cells

  • Rosalía Delgado Carrascón (a1), Dario Gallach Pérez (a1), Josefa.P. Carcía Ruiz (a2) and Miguel Manso Silvan (a1)

Abstract

The biomedical applications of ZnO are drastically limited by its intrinsic solubility, which shortens the stability and lifetime of devices. We show that the functionality of ZnO in human mesenchymal stem cell (hMSC) studies is limited due to poor cell adhesion. The sol-gel route has been employed to obtain zinc titanate thin films for their integration as surface protective layer on ZnO. These films were obtained from zinc acetate (ZnAc) and titanium isopropoxide (TIPT). So derived xerogels were dried and their thermal evolution studied by TGM-DTA to identify critical annealing temperatures. The evolution of the microstructure and composition of spun cast films was determined by XRD and FTIR. Organic and ionic byproducts were eliminated at T>300°C, which kickstarts a transformation of the amorphous materials into polycrystalline. Thin films consisted of the ZnTiO3 perovskite from annealing temperatures of 500°C. Cell adhesion on the synthesized samples (both amorphous and crystalline) was assayed by culturing hMSCs. Immunofluorescence images of actin cytoskeleton were obtained and proliferation studied using Ki67. Cell density, single cell area and proliferation rates on ZnTiO3 films were closer to control TiO2 surfaces than to ZnO films. Such behavior validates the short term biocompatibility of ZnTiO3 films and its potential use as surface layer for ZnO biomedical devices.

Copyright

Corresponding author

References

Hide All
1.Krylova, G., Brioude, A., Ababou-Girard, S., Mrazek, J., Spanhel, L., Phys. Chem. Chem. Phys. 12 15101 (2010).
2.Tangi, A., Grasset, F., Potel, M., Nazabal, V., Cardinal, T., Pechev, S., Saito, N., Ohashi, N., Haneda, H.. Sci. Technol. Adv. Mater. 11 044401 (2010).
3.Wu, S.C., Yau, W.H., Tsai, C.H., Chou, C.P.. Surf. Interf. Anal. 44 1314 (2012).
4.Manso, M., Ogueta, S., García, P., Pérez-Regueiro, J., Jiménez, C., Martínez-Duart, J.M., Langlet, M.. Biomaterials 23 349 (2002).
5.Gupta, R., Kumar, A.. Biomed. Mater. 3 034005 (2008).
6.Wang, D., Bierwagen, G.P.. Progr. Org. Coat. 64 327 (2009).
7.Znaidi, L.. Mater. Sci. Eng. B 174 18 (2010).
8.Chang, Y.S., Chang, Y.H., Chen, I.G., Chen, G.J., Chai, Y.L.. J. Crys. Growth 243 319 (2002).
9.Mohammadi, M.R., Fray, D.J.. J. Eur. Cer. Soc. 30 947 (2010).
10.Yamaguchi, O., Morimi, M., Kawabata, H., Shimizu, K.. J. Am. Cer. Soc. 70 97 (1987).
11.Kumar, K., Chitkaraa, M., Sandhua, I.S., Mehta, D., Kumar, S.. J. All. Comp. 588 681 (2014).

Keywords

Compared Biocompatibility of ZnTiO3, ZnO and TiO2 Sol-Gel Films with Human Mesenchymal Stem Cells

  • Rosalía Delgado Carrascón (a1), Dario Gallach Pérez (a1), Josefa.P. Carcía Ruiz (a2) and Miguel Manso Silvan (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed