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Kinetics for De-Extracellular Matrix(ECM)

Published online by Cambridge University Press:  11 May 2018

Namsoo P. Kim
Affiliation:
Metallurgical, Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA Printing Nano Engineering Lab, Metallurgical, Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
Jihye Kim
Affiliation:
Printing Nano Engineering Lab, Metallurgical, Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
Guikuan Yue
Affiliation:
Metallurgical, Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
Diana Cho
Affiliation:
Printing Nano Engineering Lab, Metallurgical, Materials and Biomedical Engineering Department, The University of Texas at El Paso, El Paso, TX, USA
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Abstract

A kinetic model for ECM decellularization with Trypsin and Deoxycholic Acid as reactants in a batch system has been developed. The decellularization mechanism has been analysed by solving a series of ordinary differential equations relating heterogeneous reaction kinetics at the solid/liquid interface to mass transfer of reactant and products. In order to remove the Deoxyribonucleic acid (DNA) of porcine, special emphasis has been given to the surface area for regulating the depth and amount of the penetration of the reagent, the numerical analysis thereof, and the corresponding experiment were carried out. The effect of chemical reaction order, rate constants and mass transfer coefficients on the overall decellularization rate has been analysed and discussed.

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Copyright
Copyright © Materials Research Society 2018 

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