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Microbial degradation of isosaccharinic acid under conditions representative for the far field of radioactive waste disposal facilities

Published online by Cambridge University Press:  02 January 2018

Gina Kuippers ,
Naji Milad Bassil ,
Christopher Boothman ,
Nicholas Bryan  and
Jonathan R. Lloyd
Gina Kuippers*
Affiliation:
Research Centre for Radwaste and Decommissioning & Williamson Research Centre for Molecular Environmental Science, School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
Naji Milad Bassil
Affiliation:
Research Centre for Radwaste and Decommissioning & Williamson Research Centre for Molecular Environmental Science, School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
Christopher Boothman
Affiliation:
Research Centre for Radwaste and Decommissioning & Williamson Research Centre for Molecular Environmental Science, School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
Nicholas Bryan
Affiliation:
National Nuclear Laboratory, Chadwick House, Birchwood, Warrington WA3 6AE, UK
Jonathan R. Lloyd
Affiliation:
Research Centre for Radwaste and Decommissioning & Williamson Research Centre for Molecular Environmental Science, School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
*
*E-mail: gina.kuippers@manchester.ac.uk
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Abstract

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It is UK Government policy to dispose of higher activity radioactive waste through geological disposal into an engineered deep underground geological disposal facility (GDF; DECC, 2014). Those wastes include low-level (LLW) and intermediate-level (ILW) radioactive wastes that are very heterogeneous, containing a range of inorganic and organic materials, the latter including cellulosic items. After closure of the GDF, eventual resaturation with groundwater is expected, resulting in the development of a hyperalkaline environment due to the proposed use of a cementitious backfill. Under these high-pH conditions, cellulose is unstable and will be degraded chemically, forming a range of water-soluble, low molecular weight compounds, of which the most abundant is isosaccharinic acid (ISA). As ISA is known to form stable soluble complexes with a range of radionuclides, thereby increasing the chance of radionuclide transport, the impact of microbial metabolism on this organic substrate was investigated to help determine the role of microorganisms in moderating the transport of radionuclides from a cementitious GDF. Anaerobic biodegradation of ISA has been studied recently in high-pH cementitious ILW systems, but less work has been done under anaerobic conditions at circumneutral conditions, more representative of the geosphere surrounding a GDF. Here we report the fate of ISA in circumneutral microcosms poised under aerobic and anaerobic conditions; the latter with nitrate, Fe(III) or sulfate added as electron acceptors. Data are presented confirming the metabolism of ISA under these conditions, including the direct oxidation of ISA under aerobic and nitrate-reducing conditions and the fermentation of ISA to acetate, propionate and butyrate prior to utilization of these acids during Fe(III) and sulfate reduction. The microbial communities associated with these processes were characterized using 16S rRNA gene pyrosequencing. Methane production was also quantified in these experiments, and the added electron acceptors were shown to play a significant role in minimizing methanogenesis from ISA and its breakdown products.

Keywords

isosaccharinic acidcomplexradionuclide mobilitybiodegradationnuclear wastegeological disposal
Type
Research Article
Information
Mineralogical Magazine , Volume 79 , Issue 6 , November 2015 , pp. 1443 - 1454
Creative Commons
Creative Common License - CCCreative Common License - BY
Copyright © The Mineralogical Society of Great Britain and Ireland 2015. This is an open access article, distributed under the terms of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
Copyright © The Mineralogical Society of Great Britain and Ireland 2015

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