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Reconstructing What Was: Software Applied To Serial Section TEM

  • Marcia D. Feinberg (a1) and John C. Fiala (a1)

Extract

The well-known recipe for serial reconstruction in microscopy consists of the following six steps: fix and embed specimen, cut and mount sections for microscopy, image sections, align images in the computer, identify and trace profiles in images, generate three-dimensional (3D) visualizations. The free software program Reconstruct facilitates the final steps of this process for many types of microscopy, including scanning electron or ion beam microscopy applied to material and light microscopy applied to biological samples. We describe here how serial sectioning and transmission electron microscopy (TEM) can be combined with the Reconstruct software to generate large volumes of soft tissue ultrastructure for analysis.

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References

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2 Bozzola, JJ, Russell, LD (1998) Electron Microscopy. Principles and Techniques for Biologists, Second Edition. Jones and Bartlett Publishers: Boston.
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10 Hayat, MA (2000) Principles and Techniques of Electron Microscopy. Biological Applications, 4th edition, Cambridge University Press.
11 Hoffpauir, BK, Pope, BA, Spirou, GA (2007) Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held. Nature Protocols 2(1):922.

Reconstructing What Was: Software Applied To Serial Section TEM

  • Marcia D. Feinberg (a1) and John C. Fiala (a1)

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