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Programmable Array Microscopes

Published online by Cambridge University Press:  14 March 2018

Quentin Hanley ,
Rainer Heintzmann ,
Donna Arndt-Jovin  and
Thomas Jovin
Quentin Hanley*
Affiliation:
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Rainer Heintzmann
Affiliation:
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Donna Arndt-Jovin
Affiliation:
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Thomas Jovin*
Affiliation:
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
*
qhanley or tjovin @mpc186.mpibpc.gwdg.de.
qhanley or tjovin @mpc186.mpibpc.gwdg.de.

Extract

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The programmable array microscope (PAM) is a powerful tool combining the capabilities of nearly all previously described optical sectioning techniques in a single microscope. Not only can the user create optical sections of threedimensional objects, but the PAM's unique adaptive optical strategy allows a user to select the best sectioning method for a particular sample or experimental need. The key to the PAM is a spatial light modulator (SLM). This device, when placed in the image plane of a microscope, can be used to create optical sectioning, generate spatial encoding masks, and/or define regions of interest.

Type
Research Article
Information
Microscopy Today , Volume 9 , Issue 2 , March 2001 , pp. 8 - 13
Copyright
Copyright © Microscopy Society of America 2001

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