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Applications of Focused Ion Beam (FIB) on Yeast Cell & SARS Virus

  • H. L. Hing (a1), C. Burkhardt (a2), P. Gnauck (a2), S. Sally (a3), H. Gelderbloms (a4), Y. Muranaka (a5), M.A. Kaswandi (a1), A.H.A. Azizl (a1) and A.Z. Sahalan (a1)...

Extract

The focused ion beam (FIB) is a relatively novel technique to biomedical electron microscopy as it open up new means for the observations and examinations of a wide range of biomedical and biological materials. The focused ion beam, or FIB tool has been utilized mainly in the fields of material sciences and industry. The (FIB) uses high-energy gallium ions to precisely and accurately section or mill samples. Lately FIB method have been used to prepare biological samples such as yeast cells and virus particles. Yeast cells Schwanniomyces occidentalis S. occidentalis were prepared by vacuum sucking them into cellulose tubing, plunge freezing them in liquid nitrogen, followed by chemical fixation in glutaraldehye and postfixed with osmium tetroxide, dehydrated in a series of ascending alcohol concentration up to absolute alcohol, then freeze dried overnight. In the case of SARS virus, the tissue culture containing virus particles was chemically fixed with glutaraldehyde, dehydrated in ascending order of alcohol concentrations and then freeze dried.

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References

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[1]. Monroe, P.R. & Dickson, M.A. 2001. Proc. of the 10th Scientific Conference Electron Microscopy Soc of Malaysia, Kuala Lumpur, Malaysia (2001): 8.
[2]. Hing, H.L., Muranaka, Y., Normalawati, S., Kaswandi, M.A., Aziz, A.H. Abd., Aoshima, A.Z. Sahalan & Tsutsui, Y.. J. Electron Microscopy Soc Thailand (2004) 17: 5.
[3]. Hing, H.L., Burkhardt, C., Gnauck, P., Sally, S., Gelderbloms, H., Muranaka, Y., Kaswandi, M.A., Sahalan, A.Z. & M Yasin, M.S.. J. Electron Microscopy Soc. Thailand (2006) 19: 4.
[4]. The contributions Dr. Sally Stowe, EM Unit, ANU, Canberra, Australia & Dr. Peter Gnauck of Zeiss, Germany, Ms Normalawati Shamsudin is greatly acknowledged.

Applications of Focused Ion Beam (FIB) on Yeast Cell & SARS Virus

  • H. L. Hing (a1), C. Burkhardt (a2), P. Gnauck (a2), S. Sally (a3), H. Gelderbloms (a4), Y. Muranaka (a5), M.A. Kaswandi (a1), A.H.A. Azizl (a1) and A.Z. Sahalan (a1)...

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