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A Simple Method for the Cross-Section Area Determination of Single Profiled Fibers and Its Application

Published online by Cambridge University Press:  30 January 2018

Zhangchuan Peng ,
Chun Liu ,
Lin Zhang ,
Wei Li ,
Wenbo Hu ,
Sanyuan Ma  and
Qingyou Xia
Zhangchuan Peng
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Chun Liu*
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China
Lin Zhang
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Wei Li
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Wenbo Hu
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Sanyuan Ma
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Qingyou Xia
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China
*
Author for correspondence: Chun Liu, E-mail: mlliuchun@163.com
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Abstract

One of the critical prerequisites for accurately measuring the mechanical properties of profiled fibers is the precise determination of their cross-sectional areas (CSAs). In this study, a new method is established for determining a single profiled fibers’ CSA based on the frozen section method and digital photo, pixel-ratio method (FS-DP). FS-DP is used to obtain a transverse section of a fiber, by acquiring an image of the cross section using optical microscopy or scanning electron microscopy, and then calculating the CSA using Photoshop. Using FS-DP, it was found that the shape of a fiber of silk changes little in a range of 50 μm, but varies considerably over a range of 1 m, while the CSA of cocoon silk (900 m) first increases and then decreases. Mechanical property tests showed that the elongation, strength, elastic modulus, and toughness values of the cocoon silk are consistent with those reported previously. Additionally, FS-DP was also used to observe other profiled fibers. The application tests indicated that FS-DP can be used to quickly and accurately obtain the CSA of a single profiled fiber, and that it is suitable for the large-scale determination and analysis of the mechanical properties of profiled fibers.

Keywords

frozen section methodcross-sectional areaprofiled fibersilkworm silk fiberoptical microscope
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 24 , Issue 1 , February 2018 , pp. 17 - 28
Copyright
© Microscopy Society of America 2018 

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