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Short-Term Response of Mitomycin C on the Human Rectus Muscle Following Strabismus Surgery: Histological, Ultrastructural, and Biomechanical Evaluation

  • Samjin Choi (a1) (a2), Youjin Cheong (a1), Jae-Ho Shin (a3), Kyung A Kim (a2), Jae Beum Bang (a4), Kyung-Hyun Jin (a3) and Hun-Kuk Park (a1) (a5)...

Abstract

This study investigated the inflammatory effect of intraoperative mitomycin C (MMC) on adhesion reformation in human rectus muscles. Ten consecutive patients who underwent medial rectus resection had their postoperative rectus muscles divided into two groups: control group (n = 10) and MMC group (n = 10). In the MMC group, the muscle was soaked for 2 min with MMC, prepared as a 0.2 mg/mL (0.02%) solution. The 0.02% MMC reactions were examined using histological analysis with hematoxylin-eosin (inflammatory response) and Masson's trichrome (collagen fibrils), immunoreactivities of cyclooxygenase-II (inflammatory response), and collagen type I and III, scanning electron microscopy analysis to quantify the diameter and D-periodicity of collagen fibrils, and atomic force microscopy analysis to quantify the diameter, D-periodicity, and adhesion force of collagen fibrils. The rectus muscles treated with 0.02% MMC showed a significantly increased inflammatory response (p < 0.05), increased collagen density (p < 0.0001), increased fibril diameter (p < 0.001 or p < 0.05), and decreased fibril adhesion force (p < 0.005) compared to the rectus muscles in the control group. MMC simultaneously caused an inflammatory response as well as nanostructural and biomechanical property changes in the collagen fibril network.

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Corresponding author

* Corresponding author. E-mail: sigmoidus@khu.ac.kr

References

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Anduze, A.L. & Burnett, J.M. (1996). Indications for and complications of mitomycin-C in pterygium surgery. Ophthalmic Surg Lasers 27, 667673.
Brooks, S.E., Ribeiro, G.B., Archer, S.M., Elner, V.M. & Del Monte, M.A. (1996). Fat adherence syndrome treated with intraoperative mitomycin-C: A rabbit model. J Pediatr Ophthalmol Strabismus 33, 2127.
Cengiz, Y., Altintas, O., Manav Ay, G. & Cağlar, Y. (2005). Comparison of the effectiveness of mitomycin-C and Viscoat on delayed adjustable strabismus surgery in rabbits. Eur J Ophthalmol 15, 530535.
Chen, P.L., Chen, W.Y. & Lu, D.W. (2005). Evaluation of mitomycin C in reducing postoperative adhesions in strabismus surgery. J Ocul Pharmacol Ther 21, 406410.
Choi, H.Y., Lee, J.H., Lee, J.E. & Jung, J.H. (2010). Effect of bevacizumab on strabismus surgery in rabbits. Invest Ophthalmol Vis Sci 51, 45854588.
Choi, S., Lee, S.J., Shin, J.H., Cheong, Y., Lee, H.J., Paek, J.H., Kim, J.S., Jin, K.H. & Park, H.K. (2011). Ultrastructural investigation of intact orbital implant surfaces using atomic force microscopy. Scanning 33, 211221.
Majmudar, P.A., Forstot, S.L., Dennis, R.F., Nirankari, V.S., Damiano, R.E., Brenart, R. & Epstein, R.J. (2000). Topical mitomycin-C for subepithelial fibrosis after refractive corneal surgery. Ophthalmology 107, 8994.
Mamede, F.V., Laus, J.L., Cabral, V.P., Vicenti, F.A. & Barbieri-Neto, J. (2004). Use of topical mitomycin C in myoplasty of the medial rectus muscle of rabbits. Vet Ophthalmol 7, 335342.
Minguini, N., Monteiro de Carvalho, K.M., Akaishi, P.M. & De Luca, I.M. (2000). Histologic effect of mitomycin C on strabismus surgery in the rabbit. Invest Ophthalmol Vis Sci 41, 33993401.
Wan Norliza, W.M., Raihan, I.S., Azwa, J.A. & Ibrahim, M. (2006). Scleral melting 16 years after pterygium excision with topical mitomycin C adjuvant therapy. Cont Lens Anterior Eye 29, 165167.
Yan, L., Sun, Y., Wang, J., Dai, S., Feng, X., Jiang, B., Wang, Q., Yu, T., Shi, X. & Yang, J. (2010). The effect of mitomycin C in reducing intraarticular adhesion after knee surgery in rabbits. Eur J Pharmacol 643, 15.

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