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Self-Organizing Tissue-Engineered Constructs in Collagen Hydrogels

Published online by Cambridge University Press:  04 January 2012

Robert G. Gourdie ,
Tereance A. Myers ,
Alex McFadden ,
Yin-xiong Li  and
Jay D. Potts
Robert G. Gourdie*
Affiliation:
Department of Regenerative Medicine and Cell Biology, Clemson-MUSC Bioengineering Program, MUSC, Charleston, SC 29425, USA
Tereance A. Myers
Affiliation:
Department of Regenerative Medicine and Cell Biology, Clemson-MUSC Bioengineering Program, MUSC, Charleston, SC 29425, USA
Alex McFadden
Affiliation:
Department of Cell Biology and Anatomy, Program in Bioengineering, University of South Carolina, School of Medicine, Columbia, SC 29209, USA
Yin-xiong Li
Affiliation:
South China Institute of Stem Cell & Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
Jay D. Potts*
Affiliation:
Department of Cell Biology and Anatomy, Program in Bioengineering, University of South Carolina, School of Medicine, Columbia, SC 29209, USA
*
Corresponding author. E-mail: gourdier@musc.edu
Corresponding author. E-mail: jay.potts@uscmed.sc.edu
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Abstract

A novel self-organizing behavior of cellularized gels composed of collagen type 1 that may have utility for tissue engineering is described. Depending on the starting geometry of the tissue culture well, toroidal rings of cells or hollow spheroids were prompted to form autonomously when cells were seeded onto the top of gels and the gels released from attachment to the culture well 12 to 24 h after seeding. Cells within toroids assumed distinct patterns of alignment not seen in control gels in which cells had been mixed in. In control gels, cells formed complex three-dimensional arrangements and assumed relatively higher levels of heterogeneity in expression of the fibronectin splice variant ED-A—a marker of epithelial mesenchymal transformation. The tissue-like constructs resulting from this novel self-organizing behavior may have uses in wound healing and regenerative medicine, as well as building blocks for the iterative assembly of synthetic biological structures.

Keywords

collagentissue engineeringself-organizingepithelial-mesenchymal transformation
Type
Feature Article
Information
Microscopy and Microanalysis , Volume 18 , Issue 1 , February 2012 , pp. 99 - 106
Copyright
Copyright © Microscopy Society of America 2012

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References

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Supplementary material: PDF

Gourdie Supplementary Figure

Supplementary Figure 1. Examples in which LECs were seeded on top collagen gels and formed toroids

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