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Remineralization of Demineralized Enamel by Toothpastes: A Scanning Electron Microscopy, Energy Dispersive X-Ray Analysis, and Three-Dimensional Stereo-Micrographic Study

Published online by Cambridge University Press:  09 May 2013

Elizabeta S. Gjorgievska*
Affiliation:
Faculty of Dental Medicine, University “Sts Cyril and Methodius” Skopje, Republic of Macedonia
John W. Nicholson
Affiliation:
St Mary's University College, Twickenham, London, UK
Ian J. Slipper
Affiliation:
School of Science, University of Greenwich, Chatham, Kent, UK
Marija M. Stevanovic
Affiliation:
Faculty of Dental Medicine, University “Sts Cyril and Methodius” Skopje, Republic of Macedonia
*
*Corresponding author. E-mail: egjorgievska@stomfak.ukim.edu.mk
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Abstract

Remineralization of hard dental tissues is thought to be a tool that could close the gap between prevention and surgical procedures in clinical dentistry. The purpose of this study was to examine the remineralizing potential of different toothpaste formulations: toothpastes containing bioactive glass, hydroxyapatite, or strontium acetate with fluoride, when applied to demineralized enamel. Results obtained by scanning electron microscopy (SEM) and SEM/energy dispersive X-ray analyses proved that the hydroxyapatite and bioactive glass-containing toothpastes were highly efficient in promoting enamel remineralization by formation of deposits and a protective layer on the surface of the demineralized enamel, whereas the toothpaste containing 8% strontium acetate and 1040 ppm fluoride as NaF had little, if any, remineralization potential. In conclusion, the treatment of demineralized teeth with toothpastes containing hydroxyapatite or bioactive glass resulted in repair of the damaged tissue.

Type
Biological Applications
Copyright
Copyright © Microscopy Society of America 2013 

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References

Arends, J., Ruben, J. & Dijkman, A.G. (1990). The effect of fluoride release from a fluoride containing composite resin on secondary caries: An in vitro study. Quintessence Int 21, 671674.Google ScholarPubMed
Earl, J.S., Ward, M.B. & Langford, R.M. (2010). Investigation of dentinal tubule occlusion using FIB-SEM milling and EDX. J Clin Dent 21, 3741.Google ScholarPubMed
Elliott, J.C. (1997). Structure, crystal chemistry and density of enamel apatites. Ciba Found Symp 205, 5467.Google ScholarPubMed
Gjorgievska, E.S. & Nicholson, J.W. (2010). A preliminary study of enamel remineralization by dentifrices based on Recaldent (CPP-ACP) and Novamin (calcium-sodium-phosphosilicate). Acta Odontol Latinoam 23, 234239.Google ScholarPubMed
Gjorgievska, E. & Nicholson, J.W. (2011). Prevention of enamel demineralization after tooth bleaching by bioactive glass incorporated into toothpaste. Aust Dent J 56, 193200.CrossRefGoogle ScholarPubMed
Gjorgievska, E.S., Nicholson, J.W., Iljovska, S. & Slipper, I. (2009). The potential of fluoride-releasing restoratives to inhibit enamel demineralization: An SEM study. Contributions, Sec Biol Med Sci MASA 30, 191203.Google ScholarPubMed
Hench, L.L. & Andersson, O.H. (1993). Bioactive glasses. In Introduction to Bioceramics, Wilson, J. (Ed.), pp. 4162. Singapore: World Science Publishing Company.CrossRefGoogle Scholar
Hench, L.L., Clark, A.E., Schaake, J.R. & Schaake, H.F. (1972). Effects of microstructure on the radiation stability of amorphous semiconductors. J Non-Cryst Solids 810, 837843.CrossRefGoogle Scholar
Jeong, S.H., Jang, S.O., Kim, K.N., Kwon, H.K., Park, Y.D. & Kim, B.I. (2006). Remineralization potential of new toothpaste containing nano-hydroxyapatite. Key Eng Mater 309311, 537540.CrossRefGoogle Scholar
Lovelace, T.B., Mellonig, J.T., Meffert, R.M., Jones, A.A., Nummikoski, P.V. & Cochran, D.L. (1998). Clinical evaluation of bioactive glass in the treatment of periodontal osseous defects in humans. J Periodontol 69, 10271035.CrossRefGoogle ScholarPubMed
Markowitz, K. (2009). The original desensitizers: Strontium and potassium salts. J Clin Dent 20, 145151.Google ScholarPubMed
Nishimura, K., Yamaguchi, Y. & Yoshitake, K. (1999). Demineralized enamel surface microstructure after brushing using toothpaste containing medical hydroxyapatite under FE-SEM observation. J Japan Stomatol Soc 48, 199210.Google Scholar
Niwa, M., Sato, T., Li, W., Aoki, H., Aoki, H. & Daisaku, T. (2001). Polishing and whitening properties of toothpaste containing hydroxyapatite. J Mater Sci Mater Med 12, 277281.CrossRefGoogle ScholarPubMed
Paes Leme, A.F., Dalcico, R., Tabchoury, C.P., Del Bel Cury, A.A., Rosalen, P.L. & Cury, J.A. (2004). In situ effect of frequent sucrose exposure on enamel demineralization and on plaque composition after APF application and F dentifrice use. J Dent Res 83, 7175.CrossRefGoogle ScholarPubMed
Park, J.J., Park, J.B., Kwon, Y.H., Herr, Y. & Chung, J.H. (2005). The effects of microcrystalline hydroxyapatite containing toothpaste in the control of tooth hypersensitivity. J Korean Acad Periodontol 35, 577590.CrossRefGoogle Scholar
Peters, M.C. (2010). Strategies for noninvasive demineralized tissue repair. Dent Clin N Am 54, 507525.CrossRefGoogle ScholarPubMed
Pradeep, K. & Prasanna, K.R. (2011). Remineralizing agents in the non-invasive treatment of early carious lesions. Int J Dent Case Reports 1, 7384.Google Scholar
Reynolds, E.C. (2008). Calcium phosphate-based remineralization systems: Scientific evidence? Aust Dent J 53, 268273.CrossRefGoogle ScholarPubMed
Salonen, J.I., Arjasmaa, M., Tuominen, U., Behbehani, M.J. & Zaatar, E.I. (2009). Bioactive glass in dentistry. J Minim Interv Dent 2, 208218.Google Scholar
Sheiham, A. (2006). Dental caries affects body weight, growth and quality of life in preschool children. Br Dent J 25, 625626.CrossRefGoogle Scholar
Shellis, R.P., Wahab, F.K. & Heywood, B.R. (1993). The hydroxyapatite ion activity product in acid solutions equilibrated with human enamel at 37 degrees. Caries Res 27, 365372.CrossRefGoogle ScholarPubMed
Shellis, R.P. & Wilson, R.M. (2004). Apparent solubility distributions of hydroxyapatite and enamel apatite. J Colloid Interf Sci 278, 325332.CrossRefGoogle ScholarPubMed
ten Cate, J.M. (1999). Current concepts on the theories of the mechanism of action of fluoride. Acta Odontol Scand 57, 325329.CrossRefGoogle ScholarPubMed
ten Cate, J.M. & Featherstone, J.D. (1991). Mechanistic aspects of the interactions between fluoride and dental enamel. CRC Crit Rev Oral Biol Med 2, 283296.CrossRefGoogle ScholarPubMed
Tschoppe, P., Zandim, D.L., Martus, P. & Kielbassa, A.M. (2011). Enamel and dentine remineralization by nano-hydroxyapatite toothpastes. J Dent 39, 430437.CrossRefGoogle ScholarPubMed
Vollenweider, M., Brunner, T.J., Knecht, S., Grass, R.N., Zehnder, M., Imfeld, T. & Stark, W.J. (2007). Remineralization of human dentin using ultrafine bioactive glass particles. Acta Biomater 3, 936943.CrossRefGoogle ScholarPubMed
Walsh, L.J. (2009). Contemporary technologies for remineralization therapies: A review. Int Dent SA 6, 616.Google Scholar
Wang, Z., Jiang, T., Sauro, S., Pashley, D.H., Toledano, M., Osorio, R., Liang, S., Xing, W., Sa, Y. & Wang, Y. (2011). The dentine remineralization activity of a desensitizing bioactive glass-containing toothpaste: An in vitro study. Aust Dent J 56, 372381.CrossRefGoogle ScholarPubMed
Wilson, J., Clark, A.E., Dou, E., Crier, J., Smith, W.K. & Summit, J.S. (1994). Clinical applications of bioglass implants. In Bioceramics, Andersson, O.H. & Happonen, R.P. (Eds.), vol. 7, pp. 415422. Cambridge: Butterworth-Heinemann.CrossRefGoogle Scholar
Xue, J., Li, W. & Swain, M.V. (2009). In vitro demineralization of human enamel natural and abraded surfaces: A micromechanical and SEM investigation. J Dent 37, 264272.CrossRefGoogle ScholarPubMed
Zero, D.T. (2006). Dentifrices, mouthwashes, and remineralization/caries arrestment strategies. BMC Oral Health 6, S9S22.CrossRefGoogle ScholarPubMed