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Recent Development of Cone-Beam X-Ray Microtomography at Sunyab

Published online by Cambridge University Press:  02 July 2020

S.J. Pan ,
A. Shih ,
W.S. Liou ,
M.S. Park ,
G. Wang ,
B.A. Bohne ,
S.P. Newberry ,
H. Kim  and
P.C. Cheng
S.J. Pan
Affiliation:
AMIL, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY14260USA
A. Shih
Affiliation:
AMIL, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY14260USA
W.S. Liou
Affiliation:
AMIL, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY14260USA
M.S. Park
Affiliation:
AMIL, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY14260USA
G. Wang
Affiliation:
Dept. of Radiology, Univ. of Iowa, Iowa City, IA52242, USA
B.A. Bohne
Affiliation:
Dept. of Otolaryngology, Washington Univ. Medical School, St. Louis, MO63110, USA
S.P. Newberry
Affiliation:
CBI Labs, Box 11, S. Wescott Rd, Schenectady, NY12306, USA
H. Kim
Affiliation:
Dept. of Material Sciences and Eng., Kwangju Inst, of Science and Technology, Korea
P.C. Cheng
Affiliation:
AMIL, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY14260USA
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An experimental X-ray cone-beam microtomographic imaging system utilizing a generalized Feldkamp reconstruction algorithm has been developed in our laboratory. This microtomographic imaging system consists of a conventional dental X-ray source (Aztech 65, Boulder, CO), a sample position and rotation stage, an X-ray scintillation phosphor screen, and a high resolution slow scan cooled CCD camera (Kodak KAF 1400). A generalized Feldkamp cone-beam algorithm was used to perform tomographic reconstruction from cone-beam projection data. This algorithm was developed for various hardware configuration to perform reconstruction of spherical, rod-shaped and plate-like specimen.

A test sample consists of 8 glass beads (approx. 800μm in diameter) dispersed in an epoxy-filled #0 gelatin capsule. One hundred X-ray projection images were captured equal angularly (at 3.6 degree spacing) by the cooled CCD camera at a of 1317×967 (17×17mm2) pixels with 12-bit dynamic range. Figure 1 shows a 3D isosurface rendering of the test sample. The eight glass beads and trapped air bubbles (arrows) in the epoxy resin (e) are clearly visible.

Type
Computational Advances and Enabling Technologies for 3D Microscopies in Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 1125 - 1126
Copyright
Copyright © Microscopy Society of America 1997

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References

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