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Quantitative Single-Cell Gene Expression Measurements in Bacteria Using Time-Lapse Microscopy

Published online by Cambridge University Press:  27 August 2014

Mary J. Dunlop*
Affiliation:
School of Engineering, University of Vermont, Burlington, VT, USA

Abstract

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Type
Abstract
Copyright
Copyright © Microscopy Society of America 2014 

References

1. Choi, PJ, Cai, L, Frieda, K, Xie, S (2008) A stochastic single-molecule event triggers phenotype switching of a bacterial cell. Science (New York, NY) 322: 442-446.Google Scholar
2. Locke, JCW, Young, JW, Fontes, M, Hernández Jiménez, MJ, Elowitz, MB (2011) Stochastic pulse regulation in bacterial stress response. Science (New York, NY) 334: 366-369.Google Scholar
3. Cai, L, Dalal, CK, Elowitz, MB (2008) Frequency-modulated nuclear localization bursts coordinate gene regulation. Nature 455: 485-U416.Google Scholar
4. Hansen, AS O'Shea EK (2013) Promoter decoding of transcription factor dynamics involves a trade-off between noise and control of gene expression. Molecular Systems Biology 9: 704.Google Scholar
5. Hao, N, Budnik, BA, Gunawardena, J, O’Shea, EK (2013) Tunable signal processing through modular control of transcription factor translocation. Science (New York, NY) 339: 460-464.Google Scholar
6. Locke, JCW, Elowitz, MB (2009) Using movies to analyse gene circuit dynamics in single cells. Nature Reviews Microbiology 383-392.Google Scholar
7. Young, JW, Locke, JCW, Altinok, A, Rosenfeld, N, Bacarian, T, et al.. (2012) Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy. Nature Protocols 7: 80-88.Google Scholar