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Protocol Development for 3D Reconstructions: Combining In Vivo Imaging, APEX2 and En Bloc Staining of Mouse Visual Cortex

Published online by Cambridge University Press:  23 September 2015

JoAnn Buchanan ,
Marc Takeno ,
Tanya Daigle ,
Adam Bleckert ,
Daniel Bumbarger ,
Agnes Bodor ,
Derrick Brittain ,
Ali Cetin  and
Nuno Macarico da Costa
JoAnn Buchanan
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Marc Takeno
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Tanya Daigle
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Adam Bleckert
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Daniel Bumbarger
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Agnes Bodor
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Derrick Brittain
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Ali Cetin
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Nuno Macarico da Costa
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 21 , Supplement S3: Proceedings of Microscopy & Microanalysis 2015 , August 2015 , pp. 1275 - 1276
Copyright
Copyright © Microscopy Society of America 2015 

References

[1] Lam, S., et al., Nature Methods 12 (2015). p 51.Google Scholar
[2] Martell, J., et al., Nature Biotechnology 30 (2012). p 1243.Google Scholar
[3] Mikula, S., Binding, J. & Denk, W., Nature Methods 9 (2012). p 1198.Google Scholar
[4] The authors are grateful to the Allen Institute founders, P.G. Allen and J. Allen, for their vision, encouragement, and support..Google Scholar