Skip to main content Accessibility help
×
Home

Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles

  • Sėan K. Mulligan (a1), Jeffrey A. Speir (a1), Ivan Razinkov (a1) (a2), Anchi Cheng (a1) (a2), John Crum (a1), Tilak Jain (a1), Erika Duggan (a3), Er Liu (a4), John P. Nolan (a3), Bridget Carragher (a1) (a2) and Clinton S. Potter (a1) (a2)...

Abstract

We describe a system for rapidly screening hundreds of nanoparticle samples using transmission electron microscopy (TEM). The system uses a liquid handling robot to place up to 96 individual samples onto a single standard TEM grid at separate locations. The grid is then transferred into the TEM and automated software is used to acquire multiscale images of each sample. The images are then analyzed to extract metrics on the size, shape, and morphology of the nanoparticles. The system has been used to characterize plasmonically active nanomaterials.

Copyright

Corresponding author

* Corresponding author. cpotter@nysbc.org

Footnotes

Hide All

These authors contributed equally to this work.

Footnotes

References

Hide All
Ali, M.R.K., Snyder, B. & El-Sayed, M.A (2012). Synthesis and optical properties of small Au nanorods using a seedless growth technique. Langmuir 28(25), 98079815.
Alkilany, A.M., Lohse, S.E. & Murphy, C.J. (2013). The gold standard: Gold nanoparticle libraries to understand the nanobio interface. Acc Chem Res 46(3), 650661.
Castro-Hartmann, P., Heck, G., Eltit, J.M., Fawcett, P. & Samso, M. (2013). The ArrayGrid: A methodology for applying multiple samples to a single TEM specimen grid. Ultramicroscopy 135(December), 105112.
Cheng, A., Leung, A., Fellmann, D., Quispe, J., Suloway, C., Pulokas, J., Abeyrathne, P.D., Lam, J.S., Carragher, B. & Potter, C.S. (2007). Towards automated screening of two-dimensional crystals. J Struct Biol 160(3), 324331.
Coudray, N., Hermann, G., Caujolle-Bert, D., Karathanou, A., Erne-Brand, F., Buessler, J.-L., Daum, P., Plitzko, J.M., Chami, M., Mueller, U., Kihl, H., Urban, J.-P., Engel, A. & Rmigy, H.-W. (2011). Automated screening of 2D crystallization trials using transmission electron microscopy: A high-throughput tool-chain for sample preparation and microscopic analysis. J Struct Biol 173(2), 365374.
Deegan, R.D., Bakajin, O., Dupont, T.F., Huber, G., Nagel, S.R. & Witten, T.A. (1997). Capillary flow as the cause of ring stains from dried liquid drops. Nature 389(October), 827829.
Hu, M., Vink, M., Kim, C., Derr, K.D., Koss, J., D’Amico, K., Cheng, A., Pulokas, J., Ubarretxena-Belandia, I. & Stokes, D. (2010). Automated electron microscopy for evaluating two-dimensional crystallization of membrane proteins. J Struct Biol 171(1), 102110.
Jain, T., Sheehan, P., Crum, J., Carragher, B. & Potter, C.S. (2012). Spotiton: A prototype for an integrated inkjet dispense and vitrification system for cryo-TEM. J Struct Biol 179(1), 6875.
Kamentsky, L., Jones, T.R., Fraser, A., Bray, M.-A., Logan, D.J., Madden, K.L., Ljosa, V., Rueden, C., Eliceiri, K.W. & Carpenter, A.E. (2011). Improved structure, function, and compatibility for CellProfiler: Modular high-throughput image analysis software. Bioinformatics 27(8), 11791180.
Krafft, C. & Popp, J. (2015). The many facets of Raman spectroscopy for biomedical analysis. Anal Bioanal Chem 407(3), 699717.
Lohse, S.E. & Murphy, C.J. (2013). The quest for shape control: A history of gold nanorod synthesis. Chem Mater 25(8), 12501261.
Moon, H.R., Lim, D.-W. & Suh, M.P. (2013). Fabrication of metal nanoparticles in metalorganic frameworks. Chem Soc Rev 42(4), 18071824.
Nolan, J.P., Duggan, E. & Condello, D. (2014). Optimization of SERS tag intensity, binding footprint, and emittance. Bioconjug Chem 25(7), 12331242.
Nolan, J.P., Duggan, E., Liu, E., Condello, D., Dave, Isha & Stoner, S.A (2012). Single cell analysis using surface enhanced Raman scattering (SERS) tags. Methods 57(3), 272279.
Orendorff, C.J., Gearheart, L., Jana, N.R. & Murphy, C.J. (2006). Aspect ratio dependence on surface enhanced Raman scattering using silver and gold nanorod substrates. Phys Chem Chem Phys 8(1), 165170.
Potter, C.S., Chu, H., Frey, B., Green, C., Kisseberth, N., Madden, T.J., Miller, K.L., Nahrstedt, K., Pulokas, J., Reilein, A., Tcheng, D., Weber, D. & Carragher, B. (1999). Leginon: A system for fully automated acquisition of 1000 electron micrographs a day. Ultramicroscopy 77(3–4), 153161.
Potter, C.S., Pulokas, J., Smith, P., Suloway, C. & Carragher, B. (2004). Robotic grid loading system for a transmission electron microscope. J Struct Biol 146(3), 431440.
Rao, C.N., Ramakrishna Matte, H.S., Voggu, R. & Govindaraj, A. (2012). Recent progress in the synthesis of inorganic nanoparticles. Dalton Trans 41(17), 50895120.
Rodriguez-Lorenzo, L., Fabris, L. & Alvarez-Puebla, R.A. (2012). Multiplex optical sensing with surface-enhanced Raman scattering: A critical review. Anal Chim Acta 745, 1023.
Smith, D.K., Miller, N.R., Korgel, B.A. 2009). Iodide in CTAB prevents gold nanorod formation. Langmuir 25(16), 95189524.
Striemer, C.C., Gaborski, T.R., McGrath, J.L. & Fauchet, P.M. (2007). Charge- and size-based separation of macromolecules using ultrathin silicon membranes. Nature 445(7129), 749753.
Suloway, C., Pulokas, J., Fellmann, D., Cheng, A., Guerra, F., Quispe, J., Stagg, S., Potter, C.S., Carragher, B. (2005). Automated molecular microscopy: The new Leginon system. J Struct Biol 151(1), 4160.
Vo-Dinh, T., Fales, A.M., Griffin, G.D., Khoury, C.G., Liu, Y., Ngo, H., Norton, S.J., Register, J.K., Wang, H.-N. & Yuan, H. (2013). Plasmonic nanoprobes: From chemical sensing to medical diagnostics and therapy. Nanoscale 5(21), 1012710140.
Yunker, P.J., Still, T., Lohr, M.A. & Yodh, A.G. (2011). Suppression of the coffee-ring effect by shape-dependent capillary interactions. Nature 476(7360), 308311.

Keywords

Type Description Title
VIDEO
Supplementary materials

Mulligan Supplementary Material
Supplementary Movie

 Video (1.6 MB)
1.6 MB

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed