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Multi-modal / Multi-resolution Neural Circuit Reconstruction with Automated Tape Ultramicrotomy (ATUM) and Serial Section Scanning Electron Microscopy

Published online by Cambridge University Press:  30 July 2020

Karl Friedrichsen ,
Pratyush Ramakrishna ,
Jen Chun Hsiang ,
Daniel Kerschensteiner  and
Josh Morgan
Karl Friedrichsen
Affiliation:
Washington University in St. Louis, St. Louis, Missouri, United States
Pratyush Ramakrishna
Affiliation:
Washington University in St. Louis, St. Louis, Missouri, United States
Jen Chun Hsiang
Affiliation:
Washington University in St. Louis, St. Louis, Missouri, United States
Daniel Kerschensteiner
Affiliation:
Washington University in St. Louis, St. Louis, Missouri, United States
Josh Morgan
Affiliation:
Washington University in St. Louis, St. Louis, Missouri, United States

Abstract

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Type
3D Scanning Electron Microscopy Imaging of Biological Samples
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 592 - 593
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Copyright
Copyright © Microscopy Society of America 2020

References

Hayworth, K.J., Morgan, J.L., Schalek, R., Berger, D.R., Hildebrand, D.G.C., and Lichtman, J.W. (2014). Imaging ATUM ultrathin section libraries with WaferMapper: A multi-scale approach to EM reconstruction of neural circuits. Front. Neural Circuits 8.10.3389/fncir.2014.00068CrossRefGoogle Scholar
Schalek, R., Hayworth, K., Kasthuri, N., Morgan, J.L., Berger, D., Wilson, A.M., Anger, P., Aderhold, D., Seung, H.S., and Lichtman, J.W. (2012). ATUM-based SEM for High-Speed Large-Volume Biological Reconstructions. Microsc. Microanal. 18.Google Scholar