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Mice Spermatogonial Stem Cells Transplantation Induces Macrophage Migration into the Seminiferous Epithelium and Lipid Body Formation: High-Resolution Light Microscopy and Ultrastructural Studies

Published online by Cambridge University Press:  03 November 2011

Felipe F. Dias ,
Hélio Chiarini-Garcia ,
Gleydes G. Parreira  and
Rossana C.N. Melo
Felipe F. Dias
Affiliation:
Laboratory of Cellular Biology, Department of Biology, Federal University of Juiz de Fora, UFJF, Juiz de Fora, MG, Brazil Laboratory of Structural Biology and Reproduction, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
Hélio Chiarini-Garcia
Affiliation:
Laboratory of Structural Biology and Reproduction, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
Gleydes G. Parreira
Affiliation:
Laboratory of Structural Biology and Reproduction, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
Rossana C.N. Melo*
Affiliation:
Laboratory of Cellular Biology, Department of Biology, Federal University of Juiz de Fora, UFJF, Juiz de Fora, MG, Brazil
*
Corresponding author. E-mail: rossana.melo@ufjf.edu.br
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Abstract

Transplantation of spermatogonial stem cells (SSCs), the male germline stem cells, in experimental animal models has been successfully used to study mechanisms involved in SSC self-renewal and to restore fertility. However, there are still many challenges associated with understanding the recipient immune response for SSCs use in clinical therapies. Here, we have undertaken a detailed structural study of macrophages elicited by SSCs transplantation in mice using both high-resolution light microscopy (HRLM) and transmission electron microscopy (TEM). We demonstrate that SSCs transplantation elicits a rapid and potent recruitment of macrophages into the seminiferous epithelium (SE). Infiltrating macrophages were derived from differentiation of peritubular monocyte-like cells into typical activated macrophages, which actively migrate through the SE, accumulate in the tubule lumen, and direct phagocytosis of differentiating germ cells and spermatozoa. Quantitative TEM analyses revealed increased formation of lipid bodies (LBs), organelles recognized as intracellular platforms for synthesis of inflammatory mediators and key markers of macrophage activation, within both infiltrating macrophages and Sertoli cells. LBs significantly increased in number and size in parallel to the augmented macrophage migration during different times post-transplantation. Our findings suggest that LBs may be involved with immunomodulatory mechanisms regulating the seminiferous tubule niche after SSC transplantation.

Keywords

spermatogonial stem cellsgerm cell transplantationmacrophage migrationlipid bodieslipid dropletsinflammationelectron microscopyhigh-resolution light microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 6 , December 2011 , pp. 1002 - 1014
Copyright
Copyright © Microscopy Society of America 2011

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Supplementary material: PDF

Dias Supplementary Figure 1

Supplementary Figure 1. A representative male germ cell undergoing apoptosis. A degenerating germ cell with morphological features of apoptosis such as condensation of the nucleus, cell retraction, and increased electron density of the cytoplasmic matrix is seen at the tubular lumen in a spermatogonial stem cell-transplanted Wv/Wv mouse. L, lumen; SE, seminiferous epithelium. Scale bar, 5 mm.

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