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Localization of Nitric Oxide Synthase in the Salivary Gland Ducts of Feeding Female Dermacentor Variabilis (SAY) and the Effect of Endogenous Nitric Oxide on Isolated Salivary Gland Ducts

Published online by Cambridge University Press:  02 July 2020

William J. Lamoreaux ,
Malinda E. C. Fitzgerald ,
Glen R. Needham ,
Sharon Frase ,
Nabil Bayakly  and
Lewis B. Coons
William J. Lamoreaux
Affiliation:
Department of Anatomy and Neurobiology, The University of Tennessee, Memphis, 855 Monroe, Memphis, TN38163
Malinda E. C. Fitzgerald
Affiliation:
Department of Anatomy and Neurobiology, The University of Tennessee, Memphis, 855 Monroe, Memphis, TN38163
Glen R. Needham
Affiliation:
The Acarology Laboratory, Department of Entomology, The Ohio State University, 211 Biological Sciences Building 454 West 12th Street, ColumbusOH43210
Sharon Frase
Affiliation:
Integrated Microscopy Center, Life Sciences 101, Campus Box 526040, The University of Memphis, MemphisTN38152-6040
Nabil Bayakly
Affiliation:
Integrated Microscopy Center, Life Sciences 101, Campus Box 526040, The University of Memphis, MemphisTN38152-6040
Lewis B. Coons
Affiliation:
Integrated Microscopy Center, Life Sciences 101, Campus Box 526040, The University of Memphis, MemphisTN38152-6040
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Extract

The tick Dermacentor variabilis returns much of the water and ions from the bloodmeal back into the host as saliva produced in paired salivary glands. These glands are made up of acini and a system of ducts (Fig. 1). Bloodsucking alternates with salivation, but saliva is constantly produced by the acini and expelled into the ducts where it accumulates during bloodsucking. Ducts consist of a layer of epithelial cells, a cuticular coil, and an epicuticular lining of the lumen (Figs. 2, 3). Actin-like filaments run from the coil into the epithelial cells as shown by TEM and CLSM (Figs. 3, 4). These filaments are absent in cytochalasin D treated ducts.

Nitric oxide synthase (=NADPH diaphorase) is present in duct epithelial cells as shown by histochemistry (Fig. 5). We measured nitric oxide (NO) indirectly by the conversion of L-arginine to L-citrulline. NO production and production of saliva is dopamine dependent. NO activates guanylyl cyclase as measured by an RIA for cGMP. The lumen width in dopamine stimulated isolated ducts increased by 19.7% as measured optically. Ducts pre-treated with 10 μM L-NAME, an NOS specific inhibitor, showed no significant increase in width. The addition of 10 μM L-arginine to these ducts partially restored the increase in lumen diameter.

Type
Non-Vertebrate Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 115 - 116
Copyright
Copyright © Microscopy Society of America 1997

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References

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5. This work was supported by NIH Grant AI 33665.Google Scholar