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Iridovirus-Like Viruses in Erythrocytes of Lacertids from Portugal

  • António P. Alves de Matos (a1) (a2), M. Filomena Caeiro (a1), Filipa F. Vale (a1) (a3), Eduardo Crespo (a4) and Ilan Paperna (a5)...


Icosahedral nucleo-cytoplasmic large DNA viruses (NCLDV)-like viruses, which form inclusions in the erythrocyte cytoplasm of reptiles, were previously presented as candidates for a new genus of the Iridoviridae family. The present work describes the distribution of infected lizard hosts and ultrastructural characteristics of the viral inclusions of NCLDV-like viruses from Portugal and adjacent locations in Spain. Giemsa-stained blood smears of 235 Lacerta schreiberi from Portugal and Spain, 571 Lacerta monticola from the mountain Serra da Estrela (Portugal), 794 Podarcis hispanica from several localities in Portugal and Spain, and 25 Lacerta dugesii from Madeira Island, were studied. Infection in L. schreiberi was only found in mountain populations, up to 30% in Serra da Estrela and 9–11% elsewhere. It was absent in lizards from lowlands. Prevalence of infection among L. monticola in Serra da Estrela was 10%; infected lizards were found during March to July and October but not in August and September. Infection in P. hispanica was below 3.3%. Only one infected specimen of L. dugesii was identified by light microscopy. Ultrastructural examination of infected samples revealed that the inclusions are virus assembly sites of icosahedral cytoplasmic iridovirus-like virions. Virions from different host species have different ultrastructural features and probably represent different related viruses.


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Alves de Matos, A.P., Caeiro, M.F., Papp, T., Matos, B.A., Correia, A.C. & Marshang, R.E. (2011). New viruses from Lacerta monticola (Serra da Estrela, Portugal): Further evidence for a new group of nucleo-cytoplasmic large deoxyriboviruses (NCLDVs). Microsc Microanal 17, 101108.
Alves de Matos, A.P. & Paperna, I. (1993a). Ultrastructural study of Pirhemocyton virus in lizard erythrocytes. Ann Parasitol Hum Comp 68, 2433.
Alves de Matos, A.P. & Paperna, I. (1993b). Ultrastructure of erythrocytic virus of the South African anuran Ptychadena anchietae . J Aquatic Dis 16, 105109.
Alves de Matos, A.P., Paperna, I. & Crespo, E. (2002). Experimental infection of lacertids with lizard erythrocytic viruses. Intervirology 45, 150159.
Arcay de Peraza, L., Nazir, P. & Diaz, M.T. (1971). The “paranuclear corpuscules” in poikilothermal vertebrates. I. Description of a new species of Toddia from Iguana iguana in Venezuela. Acta Biol Ven 7, 191199.
Blanc, G. & Ascione, L. (1958). Sur un parasite endoglobulaire du lézard Eremias guttulatus olivieri Audoin de la région de Marrakech (Maroc). Bull Soc Path Exot 51, 508510.
Brumpt, E. & Lavier, G. (1935). Sur un hematozoaire nouveau du lézard vert, Pirhemocyton lacertae n. sp. Ann Parasitol 13, 537543.
Brygoo, E.R. (1963). Remarques sur les Protozoaires du sang de Caméléons malgaches. Ann Parasitol 38, 636637.
Chatton, E. & Blanc, G. (1914). Sur un hematozoaire noveau, Pirhemocyton tarentolae du Gecko, Tarentola mauritanica, et sur les alterations globulaires qu'il determine. C R Soc Biol 77, 496498.
Chinchar, V.G., Hyatt, A., Miyazaki, T. & Williams, T. (2009). Family Iridoviridae: Poor viral relations no longer. Curr Top Microbial Immunol 328, 123170.
Dales, S. & Mosbach, E.H. (1968). Vaccinia as a model for membrane biogenesis. Virology 35, 564583.
Devauchelle, G., Stoltz, D.B. & Darcy-Tripier, F. (1985). Comparative ultrastructure of Iridoviridae. In Iridoviridae, Willis, D.B. (Ed.), pp. 12. Berlin, Heidelberg, New York: Springer-Verlag.
Glenn, J.A. & Emmenegger, E.J. (2012). Kinetics of viral load and erythrocytic inclusion body formation in pacific herring artificially infected with erythrocytic necrosis virus. J Aquat Anim Health 24, 195200.
Gruya-Gray, J., Petric, M. & Desser, S.S. (1989). Ultrastructural, biochemical, and biological properties of an erythrocytic virus of frogs from Ontario, Canada. In Viruses of Lower Vertebrates, Ahne, W. & Kurstak, E. (Eds.), pp. 6978. Berlin, Heidelberg, New York: Springer-Verlag.
Hanson, L.A., Rudis, M.R., Vasquez-Lee, M. & Montgomery, R.D. (2006). A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene. Virol J 3, 28.
Hollinshead, M., Vanderplasschen, A., Smith, G.L., David, J. & Vaux, A.D.J. (1999). Vaccinia virus intracellular mature virions contain only one lipid membrane. J Virol 73, 15031517.
Iyer, L.M., Aravind, L. & Koonin, E.V. (2001). Common origin of four diverse families of large eukaryotic DNA viruses. J Virol 75, 1172011734.
Johnsrude, J.D., Raskin, R.E., Hoge, Y.A. & Erdos, G.W. (1997). Intraerythrocytic inclusions associated with iridoviral infection in a fer de lance (Bothrops moojeni) snake. Vet Pathol 34, 235238.
Johnston, M.R.L. (1975). Distribution of Pirhemocyton Chatton and Blanc and other, possibly related, infections of poikilotherms. J Protozool 22, 529535.
Just, F.T., Essbauer, S.S., Ahne, W. & Blahak, S. (2001). Occurrence of an invertebrate iridescent-like virus (Iridoviridae) in reptiles. J Vet Med B 48, 685694.
Laliberte, J.P. & Moss, B. (2010). Lipid membranes in poxvirus replication. Viruses 2, 972986.
Marco, A. (1997). Lacerta schreiberi Bedriaga, 1878. In Distribución y biogeografía de los anfibios y reptiles en España y Portugal, Monografías de Herpetología, vol. 3, Pleguezuelos, J.M. (Ed.), pp. 228230. Granada: Editorial Universidad de Granada.
Meyers, T.R., Hauck, A.K., Blankenbeckler, W.D. & Minicucci, T. (1986). First report of viral erythrocytic necrosis in Alaska, USA, associated with epizootic mortality in Pacific herring, Clupea harengus pallasi (Valenciennes). J Fish Dis 9, 479491.
Paperna, I. & Alves de Matos, A.P. (1993). Erythrocytic viral infections of lizards and frogs: New hosts, geographical locations and description of the infection process. Ann Parasitol Hum Comp 68, 1123.
Reno, P.W. & Nicholson, B.L. (1981). Ultrastructure and prevalence of viral erythrocytic necrosis (VEN) virus in the Atlantic cod, Gadus morhua L., from the northern Atlantic Ocean. J Fish Dis 4, 361370.
Rodrigues, F.C., Nunes, J.F., Chagas, J.P. & Soares, J.O. (1981). Photographic defocusing: A means to render recognizable line traced models of negatively stained biological specimens. J Microsc 122, 295300.
Salas, M.L. & Andrés, G. (2012). African swine fever virus morphogenesis. Virus Res 173, 2941.
Sousa, M.A. & Weigl, D.R. (1976). The viral nature of Toddia França, 1912. Mem Inst Oswaldo Cruz 74, 213230.
Stehbens, W.E. & Johnston, M.R.L. (1966). The viral nature of Pirhemocyton tarentolae . J Ultrastr Res 15, 543554.
Stoltz, D.B. (1973). The structure of icosahedral cytoplasmic deoxyriboviruses. II. An alternative model. J Ultrastr Res 43, 5874.
Telford, S.R. & Jacobson, E.R. (1993). Lizard erythrocytic virus in East African chameleons. J Wildl Dis 29, 5763.
Walker, R. & Sherburne, S.W. (1977). Piscine erythrocytic necrosis virus in Atlantic cod, Gadus morhua, and other fish: Ultrastructure and distribution. J Fish Res Board Can 34, 11881195.
Wellehan, J.F. Jr., Strik, N.I., Stacy, B.A., Childress, A.L., Jacobson, E.R. & Telford, S.R. Jr. (2008). Characterization of an erythrocytic virus in the family Iridoviridae from a peninsula ribbon snake (Thamnophis sauritus sackenii). Vet Microbiol 131, 115122.
Williams, T., Barbosa-Solomieu, V. & Chinchar, V.G. (2005). A decade of advances in iridovirus research. Adv Virus Res 65, 173248.
Willis, D.B. (1990). Taxonomy of iridoviruses. In Molecular Biology of Iridoviruses, Darai, G. (Ed.), pp. 112. Boston, Dordrecht, London: Kluwer Academic Publishers.



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