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Giant Human T-Lymphocytes (Jurkat Cells) Expressing HIV-1 Tat-72: LM and TEM.

Published online by Cambridge University Press:  02 July 2020

J. Gilloteaux ,
J. Koch ,
J. M. Jamison ,
M. Authelet ,
C. Sowick  and
J.L. Summers
J. Gilloteaux
Affiliation:
Department of Anatomy, Lake Erie College of Ostéopathie Medicine, Erie, PA16509
J. Koch
Affiliation:
Department of Urology, Northeastern Ohio Universities College of Medicine, Rootstown, OH, 44272
J. M. Jamison
Affiliation:
Department of Urology, Northeastern Ohio Universities College of Medicine, Rootstown, OH, 44272
M. Authelet
Affiliation:
Brain Research Unit and Laboratory of Electron Microscopy, Université Libre de Bruxelles, Campus-Anderlecht, B- 1170, Brussels, Belgium
C. Sowick
Affiliation:
Department of Urology, Northeastern Ohio Universities College of Medicine, Rootstown, OH, 44272
J.L. Summers
Affiliation:
Department of Urology, Northeastern Ohio Universities College of Medicine, Rootstown, OH, 44272
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Extract

The human immunodeficiency virus (HIV-1) is dependent upon the synthesis of TAT for efficient transcriptional activation and replication. Following translocation to the nucleus TAT interacts with a cis-acting TAT-responsive element located between nucleotides +1 and +42 within the HIV-1 long terminal repeat. This region contains an RNA stem loop structure TAR which interacts with TAT and cellular proteins to increase transcription 1000 fold We have transfected human T-lymphocytes with an expression vector containing the HIV-1 LTR driving the synthesis of TAT-72. A stable cell line was generated by selection with G418. Cells were grown on round glass coverslips coated with a solution of fibronectin overnight (at 37 °C). In addition to light microscopy (LM) observations, cells on coverslips were fixed with a 3.5% glutaraldehyde buffered solution (0.1 M Na cacodylate) for 5 min at room temperature and gently centrifliged in a microfuge (400 rpm, 3min) to obtain a cellular pellet.

Type
Neoplasia: Abnormal Cell Growth Or Death/Apoptosis? Insights From Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 15 - 16
Copyright
Copyright © Microscopy Society of America 1997

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References

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