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The Effect of Cadmium Under Different Salinity Conditions on the Cellular Architecture and Metabolism in the Red Alga Pterocladiella capillacea (Rhodophyta, Gelidiales)

Published online by Cambridge University Press:  01 July 2014

Marthiellen R. de L. Felix ,
Luz K.P. Osorio ,
Luciane C. Ouriques ,
Francine L. Farias-Soares ,
Neusa Steiner ,
Marianne Kreusch ,
Debora T. Pereira ,
Carmen Simioni ,
Giulia B. Costa  and
Paulo A. Horta
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Marthiellen R. de L. Felix
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Luz K.P. Osorio
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Luciane C. Ouriques
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Francine L. Farias-Soares
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Neusa Steiner
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Marianne Kreusch
Affiliation:
Scientific Initiation-PIBIC-CNPq, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Debora T. Pereira
Affiliation:
Scientific Initiation-PIBIC-CNPq, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Carmen Simioni
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Giulia B. Costa
Affiliation:
Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Paulo A. Horta
Affiliation:
Phycology Laboratory, Department of Botany, Federal University of Santa Catarina, 88040-900, Florianópolis, SC, Brazil
Fungyi Chow
Affiliation:
Department of Botany, Institute of Bioscience, University of São Paulo, 05508-090, São Paulo, SP, Brazil
Fernanda Ramlov
Affiliation:
Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Marcelo Maraschin
Affiliation:
Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Zenilda L. Bouzon
Affiliation:
Central Laboratory of Electron Microscopy, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
Éder C. Schmidt*
Affiliation:
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil
*
*Corresponding author. edcash@ccb.ufsc.br; eder.schmidt@pq.cnpq.br
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Abstract

The in vitro effect of cadmium (Cd) on apical segments of Pterocladiella capillacea was examined. Over a period of 7 days, the segments were cultivated with the combination of different salinities (25, 35, and 45 practical salinity units) and Cd concentrations, ranging from 0.17 to 0.70 ppm. The effects of Cd on growth rates and content of photosynthetic pigments were analyzed. In addition, metabolic profiling was performed, and samples were processed for microscopy. Serious damage to physiological performance and ultrastructure was observed under different combinations of Cd concentrations and salinity values. Elementary infrared spectroscopy revealed toxic effects registered on growth rate, photosynthetic pigments, chloroplast, and mitochondria organization, as well as changes in lipids and carbohydrates. These alterations in physiology and ultrastructure were, however, coupled to activation of such defense mechanisms as cell wall thickness, reduction of photosynthetic harvesting complex, and flavonoid. In conclusion, P. capillacea is especially sensitive to Cd stress when intermediate concentrations of this pollutant are associated with low salinity values. Such conditions resulted in metabolic compromise, reduction of primary productivity, i.e., photosynthesis, and carbohydrate accumulation in the form of starch granules. Taken together, these findings improve our understanding of the potential impact of this metal in the natural environment.

Keywords

Pterocladiella capillaceacadmiumsalinitymorphologymetabolic profilemetal toxicity
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 5 , October 2014 , pp. 1411 - 1424
Copyright
© Microscopy Society of America 2014 

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