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Differences in Chromatin Texture and Nuclear Fractal Dimension Between Hashimoto's and Lymphocytic Thyroiditis Lymphocytes

Published online by Cambridge University Press:  28 February 2019

Jasna Todorović ,
Marko Dinčić ,
Jelena Nešović Ostojić ,
Ivan Zaletel ,
Srdjan Lopičić ,
Duško Dundjerović ,
Svetislav Tatić ,
Sanjin Kovačević ,
Ivan Paunović  and
Nela Puškaš
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Jasna Todorović*
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Marko Dinčić*
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Jelena Nešović Ostojić
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Ivan Zaletel
Affiliation:
Faculty of Medicine, Institute of Histology and Embryology, University of Belgrade, Serbia
Srdjan Lopičić
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Duško Dundjerović
Affiliation:
Faculty of Medicine, Institute of Pathology, University of Belgrade, Serbia
Svetislav Tatić
Affiliation:
Faculty of Medicine, Institute of Pathology, University of Belgrade, Serbia
Sanjin Kovačević
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Ivan Paunović
Affiliation:
Faculty of Medicine, Center for Endocrine Surgery, Clinical Center of Serbia, University of Belgrade, Serbia
Nela Puškaš
Affiliation:
Faculty of Medicine, Institute of Histology and Embryology, University of Belgrade, Serbia
Ljiljana Marković
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
*
*Authors for correspondence: Todorović Jasna, E-mail: jasna.todorovic@med.bg.ac.rs; Dinčić Marko, E-mail: dincic89@yahoo.com
*Authors for correspondence: Todorović Jasna, E-mail: jasna.todorovic@med.bg.ac.rs; Dinčić Marko, E-mail: dincic89@yahoo.com
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Abstract

Previous evidence suggested that lymphocytic thyroiditis (LT) was a variant of Hashimoto's thyroiditis (HT), thus the aim of the current study is to quantify structural changes in histological specimens taken from HT and LT patients. A total of 600 images containing a single lymphocyte nucleus (300 nuclei per group) were obtained from 20 patients with HT and LT. In order to quantify changes in the nuclear architecture of investigated lymphocytes, the fractal dimension (FD) and some gray-level co-occurrence matrix texture parameters (angular second moment, inverse difference moment, contrast, entropy, and correlation) were calculated for each nucleus. A statistically significant difference in the FD of the “binary-outlined” nucleus and that of the corresponding “black-and-white” nucleus was detected between HT and LT lymphocyte nuclei. In addition, there was also a statistically significant difference in contrast and correlation between HT and LT lymphocyte nuclei. In conclusion, the results of this study suggested that there was a difference in structural complexity between investigated lymphocyte nuclei; additionally, LT lymphocytes possessed probably more complex texture and larger variations as well as more asymmetrical nuclei compared with HT lymphocytes. Accordingly, these findings indicate that LT is probably not a variant of HT; however, more complex studies are necessary to estimate differences between these types of thyroiditis.

Keywords

fractal dimensionGLCMHashimoto's thyroiditislymphocyte nucleilymphocytic thyroiditis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 25 , Issue 3 , June 2019 , pp. 762 - 768
Copyright
Copyright © Microscopy Society of America 2019 

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References

Anila, KR, Nayak, N & Jayasree, K (2016). Cytomorphologic spectrum of lymphocytic thyroiditis and correlation between cytological grading and biochemical parameters. J Cytol 33(3), 145–149.Google Scholar
Aozasa, K (1990). Hashimoto's thyroiditis as a risk factor of thyroid lymphoma. Acta Pathol Jpn 40(7), 459–468.Google Scholar
Bancaud, A, Huet, S, Daigle, N, Mozziconacci, J, Beaudouin, J & Ellenberg, J (2009). Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin. EMBO J 28(24), 3785–3798.Google Scholar
Bhatia, A, Rajwanshi, A, Dash, RJ, Mittal, BR & Saxena, AK (2007). Lymphocytic thyroiditis—is cytological grading significant? A correlation of grades with clinical, biochemical, ultrasonographic and radionuclide parameters. CytoJournal 4, 10.Google Scholar
Caturegli, P, De Remigis, A & Rose, NR (2014). Hashimoto thyroiditis: Clinical and diagnostic criteria. Autoimmun Rev 13(4–5), 391–397.Google Scholar
Chandanwale, SS, Gore, CR, Bamanikar, SA, Gupta, N & Gupta, K (2014). Cytomorphologic spectrum of Hashimoto's thyroiditis and its clinical correlation: A retrospective study of 52 patients. CytoJournal 11, 9.Google Scholar
Di Ieva, A, Grizzi, F, Jelinek, H, Pellionisz, AJ & Losa, GA (2014). Fractals in the neurosciences, part I: General principles and basic neurosciences. Neuroscientist 20(4), 403–417.Google Scholar
Di Ieva, A, Grizzi, F, Sherif, C, Matula, C & Tschabitscher, M (2011). Angioarchitectural heterogeneity in human glioblastoma multiforme: A fractal-based histopathological assessment. Microvasc Res 81(2), 222–230.Google Scholar
Fuseler, JW, Bedenbaugh, A, Yekkala, K & Baudino, TA (2010). Fractal and image analysis of the microvasculature in normal intestinal submucosa and intestinal polyps in Apc Min/+mice. Microsc Microanal 16(1), 73–79.Google Scholar
Gebejes, A & Huertas, R (2013). Texture characterization based on grey-level co-occurrence matrix. In Proceedings in Conference of Informatics and Management Sciences.Google Scholar
Guarda, LA & Baskin, HJ (1987). Inflammatory and lymphoid lesions of the thyroid gland. Cytopathology by fine-needle aspiration. Am J Clin Pathol 87(1), 14–22.Google Scholar
Gupta, S, Gupta, R, Singh, S, Gupta, K & Kudesia, M (2010). Nuclear morphometry and texture analysis of B-cell non-Hodgkin lymphoma: Utility in subclassification on cytosmears. Diagn Cytopathol 38(2), 94–103.Google Scholar
Haralick, RM & Shanmugam, K (1973). Textural features for image classification. IEEE Trans Syst Man Cybern 3(6), 610–621.Google Scholar
Lorthois, S & Cassot, F (2010). Fractal analysis of vascular networks: Insights from morphogenesis. J Theor Biol 262(4), 614–633.Google Scholar
Milošević, NT, Ristanović, D, Jelinek, HF & Rajković, K (2009). Quantitative analysis of dendritic morphology of the alpha and delta retinal ganglion cells in the rat: A cell classification study. J Theor Biol 259(1), 142–150.Google Scholar
Motoi, N & Ozawa, Y (2005). Malignant T-cell lymphoma of the thyroid gland associated with Hashimoto's thyroiditis. Pathol Int 55(7), 425–430.Google Scholar
Pantic, I, Harhaji-Trajkovic, L, Pantovic, A, Milosevic, NT & Trajkovic, V (2012 a). Changes in fractal dimension and lacunarity as early markers of UV-induced apoptosis. J Theor Biol 303, 87–92.Google Scholar
Pantic, I, Nesic, D, Stevanovic, D, Starcevic, V, Pantic, S & Trajkovic, V (2013). Effects of ghrelin on the structural complexity of exocrine pancreas tissue architecture. Microsc Microanal 19(3), 553–558.Google Scholar
Pantic, I, Pantic, S & Basta-Jovanovic, G (2012 b). Gray level co-occurrence matrix texture analysis of germinal center light zone lymphocyte nuclei: Physiology viewpoint with focus on apoptosis. Microsc Microanal 18(3), 470–475.Google Scholar
Park, SH, Shin, JW, Kang, YG, Hyun, J-S, Oh, MJ & Shin, J-W (2014). Texture analyses show synergetic effects of biomechanical and biochemical stimulation on mesenchymal stem cell differentiation into early phase osteoblasts. Microsc Microanal 20(1), 219–227.Google Scholar
Pearce, EN, Farwell, AP & Braverman, LE (2003). Thyroiditis. N Engl J Med 348(26), 2646–2655.Google Scholar
Righi, M, Giacomini, A, Lavazza, C, Sia, D, Carlo-Stella, C & Gianni, AM (2009). A computational approach to compare microvessel distributions in tumors following antiangiogenic treatments. Lab Invest 89(9), 1063.Google Scholar
Singer, PA (1991). Thyroiditis. Acute, subacute, and chronic. Med Clin North Am 75(1), 61–77.Google Scholar
Stankovic, M, Pantic, I, De Luka, SR, Puskas, N, Zaletel, I, Milutinovic-Smiljanic, S, Pantic, S & Trbovich, AM (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. J Microsc 261(3), 277–284.Google Scholar
Stuart, A (2011). The changing scene in Hashimoto's disease: A review. Med Hypotheses 77(3), 424–426.Google Scholar
Takasu, N & Yoshimura Noh, J (2008). Hashimoto's thyroiditis: TGAb, TPOAb, TRAb and recovery from hypothyroidism. Expert Rev Clin Immunol 4(2), 221–237.Google Scholar
Todorovic, J, Nesovic, JO, Opric, D, Dundjerovic, D, Bozic, V & Markovic, JL (2014). Is lymphocytic thyroiditis a unique type or merely a type of Hashimoto's thyroiditis? Minerva Med 105(4), 303–312.Google Scholar
Vakoc, BJ, Lanning, RM, Tyrrell, JA, Padera, TP, Bartlett, LA, Stylianopoulos, T, Munn, LL, Tearney, GJ, Fukumura, D & Jain, RK (2009). Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nat Med 15(10), 1219.Google Scholar
Vickerman, MB, Keith, PA, McKay, TL, Gedeon, DJ, Watanabe, M, Montano, M, Karunamuni, G, Kaiser, PK, Sears, JE & Ebrahem, Q (2009). VESGEN 2D: Automated, user-interactive software for quantification and mapping of angiogenic and lymphangiogenic trees and networks. Anat Rec 292(3), 320–332.Google Scholar
Walker, RF, Jackway, P & Longstaff, I (1995). Improving co-occurrence matrix feature discrimination. In DICTA'95, 3rd Conference on Digital Image Computing: Techniques and Application, pp. 643–648.Google Scholar
Woolf, PD (1980). Transient painless thyroiditis with hyperthyroidism: A variant of lymphocytic thyroiditis? Endocr Rev 1(4), 411–420.Google Scholar
Yang, X, Tridandapani, S, Beitler, JJ, Yu, DS, Yoshida, EJ, Curran, WJ & Liu, T (2012). Ultrasound GLCM texture analysis of radiation-induced parotid-gland injury in head-and-neck cancer radiotherapy: An in vivo study of late toxicity. Med Phys 39(9), 5732–5739.Google Scholar
Yogesan, K, Schulerud, H, Albregtsen, F & Danielsen, H (1998). Ultrastructural texture analysis as a diagnostic tool in mouse liver carcinogenesis. Ultrastruct Pathol 22(1), 27–37.Google Scholar