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CryoTEM with a Cold Field Emission Gun That Moves Structural Biology into a New Stage

Published online by Cambridge University Press:  05 August 2019

Takayuki Kato ,
Fumiaki Makino ,
Takanori Nakane ,
Naoya Terahara ,
Takeshi Kaneko ,
Yuko Shimizu ,
Sohei Motoki ,
Isamu Ishikawa ,
Koji Yonekura  and
Keiichi Namba
Takayuki Kato
Affiliation:
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Fumiaki Makino
Affiliation:
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Takanori Nakane
Affiliation:
MRC Laboratory of Molecular Biology, Cambridge, UK.
Naoya Terahara
Affiliation:
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Takeshi Kaneko
Affiliation:
JEOL, Akishima, Tokyo, Japan
Yuko Shimizu
Affiliation:
JEOL, Akishima, Tokyo, Japan
Sohei Motoki
Affiliation:
JEOL, Akishima, Tokyo, Japan
Isamu Ishikawa
Affiliation:
JEOL, Akishima, Tokyo, Japan
Koji Yonekura
Affiliation:
RIKEN SPring-8 Center, Sayo-cho, Hyogo, Japan.
Keiichi Namba*
Affiliation:
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan. RIKEN SPring-8 Center, Sayo-cho, Hyogo, Japan. RIKEN Center for Biosystems Dynamics Research, Suita, Osaka, Japan.
*
*Corresponding author: keiichi@fbs.osaka-u.ac.jp

Abstract

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Type
Advances in Cryo-EM Technology
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 998 - 999
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Li, X et al. , Nature Methods 10 (2013), p. 584.Google Scholar
[2]Scheres, SHW, Journal of Structural Biology 180 (2012), p. 519.Google Scholar
[3]Zivanov, J et al. , Elife 7 (2018), e42166.Google Scholar
[4]Kato, T et al. , EMDB ID: 6840.Google Scholar
[5]The authors acknowledge all the JEOL engineers and staffs for their efforts in the development of CRYO ARM, Haruaki Yanagisawa and Masahide Kikkawa for the plasmid of mouse apoferritin, and funding from AMED BINDS to TK and KN, AMED CiCLE to JEOL, and JSPS KAKENHI Grant number 25000013 to KN. Takayuki Kato, Fumiaki Makino and Takanori Nakane contributed equally.Google Scholar