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Comparative Cellular Localization of Sugar Residues in Bull (Bos taurus) and Donkey (Equus asinus) Testes Using Lectin Histochemistry

Published online by Cambridge University Press:  12 October 2021

Mahmoud S. Gewaily Open the ORCID record for Mahmoud S. Gewaily [Opens in a new window] ,
Mohamed Kassab ,
Asmaa Aboelnour ,
Essam A. Almadaly  and
Ahmed E. Noreldin Open the ORCID record for Ahmed E. Noreldin [Opens in a new window]
Mahmoud S. Gewaily*
Affiliation:
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516Kafrelsheikh, Egypt
Mohamed Kassab
Affiliation:
Department of Cytology and Histology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516Kafrelsheikh, Egypt
Asmaa Aboelnour
Affiliation:
Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, Damanhour22511, Egypt
Essam A. Almadaly
Affiliation:
Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, 33516, Kafrelsheikh, Egypt
Ahmed E. Noreldin
Affiliation:
Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, Damanhour22511, Egypt
*
*Corresponding author: Mahmoud Saad Gewaily, E-mail: drmahmoud_gewaily@yahoo.com, msgewaily@vet.kfs.edu.eg
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Abstract

Lectins are glycoproteins of a non-immune origin often used as histochemical reagents to study the distribution of glycoconjugates in different types of tissues. In this study, we performed a comparative cellular localization of sugar residues in bull and donkey testes using immunofluorescent lectin histochemistry. We inspected the cellular localization of the glycoconjugates within the testes using 11 biotin-labeled lectins (LCA, ConA, PNA, WGA, DBA, SBA, ECA, BPL, PTL-II, UEA-1, and PHA-E4) classified under six groups. Although the basic testicular structure in both species was similar, the cellular components showed different lectin localization patterns. The statistical analysis revealed no significant association between the intensity of labeling and different variables, including group and type of lectin and type of cell examined, at p < 0.05. However, a stronger response tended to occur in the donkey than in the bull testes (odds ratio: 1.3). These findings may be associated with the different cellular compositions of the glycoproteins and modification changes during spermatogenesis. Moreover, glycoconjugate profiling through lectin histochemistry can characterize some cell-type selective markers that will be helpful in studying bull and donkey spermatogenesis.

Keywords

bulldonkeyglycoconjugateslectin histochemistrytestis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 6 , December 2021 , pp. 1529 - 1538
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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