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Application of Phase Correlation to the Montage Synthesis and Three-Dimensional Reconstruction of Large Tissue Volumes from Confocal Laser Scanning Microscopy

Published online by Cambridge University Press:  10 March 2006

Mohamed-Adel Slamani ,
Andrzej Krol ,
Jacques Beaumont ,
Robert L. Price ,
Ioana L. Coman  and
Edward D. Lipson
Mohamed-Adel Slamani
Affiliation:
ITT Industries, Advanced Engineering and Sciences, Alexandria, VA 22315, USA
Andrzej Krol
Affiliation:
Department of Radiology, State University of New York Upstate Medical University, 750 E. Adams St., Syracuse, NY 13210, USA
Jacques Beaumont
Affiliation:
Department of Pharmacology, State University of New York Upstate Medical University, 750 E. Adams St., Syracuse, NY 13210, USA
Robert L. Price
Affiliation:
Department of Developmental Biology and Anatomy, School of Medicine, University of South Carolina, 6439 Garners Ferry Road, Columbia, SC 29209, USA
Ioana L. Coman
Affiliation:
Department of Mathematics and Computer Science, Ithaca College, 401C Williams Hall, Ithaca, NY 14850, USA
Edward D. Lipson
Affiliation:
Department of Physics, Syracuse University, Syracuse, NY 13244-1130, USA
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Abstract

We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce “tiles” of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.

Keywords

montage synthesisimage mosaickingphase correlationconfocal laser scanning microscopy
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 12 , Issue 2 , April 2006 , pp. 106 - 112
Copyright
© 2006 Microscopy Society of America

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