Skip to main content Accessibility help
×
Home

Transport Through the Secretory Pathway: Observations of Cargo and Peripheral Coat Proteins

Published online by Cambridge University Press:  02 July 2020

John F. Presley
Affiliation:
Cell Biology and Metabolism Branch, Bldg 18T, NICHD, National Institutes of Health, Bethesda, MD20892.
Nelson B. Cole
Affiliation:
Cell Biology and Metabolism Branch, Bldg 18T, NICHD, National Institutes of Health, Bethesda, MD20892.
Jennifer Lippincott-Schwartz
Affiliation:
Cell Biology and Metabolism Branch, Bldg 18T, NICHD, National Institutes of Health, Bethesda, MD20892.

Extract

We have used green fluorescent protein (GFP) chimeras to examine the dynamics of the early secretory pathway and the role of the peripheral coat protein COP I. To describe the overall properties of ER to Golgi transport we used the temperature sensitive viral glycoprotein, ts045 VSVG, tagged with GFP at its cytoplasmic tail. VSVG-GFP retained the temperature sensitive phenotype of its parent: it reversibly misfolded and was retained in the ER at 40°C. Upon shift to 32°C it was rapidly exported from the ER, moving as a synchronous pool into the Golgi complex and then to the cell surface. Using time-lapse imaging of living cells expressing VSVG-GFP we found that the carriers for ER to Golgi traffic are tubulovesicular pre-Golgi intermediates that move centrosomally to the Golgi at speeds of>1 μM2/sec and then fuse with the cis face of the Golgi complex. These movements are dependant on microtubules and the dynein/dynactin complex.

Type
Dynamics of Cellular Membrane Traffic
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below.

Usage data cannot currently be displayed.

Hostname: page-component-76cb886bbf-rm8z7 Total loading time: 0.368 Render date: 2021-01-22T23:53:18.644Z Query parameters: { "hasAccess": "0", "openAccess": "0", "isLogged": "0", "lang": "en" } Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false }

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Transport Through the Secretory Pathway: Observations of Cargo and Peripheral Coat Proteins
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Transport Through the Secretory Pathway: Observations of Cargo and Peripheral Coat Proteins
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Transport Through the Secretory Pathway: Observations of Cargo and Peripheral Coat Proteins
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *