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Structural Relationships Between Interstitial Cells of Cajal and Smooth Muscle Cells/Nerve Fibers in the Gastric Muscularis Mucosae of Chinese Giant Salamander

Published online by Cambridge University Press:  23 December 2020

Hang Yu
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Yangquan Liu
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Meng Chu
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Yu Si
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Yaqiong Ye
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Tingting Ge
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Haiquan Zhao
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China
Hui Zhang*
Affiliation:
College of Life Science and Engineering, Foshan University, Foshan528231, China College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang330045, China
*
*Author for correspondence: Hui Zhang, E-mail: zhanghui429@hotmail.com

Abstract

Interstitial cells of Cajal (ICC) play an essential role in the motility of the gastrointestinal tract, and they have been identified in many laboratory animals and in humans. However, the information of ICC in lower animals is still very limited. In the present study, ICC were identified in the gastric muscularis mucosae of an amphibian—the Chinese giant salamander, by c-Kit immunohistochemistry and transmission electron microscopy. ICC showed c-Kit immunoreactivity and had spindle-shaped cell bodies and 1–2 long processes. ICC were located between smooth muscle cells (SMC) in gastric muscularis mucosae. Ultrastructurally, ICC appeared as polygon-, spindle-, and awl-shaped with long cytoplasmic prolongations between SMC. ICC had distinctive characteristics, such as nuclei with peripheral electron-dense heterochromatin, caveolae, and abundant intracytoplasmatic vacuoles, mitochondria, and rough endoplasmic reticula. Moreover, lamellar bodies and two types of condensed granules were observed in the cytoplasm of ICC. Notably, ICC establish close contacts with each other. Moreover, ICC establish gap junctions with SMC. In addition, ICC were frequently observed close to nerve fibers. In summary, the present study demonstrated the presence of ICC in the gastric muscularis mucosae of the Chinese giant salamander.

Type
Micrographia
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of the Microscopy Society of America

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