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Measurements Validating the Confocal Scanning Laser Holography Microscope

Published online by Cambridge University Press:  13 July 2011

Peter B. Jacquemin*
University of Victoria, Department of Mechanical Engineering, 3800 Finnerty Road, BC V8P 5C2, Canada
Rodney A. Herring
University of Victoria, Department of Mechanical Engineering, 3800 Finnerty Road, BC V8P 5C2, Canada
Corresponding author. E-mail:
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A confocal scanning laser holography (CSLH) microscope that uniquely combines the concepts of confocal microscopy with holography has been validated for making nonintrusive, full three-dimensional (3D) intensity and phase measurements of objects from a single viewpoint of observation without loss of object information. The phase measurements have been used to determine the 3D refractive indices of a point source heated silicone oil. The refractive indices are converted to 3D temperature measurements, which are useful for heat transfer studies. An important feature of CSLH is its nonintrusive 3D scanning method, which enables its application to the study of Marangoni convection in microgravity with minimal operational vibrations affecting the motion of fluid in the specimen.

Technology and Software Development Light and Confocal Microscopy
Copyright © Microscopy Society of America 2011

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