Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Home
  • Home
Hostname: page-component-5bf98f6d76-94zm5 Total loading time: 0.224 Render date: 2021-04-20T13:05:30.222Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }
EnglishFrançais
Microscopy and Microanalysis Microscopy and Microanalysis

Article contents

An Integrated System for Transmission Electron Microscopy.

Published online by Cambridge University Press:  02 July 2020

B. Carragher ,
N. Kisseberth ,
D. Kriegman ,
R.A. Milligan ,
C.S. Potter ,
J. Pulokas  and
A. Reilein
B. Carragher
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
N. Kisseberth
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
D. Kriegman
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
R.A. Milligan
Affiliation:
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA92037
C.S. Potter
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
J. Pulokas
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
A. Reilein
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
Get access
Rights & Permissions[Opens in a new window]

Extract

Macromolecular microscopy is becoming an increasingly important tool for structural biology. The development of improved capabilities for three-dimensional electron microscopy is critical for optimal progress in emerging integrative research in molecular cell biology. These techniques currently suffer from several severe disadvantages related to the tremendous time and effort required to acquire and analyze the data.

For several years we have been developing software for automated and intelligent acquisition of transmission electron micrographs [1,2,3]. Our overall goal is to develop a system for rapid and routine structure determination of macromolecular assemblies from specimens preserved in vitreous ice. Ultimately we plan to develop an integrated system that can produce an electron density map of a structure within a few hours of inserting a specimen into the electron microscope. With this goal in mind it is essential that the images be acquired using a digital camera rather than film.

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 280 - 281
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below.
If you should have access and can't see this content please contact technical support.

References

1.Kisseberth, N., et al. (1998) JSB 120, 309–319.Google Scholar
2.Potter, C.S., et al. (1999) Ultramicroscopy, 77, 153–161.CrossRefGoogle Scholar
3.Pulokas, J., et al. (1999) JSB. 128, 250–256.Google Scholar
4.Ermantraut, E, et al. (1998) Ultramicroscopy 74 75–81.CrossRefGoogle Scholar
5.Koster, A. J. and de Ruijter, W. J. (1992) Ultramicroscopy, 40, 89–107.CrossRefGoogle Scholar
6. Support is provided by the NSF (9730056 and 9904547) and the IBM Shared University Research program.Google Scholar

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 0
Total number of PDF views: 4 *
View data table for this chart

* Views captured on Cambridge Core between 02nd July 2020 - 20th April 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

An Integrated System for Transmission Electron Microscopy.
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

An Integrated System for Transmission Electron Microscopy.
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

An Integrated System for Transmission Electron Microscopy.
Available formats
×
×

Reply to: Submit a response

Your details

Conflicting interests

Do you have any conflicting interests? *