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A new record of the Kumamoto oyster Crassostrea sikamea in the Seto Inland Sea, Japan

Published online by Cambridge University Press:  13 February 2013

Masami Hamaguchi ,
Hiromori Shimabukuro ,
Masako Kawane  and
Tomoki Hamaguchi
Masami Hamaguchi*
Affiliation:
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Hiromori Shimabukuro
Affiliation:
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Masako Kawane
Affiliation:
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Tomoki Hamaguchi
Affiliation:
Itsukaichi Minami Junior High school, Kairouen 4-2-21, Hiroshima 731-5135, Japan
*
Correspondence should be addressed to: M. Hamaguchi, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan email:masami@fra.affrc.go.jp
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Abstract

We collected 138 Kumamoto like oyster specimens in and around the Nakatsu tidal flat to monitor species diversity. The mitochondrial large subunit of ribosomal DNA (LSrDNA) and the nuclear ribosomal internally transcribed spacer-1 (ITS-1) region were amplified by polymerase chain reaction and restriction fragment length polymorphism analysis, leading to the discovery of the Kumamoto oyster Crassostrea sikamea in the Nakatsu tidal flat. A more accurate species identification using the nucleotide sequences for cytochrome c oxidase subunit I and ITS-1 confirmed the presence of the oysters. These results provide the first evidence of C. sikamea in the Seto Inland Sea of Japan.

Keywords

Crassostrea sikameapolymerase chain reaction–restriction fragment length polymorphismnucleotide sequenceSeto Inland SeaJapan
Type
Research Article
Information
Marine Biodiversity Records , Volume 6 , 2013 , e16
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013

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