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A new record of the Kumamoto oyster Crassostrea sikamea in the Seto Inland Sea, Japan

  • Masami Hamaguchi (a1), Hiromori Shimabukuro (a1), Masako Kawane (a1) and Tomoki Hamaguchi (a2)

Abstract

We collected 138 Kumamoto like oyster specimens in and around the Nakatsu tidal flat to monitor species diversity. The mitochondrial large subunit of ribosomal DNA (LSrDNA) and the nuclear ribosomal internally transcribed spacer-1 (ITS-1) region were amplified by polymerase chain reaction and restriction fragment length polymorphism analysis, leading to the discovery of the Kumamoto oyster Crassostrea sikamea in the Nakatsu tidal flat. A more accurate species identification using the nucleotide sequences for cytochrome c oxidase subunit I and ITS-1 confirmed the presence of the oysters. These results provide the first evidence of C. sikamea in the Seto Inland Sea of Japan.

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Corresponding author

Correspondence should be addressed to: M. Hamaguchi, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan email:masami@fra.affrc.go.jp

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Keywords

A new record of the Kumamoto oyster Crassostrea sikamea in the Seto Inland Sea, Japan

  • Masami Hamaguchi (a1), Hiromori Shimabukuro (a1), Masako Kawane (a1) and Tomoki Hamaguchi (a2)

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