Skip to main content Accessibility help
×
Home

Contents:

Information:

  • Access
  • Cited by 2

Actions:

      • Send article to Kindle

        To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

        Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

        Find out more about the Kindle Personal Document Service.

        First record of Zenion hololepis (Zenionidae) in Portuguese continental waters: the northernmost occurrence in the eastern Atlantic
        Available formats
        ×

        Send article to Dropbox

        To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

        First record of Zenion hololepis (Zenionidae) in Portuguese continental waters: the northernmost occurrence in the eastern Atlantic
        Available formats
        ×

        Send article to Google Drive

        To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

        First record of Zenion hololepis (Zenionidae) in Portuguese continental waters: the northernmost occurrence in the eastern Atlantic
        Available formats
        ×
Export citation

Abstract

The occurrence of Zenion hololepis in Portuguese continental waters is reported for the first time. A total of five specimens were collected in two separate fisheries research surveys, in 2008 and 2009. Analyses of morphometric and meristic characteristics confirmed all five specimens as Z. hololepis, while DNA barcode sequence data delivered a genus-level assignment. The records here described constitute a new extended northern limit for the distribution of Z. hololepis in the eastern Atlantic and the first DNA barcode data for this species.

The species Zenion hololepis (Goode & Bean, 1896) belongs to the relatively small family Zenionidae, which comprises only three genera and seven species (Heemstra, Reference Heemstra, Smith and Heemstra1986), although the number of species and genera within this family is not consensual (see discussion in Heemstra, Reference Heemstra, Smith and Heemstra1986). Members of this family are typically found over mud or sand–mud bottom and usually at depths between 180 and 700 m (Heemstra, Reference Heemstra, Smith and Heemstra1986). Information about the species Z. hololepis is relatively scarce, both in dedicated databases such as FishBase (www.fishbase.org) and in the scientific literature. This species seems to have a wide distribution (Figure 1), including the western and north-western Atlantic (Scott & Scott, Reference Scott and Scott1988; Claro, Reference Claro and Claro1994) and the western central Pacific (Heemstra, Reference Heemstra, Carpenter and Niem1999). FishBase indicates the occurrence of Z. hololepis also in the western Indian Ocean and South China Sea (Froese & Pauly, Reference Froese and Pauly2010). In the eastern Atlantic, Z. hololepis occurs from Cape Timiris (Mauritania) to South Africa (Quéro, Reference Quéro, Maurin and Quéro1981).

Fig. 1. AquaMaps for Zenion hololepis (Kaschner et al., Reference Kaschner, Ready, Agbayani, Rius, Kesner-Reyes, Eastwood, South, Kullander, Rees, Close, Watson, Pauly and Froese2007), upon modification. Dots represent the occurrence of the species as reported in FishBase (www.fishbase.org). Squares show the locations where Z. hololepis specimens were caught along the Portuguese coasts.

On the course of two separate fisheries research campaigns of IPIMAR (Portuguese fisheries research agency) five uncommon specimens, subsequently identified as Z. hololepis, were collected. The specimens were caught off the south-west coast of Portugal (Figure 1), by the research vessel ‘Noruega’, in September 2008 (collection of two specimens at the geographical coordinates: 38°0.2′N 09°7.2′W 37°57.4′N 09°08.6′W) and June 2009 (sampling of three specimens at the geographical coordinates: 37°57.4′N 09°08.6′W 37°21.40′N 09°10.2′W and 37°24.9′N 09°09.6′W). Fish were captured using a bottom trawl operating at a depth between 340 and 361 m. The specimens were carefully examined for diagnostic morphological features, following description provided by Heemstra (Reference Heemstra, Smith and Heemstra1986). According to this author, Z. hololepis displays an elongate body and a head length 2.3 to 2.7 times the standard length, the latter one being the measurement from the most anterior tip of the body to the posterior end of the vertebral column. The upper jaw is extremely protractible. The dorsal fin has 6 to 7 spines and 25–28 rays, with the front edge of the second spine distinctly serrate. Pectoral fins show 15–17 rays, while the pelvic fins are characterized by serrate spine and 6 branched rays. The main characteristic that distinguishes this species from the congeneric Zenion leptolepis is the anal fin, with 23–28 rays in the former species, and 28–32 rays in the latter one.

The specimens identified as Zenion hololepis (Figure 2) were photographed and preserved in 70% ethanol for storage at the National Museum of Natural History in Lisbon. Table 1 reports the range of meristic and morphometric characteristics and body proportions of the Z. hololepis specimens under consideration. Among the five specimens, the total length ranged from 79 to 134 mm and their total weight from 18.9 to 51.5 g.

Fig. 2. One of the five specimens of Zenion hololepsis (134 mm total length and 51.49 g) caught in Portuguese continental waters (north-eastern Atlantic).

Table 1. Range of meristic characteristics (number), morphometric characteristics (mm) and body proportions of Zenion hololepis specimens caught off the Portuguese continental coast.

L S, percentage of standard length; L H, percentage of head length. Roman and Arabic numerals represent hard and soft fin rays, respectively.

To further characterize the specimens and substantiate the taxonomic identification, we complemented our observations using genetic tags for species identification, i.e. DNA barcodes (Hebert et al., Reference Hebert, Cywinska, Ball and deWaard2003). Namely, we analysed the sequence variation of a 652 base pair (bp) fragment of the mitochondrial DNA gene cytochrome c oxidase I (5′-COI).

A sample of muscle tissue of each individual was collected and preserved in 96% ethanol for molecular analyses. Total genomic DNA was extracted from white muscle tissue, and DNA barcode regions amplified and sequenced following published protocols (Ivanova et al., Reference Ivanova, Zemlak, Hanner and Hebert2007; Rock et al., Reference Rock, Costa, Walker, North, Hutchinson and Carvalho2008).

After editing the bidirectional sequences, we obtained five 652 bp DNA barcodes, which were deposited in the GenBank® with the following accession numbers: JF718831–JF718835. Details of the respective PCR primers, sequences and trace files are publicly available on the Barcode of Life Data Systems (BOLD; www.barcodinglife.org, under the project titled ‘First record of Zenion hololepis in Portuguese continental waters [MLFPZ]’. Among all five specimens, DNA barcodes differed at most by two nucleotides, and consequently the average conspecific divergence was very low [0.2% Kimura-2-parameter (K2P)]. We submitted Z. hololepis DNA barcodes to GenBank's BLAST® (http://www.ncbi.nlm.nih.gov/BLAST) and to BOLD's (Ratnasingham & Hebert, Reference Ratnasingham and Hebert2007) identification engine, in search for matching sequences. No complete matches were found, but the nearest match (90.7% identity) belonged to a homologous COI sequence of the congeneric species Zenion japonicum (GenBank® Accession AP004434). Zenion japonicum is the only species of Zenion for which there is any genetic data available in GenBank® and BOLD. Comparison of the aligned nucleotide sequences of the two Zenion species revealed an average divergence of 10.1% (K2P), which is within the range found in DNA barcodes of congeneric fish species (Ward et al., Reference Ward, Zemlak, Innes, Last and Hebert2005). Hence, this nucleotide genetic distance provides evidence of the existence of at least two different species of the genus Zenion. Additionally, the corresponding amino acid sequences of the two Zenion species were identical, confirming their close evolutionary relatedness. The sequences here reported are the first published DNA barcodes for Z. hololepis, providing reference genetic tags for comparison with specimens of Zenion spp. collected elsewhere in the future.

There are no records in the literature of the occurrence of Z. hololepis in the north-eastern Atlantic Ocean, nor in the IPIMAR's fisheries surveys database, which spans several decades of regular research campaigns along Portuguese waters.

There have been various recent reports on expansions of the northern limit of warm water species towards the temperate north-eastern Atlantic, including new occurrences along the Portuguese coast (Azevedo et al., Reference Azevedo, Raposeiro and Rodrigues2004; Lima et al., Reference Lima, Ribeiro, Queiroz, Hawkins and Santos2007; Baňón & Sande, Reference Baňón and Sande2008; Borges et al., Reference Borges, Valente, Palma and Nunes2010).

Based on current evidence, it is not possible to conclude if this new observation of Z. hololepis in areas previously unrecorded is a fortuitous observation or a more permanent expansion of its northern distribution range. However, the fact that this species was captured on two independent occasions separated by 9 months favours the latter possibility.

Acknowledgements

We thank Pedro da Conceição for providing us with specimens and J. Olney, R. Lord, T. Kwak, W. Starnes, S. Ross, J. Caruso, J. Moore and A. Gosztonyi for help in Z. hololepis identification. This work was partially supported by Fundação para a Ciência e a Tecnologia (FCT) research grant METAFISHCODE PTDC/MAR/101795/2008. The work of M. Landi was supported by an FCT fellowship reference SFRH/BPD/45246/2008.

References

Azevedo, J.M.N., Raposeiro, P.M. and Rodrigues, L. (2004) First records of Fistularia petimba and Diodon eydouxii for the Azores, with notes on the occurrence of three additional species. Journal of Fish Biology 65, 11801184.
Baňón, R. and Sande, C. (2008) First record of the red cornetfish Fistularia petimba (Syngnathiformes: Fistularidae) in Galician waters: a northernmost occurrence in the eastern Atlantic. Journal of Applied Ichthyology 24, 106107.
Borges, L.M.S., Valente, A.A., Palma, P. and Nunes, L. (2010) Changes in the wood boring community in the Tagus Estuary: a case study. Marine Biodiversity Records 3, 17.
Claro, R. (1994) Características generales de la ictiofauna. In Claro, R. (ed.) Ecología de los peces marinos de Cuba. Instituto de Oceanología Academia de Ciencias de Cuba and Centro de Investigaciones de Quintana Roo, pp. 5570.
Froese, R. and Pauly, D. (eds) (2010) FishBase. World Wide Web electronic publication www.fishbase.org, version (11/2010).
Hebert, P.D.N., Cywinska, A., Ball, S.L. and deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B 270, 313321.
Heemstra, P.C. (1986) Zeniontidae. In Smith, M.M. and Heemstra, P.C. (eds) Smiths' Sea Fishes Berlin: Springer-Verlag, p. 441.
Heemstra, P.C. (1999) Macrurocyttidae. Dwarf dories. In Carpenter, K.E. and Niem, V.H. (eds) FAO species identification guide for fishery purposes. The living marine resources of the Western Central Pacific. Volume 4: Bony fishes part 2 (Mugilidae to Carangidae) Rome: FAO, pp. 2258.
Ivanova, N.V., Zemlak, T.S., Hanner, R.H. and Hebert, P.D.N. (2007) Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes 7, 544548.
Kaschner, K., Ready, J.S., Agbayani, E., Rius, J., Kesner-Reyes, K., Eastwood, P.D., South, A.B., Kullander, S.O., Rees, T., Close, C.H., Watson, R., Pauly, D. and Froese, R. (2007) AquaMaps: predicted range maps for aquatic species. World wide web electronic publication, www.aquamaps.org, Version 08/2010.
Lima, F.P., Ribeiro, P.A., Queiroz, N., Hawkins, S.J. and Santos, A.M. (2007) Do distributional shifts of northern and southern species of algae match the warming pattern? Global Change Biology 13, 25922604.
Quéro, J.C. (1981) Zeiformes. In Maurin, C. and Quéro, J.C. (eds) Poissons dês côtes nord-ouest africaines (campagnes de la Thalassa 1962, 1968, 1971 et 1973). Saccopharyngiformes, Anguilliformes, Beryciformes, Zeiformes, Lampridiformes et Gasterosteiformes. Revue des Travaux de l'Institut des Peches Maritime 45, 5–71.
Ratnasingham, S. and Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes 7, 355364.
Rock, J., Costa, F.O., Walker, D.I., North, A.W., Hutchinson, W.F. and Carvalho, G.R. (2008) DNA barcodes of fish of the Scotia Sea, Antarctica indicate priority groups for taxonomic and systematics focus. Antarctic Science 20, 253262.
Scott, W.B. and Scott, M.G. (1988) Atlantic fishes of Canada. Canadian Bulletin of Fisheries and Aquatic Sciences 219, 1731.
Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R. and Hebert, P.D.N. (2005) DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society, Series B 360, 18471857.