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X-ray microscopy of living multicellular organisms with the Prague Asterix Iodine Laser System

  • T. DESAI (a1), D. BATANI (a1), A. BERNARDINELLO (a1), G. POLETTI (a2), F. ORSINI (a2), J. ULLSCHMIED (a3), L. JUHA (a3), J. SKALA (a3), B. KRALIKOVA (a3), E. KROUSKY (a3), M. PFEIFER (a3), C. KADLEC (a3), T. MOCEK (a3), A. PRÄG (a3), O. RENNER (a3), F. COTELLI (a4), C. LORA LAMIA (a4) and A. ZULLINI (a5)...


Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were λ = 1.314 μm and τ (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I ≥ 1014 W/cm2. Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.


Corresponding author

Address correspondence and reprint requests to: Tara Desai, Dipartimento di Fisica “G. Occhialini” and INFM, Università di Milano Bicocca, Piazza della Scienza 3, 20126 Milano, Italy. E-mail:


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Bargmann, C.I. & Avery, L. (1995). Laser Killing of cells in Caenorhabditis elegans. Methods Cell Biol. 48, 225249.
Batani, D., Masini, A., Lora Lamia Donin, C., Cotelli, F., Previdi, F., Milani, M., Faral, B., Conte, E., Moret M., Poletti, G., &Pozzi, A. (1998). Characterisation of Saccharomyces cerevisiae yeast cells. Phys. Medica 15, 151157.
Batani, D., Botto, C., Bortoloto, F., Masini, A., Bernardinello, A., Moret, M., Milani, M., Eidmann, K., Poletti, G., Cotelli, F., Donin, C.L.L., Piccoli, S., Stead, A., Ford, T., Marranca, A., Flora, F., Palladino, L. & Reale, L. (2000). Contact X-ray microscopy using the Astrix laser source. Phys. Medica 16, 4955.
Bortolotto, F., Batani, D., Masini, A., Previdi, F., Rebonato, L. & Tureu, E. (2000). Study of a X-ray laser-plasma source for radiobiological experiments, microdosimetry analysis and plasma characterisation. Euro. Phys. J. D 11, 309.
Cox, G.N., Staprans, S. & Edgar, R.S. (1981). The cuticle of Caenorhabditis elegans. Dev. Biol. 86, 456470.
Fletcher, J., Cotton, R.A. & Webb, C. (1992). Soft x-ray contact microscopy using laser generated plasma sources. Proc. SPIE 1741, 142153.
Ford, T.W., Stead, A.D. & Cotton R.A. (1991). Soft x-ray contact microscopy of biological materials. Electron Microsc. Rev. 4, 269292.
Ford, T.W., Page, A.M., Foster, G.F. & Stead, A.D. (1992). Effects of soft x-ray irradiation on cell ultrastructure. Proc. SPIE 1741, 325332.
Foster, G.F., Buckley, C.J., Bennett, P.M. & Burge, R.E. (1992). Investigation of radiation damage to biological specimens at water window wavelengths. Rev. Scient. Instr. 63, 599600.
Henke, B.L. & Jaanimagi, P.A. (1985). Two-channel, elliptical analyzer spectrograph for absolute, time-resolving time-integrating spectrometry of pulsed x-ray sources in the 100–10000-eV region. Rev. Sci. Instrum. 56, 15371552.
Henke, B.L. (1986). Low-energy x-ray response of photographic films. Mathematical models. J. Opt. Soc. Am. B 3, 11, 15401546.
Jungwirth, K., Cejnarova, A., Juha, L., Kralikova, B., Krasa, J., Drousky, E., Krupickova, P., Laska, L., Masek, K., Mocek, T., Pfeifer, M., Präg, A., Renner, O., Rohlena, K., Rus, B., Skala, J., Straka, P. & Ullschmied, J. (2001). The Prague Astrix Laser System. Phys. Plasmas 8, 24952501.
Kondo, K. & Tomie, T. (1994). Optimization of a laser-plasma X-ray source for contact X-ray microscopy. J. Appl. Phys. 75, 37983805.
Lee, R.W. (1990). User Manual for RATION. Livermore, CA: Lawrence Livermore National Laboratory.
Masini, A., Batani, D., Previdi, F., Conti, A., Pisani, F., Botto, C., Bortolotto, F., Torsiello, F., Tureu, E., Allot, R., Lisi, N., Milani, M., Costato, M., Pozzi, A. & Koenig, M. (1997). X-ray irradiation of yeast cells. Proc. SPIE 3157, 203217.
Nobel Foundation. (2002). The Nobel Prize in Physiology or Medicine. Press Release, October 7.
Stead, A.D., Cotton, R.A., Page, A.M., Dooley, M. & Ford, T.W. (1992). Visualization of the effects of electron microscopy fixatives on the structure of hydrated epidermal hairs of tomato (Lycopersicum peruvianum) as revealed by soft X-ray contact microscopy. Proc. SPIE 1741, 351362.
Stead, A.D., Page, A.M., Cotton, R.A., Neely, D., Bagby, R., Miura, E., Tomie, T., Shimizue, S., Majima, T., Anastasi, P.A.F. & Ford, T.W. (1995b). Modifications for soft X-ray contact microscopy-quantification of carbon density discrimination and stereo imaging. Proc. SPIE 2523, 202211.
Stead, A.D., Page, A.M. & Ford, T.W. (1995a). The prospects for soft X-ray contact microscopy using laser plasmas as a X-ray source. Proc. SPIE 2523, 4050.
Rockett, P.D., Bird, C.R., Hailey, C.J., Sullivan, D., Brown, D.B. & Burkhalter, P.G. (1985). X-ray calibration of Kodak Direct Exposure film. Appl. Opt. 24, 25362542.
Tallents, G.J. (1987). X-ray spectroscopy of tokamaks. JET Report No. JET-P(87) 56.



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