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X-ray microscopy of living multicellular organisms with the Prague Asterix Iodine Laser System

  • T. DESAI (a1), D. BATANI (a1), A. BERNARDINELLO (a1), G. POLETTI (a2), F. ORSINI (a2), J. ULLSCHMIED (a3), L. JUHA (a3), J. SKALA (a3), B. KRALIKOVA (a3), E. KROUSKY (a3), M. PFEIFER (a3), C. KADLEC (a3), T. MOCEK (a3), A. PRÄG (a3), O. RENNER (a3), F. COTELLI (a4), C. LORA LAMIA (a4) and A. ZULLINI (a5)...

Abstract

Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were λ = 1.314 μm and τ (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I ≥ 1014 W/cm2. Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.

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Corresponding author

Address correspondence and reprint requests to: Tara Desai, Dipartimento di Fisica “G. Occhialini” and INFM, Università di Milano Bicocca, Piazza della Scienza 3, 20126 Milano, Italy. E-mail: tara.desai@mib.infn.it

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