The feeding habits of the Rio skate, Rioraja agassizi, from off Uruguay and north Argentina were evaluated using a multiple-hypothesis modelling approach. Relationships between number of preys and sex, maturity stage, body size, season and region were assessed by building generalized linear models. Rioraja agassizi fed mainly upon crustaceans (shrimps, crabs and amphipods) and teleosts, but also upon isopods, cumaceans, lancelets and polychaetes. Ontogenetic diet shifts were found: small R. agassizi consumed amphipods, cumaceans and isopods and large individuals on shrimps, crabs and teleosts. The consumption of crustaceans (amphipods, shrimps, crabs, isopods and cumaceans) was higher in the northern (34°–38°S) than in the southern area (38°–41°S). Rioraja agassizi consumed more teleosts in the cold season and preyed more on lancelets in the warm season. Prey size increased with increasing body size of R. agassizi, but large individuals also consumed small prey. Ontogenetic shifts may be related to body size rather than other life-history traits. Rioraja agassizi adapts its feeding habits in response to regional and seasonal changes.