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Trophic importance of meiofauna to polychaetes in a seagrass (Zostera marina) bed as traced by stable isotopes

  • Sun_Yong Ha (a1), Won-Ki Min (a2), Dong-Sung Kim (a2) and Kyung-Hoon Shin (a1)


Stable carbon and nitrogen isotope ratios of benthic invertebrates and their potential food sources, such as suspended particulate organic matter (POM), benthic microalgae, attached algae and seagrass, were identified in Dong-dae Bay during the winter. The carbon stable isotope ratios demonstrate that filter feeders, such as oysters (−19.5 ± 1.0‰), use benthic microalgae (−21.2 ± 0.2‰) as a major food, and polychaetes such as Glycera spp. (−14.0 ± 0.6 ‰) preferentially use meiofauna, such as nematodes (−14.0 ± 0.4‰) and copepods (−13.3 ± 1.0‰). These meiofauna may feed on mixed resources (including bacteria) with the isotope ratios between benthic microalgae (−21.2 ± 0.2‰) and seagrass (−9.3 ± 01.0‰). These findings are consistent with the trophic enrichment in the nitrogen isotope ratios (by 3–4‰) between consumers and food sources. Moreover, the results of the MixSIR model based on the observed isotope ratios suggest a large seagrass contribution to the food sources of benthic organisms such as meiofauna (~53.7–62.6%) and macrobenthos (~41.1–68%) through the food web. This model additionally suggests a relatively large contribution of benthic microalgae to the food sources of filter feeders (i.e. 26.4% for oysters).


Corresponding author

Correspondence should be addressed to: K.-H. Shin, Department of Environmental Marine Sciences, Hanyang University, 1271-3 dong, Sangnok-gu, Ansan, Kyeonggi-do, 425-791, Republic of Korea email:


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Aberle, N. and Malzahn, A.M. (2007) Interspecific and nutrient-dependent variations in stable isotope fractionation: experimental studies simulating pelagic multitrophic systems. Oceologia 154, 291303.
Anesio, A.M., Abreu, P.C. and Biddanda, B.A. (2003) The role of free and attached microorganisms in the decomposition of estuarine macrophyte detritus. Estuarine, Coastal and Shelf Science 56, 197201.
Barillé, L., Prou, J., Héral, M. and Razet, D. (1997) Effects of high natural seaton concentrations on the feeding, selection, and absorption of the oyster Crassostrea gigas (Thunberg). Journal of Experimental Marine Biology and Ecology 212, 149172.
Bell, S.S., Walters, K. and Kern, J.C. (1984) Meiofauna from seagrass habitats: a review and prospectus for future research. Estuaries 7, 331338.
Blanchard, G.F., Sauriau, P.G., Cariou-Le, G., Gouleau, D., Garet, M.J., and Olivier, F. (1997) Kinetics of tidal resuspension of microbiota: testing the effects of sediment cohesiveness and bioturbation using flume experiments. Marine Ecology Progress Series 151, 1725.
Bunn, S.E., Barton, D.R., Hynes, N., Power, G. and Pope, M.A. (1989) Stable isotope analysis of carbon flow in a tundra river ecosystem. Canadian Journal of Fisheries and Aquatic Sciences 46, 17691775.
Bunn, S.E., Loneragan, N.R. and Kempser, M.A. (1995) Effects of acid washing on stable ratios of C and N in penaeid shrimp and seagrass: implications for food-web studies using multiple stable isotopes. Limnology and Oceanography 40, 622625.
Bustamante, R. and Branch, G. (1996) The dependence of intertidal consumers on kelp-derived organic matter on the west coast of South Africa. Journal of Experimental Marine Biology and Ecology 196, 128.
Coffin, R.B., Fry, B. and Wright, R.T. (1989) Carbon isotopic compositions of estuarine bacteria. Limnology and Oceanography 34, 13051310.
Congie, B. and Barillé, L. (1999) Does bivalve mucus favour the growth of their main food source, microalgae? Oceanologica Acta 22, 441450.
Congi, B., Barillé, L., Massé, G. and Beninger, P.G. (2003) Selection and processing of large suspended algae in the oyster Crassostrea gigas. Marine Ecology Progress Series 250, 145152.
Couch, C.A. (1989) Carbon and nitrogen stable isotopes of meiobenthos and their food resources. Estuarine, Coastal and Shelf Science 28, 433441.
Crosby, M.P., Langdon, C.J. and Newell, R.I.E. (1989) Importance of refractory plant material to the carbon budget of the oyster Crassostrea virginica. Marine Biolgoy 100, 343352.
Danovaro, R. (1996) Detritus–bacteria–meiofauna interactions in a seagrass bed (Posidonia oceanica) of the NW Mediterranean. Marine Biolgy 127, 113.
Danovaro, R. and Fabiano, M. (1995) Seasonal and interannual variation of benthic bacteria in a seagrass bed of the Mediterranean Sea: relationship with labile organic compounds and other environmental factors. Aquatic Microbial Ecology 9, 1726.
Danovaro, R. and Gambi, C. (2002) Biodiversity and trophic structure of nematode assemblages in seagrass systems: evidence for a coupling with changes in food availability. Marine Biology 141, 667677.
Danovaro, R., Fabiano, M. and Boyer, M. (1994) Seasonal changes of benthic bacteria in a seagrass bed (Posidonia oceanica) of the Ligurian Sea in relation to origin composition and fate of the sediment organic matter. Marine Biology 119, 489500.
Decottignies, P., Beninger, P.G., Rincé, Y. and Riera, P. (2007) Trophic interactions between two introduced suspension-feeders, Crepidula fornicate and Crassostrea gigas, are influenced by seasonal effects and qualitative selection capacity. Journal of Experimental Marine Biology and Ecology 342, 231241.
Deegan, L.A. and Garritt, R.H. (1997) Evidence for spatial variability in estuarine food webs. Marine Ecology Progress Series 147, 3147.
De Jonge, V.N. and van Beusekom, J.E.E. (1992) Contribution of resuspended microphytobenthos to total phytoplankton in the Ems estuary. Limnology and Oceanography 40, 766778.
DeNiro, M.J. and Epstein, S. (1978) Influence of diet on the distribution of carbon isotopes in animals. Geochimica et Cosmochimica Acta 42, 495506.
DeNiro, M.J. and Epstein, S. (1981) Influence of diet on the distribution of nitrogen isotopes in animals. Geochimica et Cosmochimica Acta 45, 341351.
De Troch, M., Gurdebeke, S., Fiers, F. and Vincx, M. (2001) Zonation and structuring factors of meiofauna communities in a tropical seagrass bed (Gazi Bay, Kenya). Journal of Sea Research 45, 4561.
Doi, H., Chang, K.H. and Nakano, S. (2011) Nitrogen and carbon isotope fractionations of zooplankton consumers in ponds: potential effects of seston C:N stoichiometry. Marine and Freshwater Research 62, 6671.
Doi, H., Matsumasa, M., Fujikawa, M., Kanou, K., Suzuki, T. and Kikuchi, E. (2009) Macroalgae and seagrass contribution to gastropods in sub-tropical and temperate tidal flats. Journal of the Marine Biological Association of the United Kingdom 89, 399404.
Dubois, S., Jean-Louis, B., Bertrand, B. and Lefebvre, S. (2007) Isotope trophic-step fractionation of suspension-feeding species: implications for food partitioning in coastal ecosystems. Journal of Experimental Marine Biology and Ecology 351, 121128.
Ellison, A.M. (2004) Bayesian inference in ecology. Ecology Letters 7, 509520.
Escavarage, V., Gareía, M.E. and Castel, J. (1989) The distribution of meiofauna and its contribution to detritic pathways in tidal flats (Arcachon Bay, France). Scientia Marina 53, 551559.
Fry, B. (2006) Stable isotope ecology. New York: Springer.
Fry, B. and Sherr, E.B. (1984) δ13C measurements as indicators of carbon flow in marine and freshwater ecosystems. Contributions in Marine Science 27, 1347.
Heck, K., Able, K., Roman, C. and Fahay, M. (1995) Composition, abundance, biomass, and production of macro fauna in a New England estuary: comparisons among eelgrass meadows and other nursery habitats. Estuaries and Coasts 18, 379389.
Herman, P.M.J., Middelburg, J.J., Widdows, J., Lucas, C.H. and Heip, C.H.R. (2000) Stable isotopes as trophic tracers: combining field sampling and manipulative labelling of food resources for macrobenthos. Marine Ecology Progress Series 204, 7992.
Hilborn, R. and Mangel, M. (1997) The ecological detective. Princeton, NJ: Princeton University Press, 315 pp.
Holmer, M., Duarte, C.M., Boschker, H.T.S. and Barrón, C. (2004) Carbon cycling and bacterial carbon sources in pristine and impacted Mediterranean seagrass sediments. Marine Ecology Progress Series 36, 227237.
Incze, L.S., Mayer, L.M., Sherr, E.B. and Makco, S.A. (1982) Carbon inputs to bivalve mollusks: a comparison of two estuaries. Canadian Journal of Fisheries and Aquatic Sciences 39, 13481352.
Jaschinski, S., Brepohl, D.C. and Sommer, U. (2008) Carbon sources and trophic structure in an eelgrass Zostera marina bed, based on stable isotope and fatty acid analyses. Marine Ecology Progress Series 358, 103114.
Kanaya, G., Takagi, S. and Kikuchi, E. (2008) Spatial dietary variations in Laternula marilina (Bivalvia) and Hediste spp. (Polychaeta) along environmental gradients in two brackish lagoons. Marine Ecology Progress Series 359, 133144.
Kang, C.H., Kim, J.B., Lee, K.S., Kim, J.B., Lee, P.Y. and Hong, J.S. (2003) Trophic importance of benthic microalgae to macrozoobenthos in coastal bay systems in Korea: dual stable C and N isotope analyses. Marine Ecology Progress Series 259, 7992.
Kang, C.K., Choy, E.J., Paik, S.K., Park, H.J., Lee, K.S. and An, S. (2007) Contributions of primary organic matter sources to macroinvertebrate production in an intertidal salt marsh (Scirpus triqueter) ecosystem. Marine Ecology Progress Series 334, 131143.
Kenworthy, W.J. and Thayer, G.W. (1984) Production and decomposition of the roots and rhizomes of seagrasses Zostera marina and Thalassia testudinum, in temperate and subtropical marine ecosystems. Bulletin of Marine Science 35, 364379.
Lebreton, B., Richar, P., Galois, R., Radenac, G., Brahmia, A., Colli, G., Grouazel, M., André, C., Guillou, G. and Blanchard, G. (2012) Food sources used by sediment meiofauna in intertidal Zostera noltii seagrass bed: a seasonal stable isotope study. Marine Biology 159, 15371550.
Leduc, D. and Probert, P.K. (2009) The effect of bacterivorous nematodes on detritus incorporation by macrofaunal detritivores: a study using stable isotope and fatty acid analyses. Journal of Experimental Marine Biology and Ecology 371, 130139.
Lee, S.Y., Fong, C.W. and Wu, R.S.S. (2001) The effect of structure of seagrass (Zostera japonica) canopy structure on associated fauna: a study using artificial seagrass units and sampling of natural beds. Journal of Experimental Marine Biology and Ecology 259, 2350.
Lee, S.Y., Lee, S.M. and Choi, C.I. (2005) Phenology and reproductive effort of two Zostera marina L. populations on the southern coast of Korea. Ocean and Polar Research 27, 6774.
McCutchan, J.H., Lewis, W.M., Kendall, C. and McGrath, C.C. (2003) Variation in trophic shift for stable isotope ratios of carbon, nitogen, and sulfur. Oikos 102, 378390.
Minagawa, M. and Wada, E. (1984) Stepwise enrichment of 15N along food chains: further evidence and the relation between δ15N and animal age. Geochimica et Cosmochimica Acta 48, 11351140.
Montagna, P.A. (1984) In situ measurement of meiobenthic grazing rates on sediment bacteria and edaphic diatoms. Marine Ecology Progress Series 18, 119130.
Moore, J.W. and Semmens, B.X. (2008) Incorporating uncertainty and prior information into stable isotope mixing models. Ecology Letters 11, 470480.
Olsen, Y., Fox, S.E., Teichberg, M., Otter, M. and Valiela, I. (2011) δ15N and δ13C reveal differences in carbon flow through estuarine benthic food webs in response to the relative availability of macroalgae and eelgrass. Marine Ecology Progress Series 421, 8396.
Ouisse, V., Riera, P., Migné, A., Leroux, C. and Davoult, D. (2012) Food web analysis in intertidal Zostera marina and Zostera noltii communities in winter and summer. Marine Biology 159, 165175.
Page, H.M. (1997) Importance of vascular plant and algal production to macro-invertebrate consumers in a southern California salt marsh. Estuarine, Coastal and Shelf Science 45, 823834.
Peterson, B.J. and Fry, B. (1987) Stable isotopes in ecosystem studies. Annual Review of Ecology, Evolution, and Systematics 18, 293320.
Riera, P. and Richard, P. (1996) Isotopic determination of food sources of Crassostrea gigas along a trophic gradient in the estuarine bay of Marennes-Oleron. Estuarine, Coastal and Shelf Science 42, 347360.
Riera, P., Stal, L. and Nieuwenhuize, J. (2000) Heavy δ15N in intertidal benthic algae and invertebrates in the Scheldt Estuary (The Netherlands): effect of riverine nitrogen inputs. Estuarine, Coastal and Shelf Science 51, 365372.
Schaal, G., Riera, P. and Leroux, C. (2008) Trophic coupling between two adjacent benthic food webs within a man-made intertidal area: a stable isotopes evidence. Estuarine, Coastal and Shelf Science 77, 523534.
Shaffer, G.P. and Sullivan, M.J. (1988) Water column productivity attributable to displaced benthic diatoms in well-mixed shallow estuaries. Journal of Phycology 24, 132140.
Sinenstad, C.A. and Wissmar, R.C. (1985) δ13C evidence of origins and fates of organic carbon in estuarine and nearshore food webs. Marine Ecology Progress Series 22, 141152.
Tenore, K.R., Cammen, L., Findlay, S.E.G. and Phillips, N. (1982) Perspectives of research on detritus: do factors controlling the availability of detritus to macroconsumers depend on its source? Journal of Marine Research 40, 473489.
Tenore, K.R., Tietjen, J.H. and Lee, J.J. (1977) Effect of meiofauna on incorporation of aged eelgrass, Zostera marina, detritus by polychaete Nephthys incisa. Journal of the Fisheries Research Board of Canada 34, 563567.
Valentine, J.F. and Duffy, J.E. (2006) The central role of grazing in seagrass ecology. In Larkum, A.W.D., Orth, R.J. and Duarte, C.M. (eds) Seagrasses: biology, ecology and conservation. Dordrecht: Springer, pp. 463501.
Vanderklift, M.A. and Ponsard, S. (2003) Sources of variation in consumer-diet delta N-15 enrichment: a meta-analysis. Oecologia 136, 169182.
Vander Zanden, M.J. and Rasmussen, J.B. (2001) Variation in delta N-15 and delta C-13 trophic fractionation: implications for aquatic food web studies. Limnology and Oceanography 46, 20612066.
Warwick, R.M. (1987) Meiofauna: their role in marine detrital systems. In Moriarty, D.J.W. and Pullin, R.S.V. (eds) Detritus and microbial ecology in aquaculture. ICLARM conference proceedings, Bellagio, Como, Italy, 26–31 August 1985. Manila, Phillipines: International Center for Living Aquatic Resources Management, pp. 282295.
Widdows, J., Brinsley, M.D., Bowley, N. and Barrett, C. (1998) A benthic annular flume for in situ measurement of suspension feeding/biodeposition rates and erosion potential of intertidal cohesive sediments. Estuarine, Coastal and Shelf Science 46, 238.
Williams, C.J., Jaffé, R., Anderson, W.T. and Jochem, F.J. (2009) Importance of seagrass as a carbon source for heterotrophic bacteria in a subtropical estuary (Florida Bay). Estuarine, Coastal and Shelf Science 85, 507514.
Zurburg, W., Smaal, A.A.D., Héral, M. and Danker, N. (1994) Seston dynamics and bivalve feeding in the Bay of Marennes-Oléron (France). Netherlands Journal of Aquatic Ecolgy 28, 459466.


Trophic importance of meiofauna to polychaetes in a seagrass (Zostera marina) bed as traced by stable isotopes

  • Sun_Yong Ha (a1), Won-Ki Min (a2), Dong-Sung Kim (a2) and Kyung-Hoon Shin (a1)


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