Hostname: page-component-76fb5796d-dfsvx Total loading time: 0 Render date: 2024-04-25T16:24:04.097Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on benzo[a]pyrene mono-oxygenase in the shore crab Carcinus maenas

Published online by Cambridge University Press:  11 May 2009

S. C. M. O'Hara ,
E. D. S. Corner ,
T. E. V. Forsberg  and
M. N. Moore
S. C. M. O'Hara
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
E. D. S. Corner
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
T. E. V. Forsberg
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
M. N. Moore
Affiliation:
Natural Environment Research Council, Institute for Marine Environmental Research, Prospect Place, The Hoe, Plymouth, PL1 3DH
Get access Rights & Permissions [Opens in a new window]

Extract

A cytochrome P-450-dependent benzo[a]pyrene mono-oxygenase enzyme system (BPM) has been identified and partially characterized in males of the shore crab Carcinus maenas (L.). Apparent Km values obtained at 30 °C using microsomal preparations from the antennary glands of animals collected during summer were in the range 1.61–2.11 µM. The cytochrome P-450 content was 0·022 nmol/mg microsomal protein when BPM activity in the same preparation was 0·085 nmol/mg protein/min.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 62 , Issue 2 , May 1982 , pp. 339 - 357
Copyright
Copyright © Marine Biological Association of the United Kingdom 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ahsanullah, M. & Newell, R. C., 1971. Factors affecting the heart rate of the shore crab Carcinus tnaenas (L.). Comparative Biochemistry and Physiology, 39 A, 277–287.Google Scholar
Altman, F. P., 1972. Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases. Progress in Histochemistry and Cytochemistry, 4, 225–273.Google Scholar
Altman, F. P., 1976. Tetrazolium salts and formazans. Progress in Histochemistry and Cytochemistry, 9, 1–56.Google Scholar
Bayne, B. L., Moore, M. N., Widdows, J., Livingstone, D. R. & Salkeld, P., 1979. Measurement of the responses of individuals to environmental stress and pollution. Philosophical Transactions of the Royal Society (B), 286, 563–581.Google Scholar
Bend, J. R., James, M. O. & Dansette, P. M., 1977. In vitro metabolism of xenobiotics in some marine animals. Annals of the New York Academy of Sciences, 298, 505–521.CrossRefGoogle Scholar
Burns, K. A., 1976. Hydrocarbon metabolism in the intertidal fiddler crab Uca pugnax. Marine Biology, 36, 5–11.Google Scholar
Charniaux-Cotton, H., 1960. Sex determination. In The Physiology of Crustacea, vol. 1. Metabolism and Growth (ed. Waterman, T. H.), pp. 411–447. Academic Press.Google Scholar
Chayen, J., 1978. Microdensitometry. In Biochemical Mechanisms of Liver Injury (ed. Slater, T. F.), pp. 259–292. Academic Press.Google Scholar
Conney, A. H., 1967. Pharmacological implications of microsomal enzyme induction. Pharmacological Reviews, 19, 317–366.Google Scholar
Dewaide, J. H., 1970. Species differences in hepatic drug oxidation in mammals and fishes in relation to thermal acclimation. Comparative General Pharmacology, 1, 375–384.CrossRefGoogle ScholarPubMed
Estabrook, R. W. & Werringloer, J., 1978. The measurement of difference spectra: application to the cytochromes of microsomes. In Methods ofEnzymology, vol. LII. Biomembranes: Part C: Biological Oxidations, Microsomal, Cytochrome P-450, and other Hemoprotein Systems (ed. Fleischer, S. and Packer, L.), pp. 212–220. Academic Press.Google Scholar
Harptree, E. F., 1972. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry, 48, 422–427.CrossRefGoogle Scholar
James, M. O., Khan, M. A. Q. & Bend, J. R., 1979. Hepatic microsomal mixed-function oxidase activities in several marine species common to coastal Florida. Comparative Biochemistry and Physiology, 63 C, 155–164.Google Scholar
Koudstaal, J. & Hardonk, M. J., 1969. Histochemical demonstration of enzymes related to NADPH-dependent hydroxylating systems in rat liver after phenobarbital treatment. Histochemie, 23, 68–77.CrossRefGoogle Scholar
Koudstaal, J. & Hardonk, M. J., 1972. Relations between biochemically determined hydroxylations and some enzyme-histochemical reactions in rat liver after phenobarbital and methylcholanthrene treatment. Acta histochemica, supplement, XII, 279–282.Google Scholar
Lee, R. F., 1981. Alixed function oxygenases (MFO) in marine invertebrates. Marine Biology Letters, 2, 87–105.Google Scholar
Lee, R. F., Furlong, E. & Singer, S. C., 1977. Metabolism of hydrocarbons in marine invertebrates: aryl hydrocarbon hydroxylase from the tissues of the blue crab, Callinectes sapidus and the polychaete worm Nereis sp. In Pollutant Effects on Marine Organisms (ed. Giam, C. S.), pp. 111–124. Lexington, D.C.: Heath and Company.Google Scholar
Lee, R. F., Singer, S. C. & Page, D. S., 1982. Responses of cytochrome P-450 systems in marine crabs and polychaetes to organic pollutants. Aquatic Toxicology, 1, 355–365.CrossRefGoogle Scholar
Low, P. S., Bada, J. L. & Somero, G. N., 1973. Temperature adaptation of enzymes: roles of the free energy, the enthalpy, and the entropy of activation. Proceedings of the National Academy of Sciences of the United States of America, 70, 430–432.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, N. J., 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.Google Scholar
Maynard, D. M., 1960. Circulation and heart function. In The Physiology of Crustacea, vol. I. Metabolism and Growth (ed. Waterman, T. H.), pp. 161–226. Academic Press.Google Scholar
Moore, M. N., 1979. Cellular responses to polycyclic aromatic hydrocarbons and phenobarbital in Mytilus edulis. Marine Environmental Research, 2, 255–263.Google Scholar
Moore, M. N., 1980. Cytochemical determination of cellular responses to environmental stressors in marine organisms. Rapport et procè-verbaux des réunions. Conseil permanent international pour l' exploration de la mer, 179, 7–15.Google Scholar
Naylor, E., 1958. Tidal and diurnal rhythms of locomotory activity in Carcinus maenas (L.). Journal of Experimental Biology, 35, 481–488.Google Scholar
Naylor, E. & Williams, B. G., 1968. Effects of eyestalk removal on rhythmic locomotor activity in Carcinus. Journal of Experimental Biology, 49, 107–116.Google Scholar
Nebert, D. W. & Gelboin, H. V., 1968. Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. Journal of Biological Chemistry, 243, 6242–6249.CrossRefGoogle ScholarPubMed
Nebert, D. W. & Gielen, J. E., 1972. Genetic regulation of aryl hydrocarbon hydroxylase induction in the mouse. Federation Proceedings, 31, 1315–1325.Google ScholarPubMed
O'Hara, S. C. M., Gaskell, S. J. & Corner, E. D. S., 1979. On the nutrition and metabolism of zooplankton. XIII. Further studies of steroids in Calanus. Journal of the Marine Biological Association of the United Kingdom, 59, 331–340.Google Scholar
Palmork, K. H. & Solbakken, J. E., 1979. Accumulation and metabolism of phenanthrene in Norway lobster (Nephrops norvegicus). International Council for the Exploration of the Sea (C.M. Papers and Reports), E: 53, 8 pp.Google Scholar
Payne, J. F., 1976. Field evaluation of benzopyrene hydroxylase induction as a monitor for marine petroleum pollution. Science, New York, 191, 945–946.Google Scholar
Payne, J. F., 1977. Mixed function oxidases in marine organisms in relation to petroleum hydrocarbon metabolism and detection. Marine Pollution Bulletin, 8, 112–116.CrossRefGoogle Scholar
Rajan, K. D., Kharouf, H. H. & Lockwood, A. P. M., 1979. Rhythmic cycles of blood sugar concentrations in the crab Carcinus maenas. In Cyclic Phenomena in Marine Plants and Animals. Proceedings of the 13th European Marine Biology Symposium, Isle of Man, 1978 (ed. Naylor, E. and Hartnoll, R. G.), pp. 451–458. Pergamon Press.Google Scholar
Singer, S. C. & Lee, R. F., 1977. Mixed function oxygenase activity in the crab, Callinectes sapidus: tissue distribution and correlation with changes during molting and development. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 153, 377–386.CrossRefGoogle Scholar
Stegeman, J. J., 1978. Influence of environmental contamination on cytochrome P-450 mixedfunction oxygenases in fish: implications for recovery in the Wild Harbor Marsh. Journal of the Fisheries Research Board of Canada, 35, 668–674.Google Scholar
Stegeman, J. J., 1979. Temperature influence on basal activity and induction of mixed-function oxygenase activity in Fundulus heteroclitus. Journal of the Fisheries Research Board of Canada, 36, 1400–1405.Google Scholar
Stegeman, J. J., Binder, R. L. & Orren, A., 1979. Hepatic and extrahepatic microsomal electron transport components and mixed-function oxygenases in the marine fish Stenotomus versicolor. Biochemical Pharmacology, 28, 3431–3439.CrossRefGoogle ScholarPubMed
Stegeman, J. J. & Kaplan, H. B., 1981. Mixed-function oxygenase activity and benzo[a]pyrene metabolism in the barnacle Balanus eburneus (Crustacea, Cirripedia). Comparative Biochemistry and Physiology, 68 C, 55–61.Google Scholar
Teshima, S., 1972. Studies on the sterol metabolism in marine crustaceans. Memoirs of the Faculty of Fisheries, Kagoshima University, 21, 69–147.Google Scholar
Uemura, T. & Chiesara, C., 1976. NADH-dependent aryl hydrocarbon hydroxylase in rat liver mitochondrial outer membrane. European Journal of Biochemistry, 66, 293–307.Google Scholar
Varanasi, U. & Malins, D. C., 1977. Metabolism of petroleum hydrocarbons: accumulation and biotransformation in marine organisms. In Effects of Petroleum on Arctic and Subarctic Marine Environments and Organisms, vol. 2. Biological Effects (ed. Malins, D. C.), pp. 175–270. Academic Press.Google Scholar
Walters, J. M., Cain, R. B., Higgins, I. J. & Corner, E. D. S., 1979. Cell-free benzo[a]pyrene hydroxylase activity in marine zooplankton. Journal of the Marine Biological Association of the United Kingdom, 59, 553–563.Google Scholar
Williams, B. G. & Naylor, E., 1967. Spontaneously induced rhythm of tidal periodicity in laboratory-reared Carcinus. Journal of Experimental Biology, 47, 229–234.Google Scholar