Hostname: page-component-7bb8b95d7b-s9k8s Total loading time: 0 Render date: 2024-09-22T20:38:34.288Z Has data issue: false hasContentIssue false
  • English
  • Français

Structural variability and distribution of cells in a posterior gill of Carcinus maenas (Decapoda: Brachyura)

Published online by Cambridge University Press:  09 October 2019

S.L. Lawson ,
M.B. Jones  and
R.M. Moate
M.B. Jones
Affiliation:
Ecotoxicology and Marine Biology Research Group, Department of Biological Sciences and Electron Microscopy Centre, University of Plymouth, Drake Circus, Plymouth, PL4 8AA
R.M. Moate
Affiliation:
Ecotoxicology and Marine Biology Research Group, Department of Biological Sciences and Electron Microscopy Centre, University of Plymouth, Drake Circus, Plymouth, PL4 8AA
Get access Rights & Permissions [Opens in a new window]

Abstract

Gill number 8 of intermoult male Carcinus maenas (L.) (Decapoda: Brachyura) was used to study the morphological variation and distribution of the major gill cells. Three gill regions (proximal, mid and distal) were sectioned and examined by light and transmission electron microscopy. The ultrastructure of the three epithelial cell types (striated, chief and pillar) was similar to previously published descriptions, but some variations in cell form and distribution were observed. In all gill regions, striated and chief cells were larger towards the lamellar raphe than near the marginal canal. In the proximal gill region, the epithelium consisted almost exclusively of striated cells, implying an osmotic/ionic role for this region. In the mid gill region, the epithelium comprised a mixture of chief and striated cells. In the distal region, chief cells predominated, implying a respiratory role for this region. Pillar cells exhibited no clear distributional pattern within the gill and, although their shape varied with position, their involvement in ionic regulation, osmoregulation, respiration, structural support or the flow of haemolymph through the gill is not clearly defined. Nephrocytes, thought to be involved in gill defence mechanisms, occurred in the haemolymph space or attached to the intralamellar septum and showed no structural variability. Glycocytes were always associated with the intralamellar septum, implying that there are specific gill regions for glycogen storage. Haemocytes showed some structural variation but there was no obvious correlation with gill region; small-granule haemocytes were the most common haemocyte type in the gill.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 74 , Issue 4 , November 1994 , pp. 771 - 785
Copyright
Copyright © Marine Biological Association of the United Kingdom 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barra, J.-A., Pequeux, A. & Humbert, W., 1983. A morphological study on gills of a crab acclimated to fresh water. Tissue and Cell, 15, 583–596.Google Scholar
Berridge, M.J. & Oschman, J.L., 1972. Transporting epithelia. New York: Academic Press.Google Scholar
Bodammer, J.E., 1978. Cytological observations on the blood and hemopoietic tissue in the crab, Callinectes sapidus. I. The fine structure of the hemocytes from intermoult animals. Cell and Tissue Research, 187, 79–96.Google Scholar
Bubel, A., 1976. Histological and electron microscopical observations on the effects of different salinities and heavy metal ions, on the gills of Jaera nordmanni (Rathke) (Crustacea, Isopoda). Cell and Tissue Research, 167, 65–95.Google Scholar
Bubel, A. & Jones, M.B., 1974. Fine structure of the gills of Jaera nordmanni (Rathke) (Crustacea, Isopoda). Journal of the Marine Biological Association of the United Kingdom, 54, 737–743.Google Scholar
Compere, Ph., Wanson, S., Pequeux, A., Gilles, R. & Goffinet, G., 1989. Ultrastructural changes in the gill epithelium of the green crab Carcinus maenas in relation to the external salinity. Tissue and Cell, 21, 299–318.Google Scholar
Copeland, D.E., 1964. Salt absorbing cells in gills of crabs Callinectes and Carcinus . Biological Bulletin. Marine Biological Laboratory, Woods Hole, 127, 367–368.Google Scholar
Copeland, D.E., 1968. Fine structure of salt and water uptake in the land-crab, Gecarcinus lateralis . American Zoologist, 8, 417–432.Google Scholar
Copeland, D.E & Fitzjarrell, A.T., 1968. The salt absorbing cells in the gills of the blue crab (Callinectes sapidus Rathbun) with notes on modified mitochondria. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 92, 1–22.Google Scholar
Copeland, E., 1963. Possible osmoregulatory cells in crab gills. Journal of Cell Biology, 19, 16A.Google Scholar
Cornick, J.W. & Stewart, J.S., 1978. Lobster (Homarus americanus) hemocytes: classification, differential counts and associated agglutinin activity. Journal of Invertebrate Pathology, 31, 194–203.Google Scholar
Cuenot, L., 1893. Études physiologiques sur les Crustacés Decapodes. Archives de Biologie, 13, 245–303.Google Scholar
Doughtie, D.G. & Rao, K.R., 1981. The syncytial nature and phagocytic activity of the branchial podocytes in the grass shrimp, Palaemonetes pugio . Tissue and Cell, 13, 93–104.Google Scholar
Drach, P., 1930. Étude sur le système branchiale des Crustacés Decapodés. Archives d'Anatomie Microscopique, Paris, 26, 83–133.Google Scholar
Evans, P.D., 1972. The free amino acid pool of the haemocytes of Carcinus maenas (L.). Journal of Experimental Biology, 56, 501–507.Google Scholar
Farrelly, C.A. & Greenaway, P., 1992. Morphology and ultrastructure of the gills of terrestrial crabs (Crustacea, Gecarcinidae and Grapsidae) – adaptations to air breathing. Zoomorphology, 112, 39–49.Google Scholar
Finol, H.J. & Croghan, P.C., 1983. Ultrastructure of the branchial epithelium of an amphibious brackish-water crab. Tissue and Cell, 15, 63–75.Google Scholar
Fisher, J.M., 1972. Fine-structural observations on the gill filaments of the freshwater crayfish, Astacus pallipes Lereboullet. Tissue and Cell, 4, 287–299.Google Scholar
Fontaine, C.T. & Lightner, D.V., 1973a. Observations on the process of wound repair in penaeid shrimp. Journal of Invertebrate Pathology, 22, 23–33.Google Scholar
Fontaine, C.T. & Lightner, D.V., 1973b. Observations on the phagocytosis and elimination of carmine particles injected into the abdominal musculature of the white shrimp, Penaeus setiferus . Journal of Invertebrate Pathology, 24, 141–148.Google Scholar
Fontaine, C.T. & Lightner, D.V., 1975. Cellular response to injury in penaeid shrimp. Marine Fisheries Research. Marine Laboratory, University of Miami, 37(5–6), 4–10.Google Scholar
Foster, C.A. & Howse, H.D., 1978. A morphological study on gills of the brown shrimp, Penaeus aztecus . Tissue and Cell, 10, 77–92.Google Scholar
Ghiretti-Magaldi, A., Milanesi, C. & Tognon, G., 1977. Hemopoiesis in Crustacea Decapoda: origin and evolution of hemocytes and cyanocytes of Carcinus maenas . Cell Differentiation, 6, 167–186.Google Scholar
Gilles, R. & Pequeux, A., 1981. Cell volume regulation in crustaceans: relationship between mechanisms for controlling the osmolarity of extracellular and intracellular fluids. Journal of Experimental Zoology, 215, 351–362.Google Scholar
Goodman, S.H. & Cavey, M.J., 1990. Organization of a phyllobranchiate gill from the green shore crab Carcinus maenas (Crustacea, Decapoda). Cell and Tissue Research, 260, 495–505.Google Scholar
Green, C.R. & Bergquist, P.R., 1982. Phylogenetic relationships within the Invertebrata in relation to the structure of septate junctions and the development of ‘occluding’ junctional types. Journal of Cell Science, 53, 279–305.Google Scholar
Hearing, V. & Vernick, S.H., 1967. Fine structure of the blood cells of the lobster, Homarus americanus . Chesapeake Science, 8, 170–186.Google Scholar
Johnson, P.T., 1980. Histology of the blue crab Callinectes sapidus. A model for the Decapoda. New York: Praeger Publishers.Google Scholar
Johnston, M.A. & Davies, P.S., 1972. Carbohydrates of the hepatopancreas and blood tissues of Carcinus . Comparative Biochemistry and Physiology, 41B, 433–443.Google Scholar
Johnston, M.A., Elder, H.Y. & Davies, P.S., 1973. Cytology of Carcinus haemocytes and their function in carbohydrate metabolism. Comparative Biochemistry and Physiology, 46A, 569–581.Google Scholar
Lochhead, J.H. & Lochhead, M.S., 1941. Studies on the blood and related tissues in Artemia (Crustacea, Anostraca). Journal of Morphology, 68, 593–632.Google Scholar
Loewenstein, W.R. & Kanno, Y., 1964. Studies on an epithelial (gland) cell junction. 1. Modifications of surface membrane permeability. Journal of Cell Biology, 22, 565–586.Google Scholar
Maina, J.N., 1990. The morphology of the gills of the freshwater African crab Potamon niloticus (Crustacea: Brachyura: Potamonidae): a scanning and transmission electron microscopic study. Journal of Zoology, 221, 499–515.Google Scholar
Martelo, M.-J. & Zanders, I.P., 1986. Modifications of gill ultrastructure and ionic composition in the crab Goniopsis cruentata acclimated to various salinities. Comparative Biochemistry and Physiology, 84A, 383–389.Google Scholar
Martin, G.G. & Graves, B.L., 1985. Fine structure and classification of shrimp hemocytes. Journal of Morphology, 185, 339–348.Google Scholar
Morse, H.C., Harris, P.J. & Dornfield, E.J., 1970. Pacifasticus leniusculus: fine structure of arthrobranch with reference to active ion uptake. Transactions of the American Microscopical Society, 89, 12–27.Google Scholar
Noiret-Timothee, C. & Noiret, C., 1980. Septate and sclariform junctions in arthropods. International Review of Cytology, 63, 97–140.Google Scholar
Paterson, W.D. & Stewart, J.E., 1974. In vitro phagocytosis by hemocytes of the American lobster (Homarus americanus). Journal of the Fisheries Research Board of Canada, 31, 1051–1056.Google Scholar
Pequeux, A. & Gilles, R., 1981. Na+ fluxes across isolated perfused gills of the Chinese crab Eriocheir sinensis . Journal of Experimental Biology, 92, 173–186.Google Scholar
Rabin, H., 1970. Hemocytes, hemolymph and defence reactions in crustaceans. Research Journal of the Reticuloendothelial Society, 7, 195–207.Google Scholar
Ratcliffe, N.A. & Rowley, A.F., 1979. A comparative synopsis of the structure and function of the blood cells of insects and other invertebrates. Developments in Comparative Immunology, 3, 189–243.Google Scholar
Sawyer, T.K., Cox, R. & Higginbottom, M., 1970. Hemocyte values in healthy blue crabs, Callinectes sapidus, and crabs infected with the amoeba, Paramoeba perniciosa . Journal of Invertebrate Pathology, 15, 440–446.Google Scholar
Sewell, M.T., 1955. Lipo-protein cells in the blood of Carcinus maenas, and their cycle of activity correlated with the moult. Quarterly Journal of Microscopical Science, 96, 73–83.Google Scholar
Sindermann, C.J., 1971. Internal defences of Crustacea: a review. Fishery Bulletin. National Oceanic and Atmospheric Administration. Washington, DC, 69, 455–489.Google Scholar
Smith, V.J. & Ratcliffe, N.A., 1978. Host defence reactions of the shore crab, Carcinus maenas (L.), in vitro . Journal of the Marine Biological Association of the United Kingdom, 58, 357–379.Google Scholar
Strangeways-Dixon, J. & Smith, D.S., 1970. The fine structure of gill ‘podocytes’ in Palinurus argus (Crustacea). Tissue and Cell, 2, 611–624.Google Scholar
Stutman, L.J. & Dolliver, M., 1968. Mechanism Of Coagulation In Gecarcinus Lateralis . American Zoologist, 8, 481–489.Google Scholar
Talbot, P., Clark, W.H. Jr & Lawrence, A.L., 1972. Light and electron microscopic study on osmoregulatory tissue in the developing brown shrimp, Penaeus aztecus . Tissue and Cell, 4, 271–286.Google Scholar
Taylor, H.H. & Greenaway, P., 1979. The structure of the gills and lungs of the arid-zone crab Holthuisana (Austrothelphusa) transversa (Brachyura: Sundathelphusidae) including observations on arterial vessels within the gills. Journal of Zoology, 189, 359–384.Google Scholar
Taylor, H.H. & Taylor, E.W., 1986. Observations of valve-like structures and evidence for rectification of flow within the gill lamellae of the crab Carcinus maenas (Crustacea, Decapoda). Zoomorphology, 106, 1–11.Google Scholar
Taylor, H.H. & Taylor, E.W., 1992. Gills and lungs: the exchange of gases and ions. Microscopical Anatomy of Invertebrates, 10, 203–293.Google Scholar
Toney, M.E. Jr, 1958. Morphology of the blood cells of some Crustacea. Growth, 22, 35–50.Google Scholar
Towle, D.W. & Kays, W.T., 1986. Basolateral localization of Na+ K+-ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas . Journal of Experimental Zoology, 239, 311–318.Google Scholar
Williams, A.J. & Lutz, P.L., 1975. Blood cell types in Carcinus maenas and their physiological roles. Journal of the Marine Biological Association of the United Kingdom, 55, 671–674.Google Scholar
Wood, P.J., Podlewski, J. & Shenk, T.E., 1971. Cytochemical observations of hemolymph cells during coagulation in the crayfish, Orconectes virilis . Journal of Morphology, 134, 479–488.Google Scholar
Wright, K.A., 1964. The fine structure of the nephrocyte of the gills of two marine decapods. Journal of Ultrastructural Research, 10, 1–13.Google Scholar
Zanders, I.P., 1981. Control and dynamics of ionic balance in Carcinus maenas (L.). Comparative Biochemistry and Physiology, 70A, 457–468.Google Scholar