Skip to main content Accessibility help
×
Home

Structural variability and distribution of cells in a posterior gill of Carcinus maenas (Decapoda: Brachyura)

  • S.L. Lawson, M.B. Jones (a1) and R.M. Moate (a1)

Abstract

Gill number 8 of intermoult male Carcinus maenas (L.) (Decapoda: Brachyura) was used to study the morphological variation and distribution of the major gill cells. Three gill regions (proximal, mid and distal) were sectioned and examined by light and transmission electron microscopy. The ultrastructure of the three epithelial cell types (striated, chief and pillar) was similar to previously published descriptions, but some variations in cell form and distribution were observed. In all gill regions, striated and chief cells were larger towards the lamellar raphe than near the marginal canal. In the proximal gill region, the epithelium consisted almost exclusively of striated cells, implying an osmotic/ionic role for this region. In the mid gill region, the epithelium comprised a mixture of chief and striated cells. In the distal region, chief cells predominated, implying a respiratory role for this region. Pillar cells exhibited no clear distributional pattern within the gill and, although their shape varied with position, their involvement in ionic regulation, osmoregulation, respiration, structural support or the flow of haemolymph through the gill is not clearly defined. Nephrocytes, thought to be involved in gill defence mechanisms, occurred in the haemolymph space or attached to the intralamellar septum and showed no structural variability. Glycocytes were always associated with the intralamellar septum, implying that there are specific gill regions for glycogen storage. Haemocytes showed some structural variation but there was no obvious correlation with gill region; small-granule haemocytes were the most common haemocyte type in the gill.

Copyright

References

Hide All
Barra, J.-A., Pequeux, A. & Humbert, W., 1983. A morphological study on gills of a crab acclimated to fresh water. Tissue and Cell, 15, 583596.
Berridge, M.J. & Oschman, J.L., 1972. Transporting epithelia. New York: Academic Press.
Bodammer, J.E., 1978. Cytological observations on the blood and hemopoietic tissue in the crab, Callinectes sapidus. I. The fine structure of the hemocytes from intermoult animals. Cell and Tissue Research, 187, 7996.
Bubel, A., 1976. Histological and electron microscopical observations on the effects of different salinities and heavy metal ions, on the gills of Jaera nordmanni (Rathke) (Crustacea, Isopoda). Cell and Tissue Research, 167, 6595.
Bubel, A. & Jones, M.B., 1974. Fine structure of the gills of Jaera nordmanni (Rathke) (Crustacea, Isopoda). Journal of the Marine Biological Association of the United Kingdom, 54, 737743.
Compere, Ph., Wanson, S., Pequeux, A., Gilles, R. & Goffinet, G., 1989. Ultrastructural changes in the gill epithelium of the green crab Carcinus maenas in relation to the external salinity. Tissue and Cell, 21, 299318.
Copeland, D.E., 1964. Salt absorbing cells in gills of crabs Callinectes and Carcinus . Biological Bulletin. Marine Biological Laboratory, Woods Hole, 127, 367368.
Copeland, D.E., 1968. Fine structure of salt and water uptake in the land-crab, Gecarcinus lateralis . American Zoologist, 8, 417432.
Copeland, D.E & Fitzjarrell, A.T., 1968. The salt absorbing cells in the gills of the blue crab (Callinectes sapidus Rathbun) with notes on modified mitochondria. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 92, 122.
Copeland, E., 1963. Possible osmoregulatory cells in crab gills. Journal of Cell Biology, 19, 16A.
Cornick, J.W. & Stewart, J.S., 1978. Lobster (Homarus americanus) hemocytes: classification, differential counts and associated agglutinin activity. Journal of Invertebrate Pathology, 31, 194203.
Cuenot, L., 1893. Études physiologiques sur les Crustacés Decapodes. Archives de Biologie, 13, 245303.
Doughtie, D.G. & Rao, K.R., 1981. The syncytial nature and phagocytic activity of the branchial podocytes in the grass shrimp, Palaemonetes pugio . Tissue and Cell, 13, 93104.
Drach, P., 1930. Étude sur le système branchiale des Crustacés Decapodés. Archives d'Anatomie Microscopique, Paris, 26, 83133.
Evans, P.D., 1972. The free amino acid pool of the haemocytes of Carcinus maenas (L.). Journal of Experimental Biology, 56, 501507.
Farrelly, C.A. & Greenaway, P., 1992. Morphology and ultrastructure of the gills of terrestrial crabs (Crustacea, Gecarcinidae and Grapsidae) – adaptations to air breathing. Zoomorphology, 112, 3949.
Finol, H.J. & Croghan, P.C., 1983. Ultrastructure of the branchial epithelium of an amphibious brackish-water crab. Tissue and Cell, 15, 6375.
Fisher, J.M., 1972. Fine-structural observations on the gill filaments of the freshwater crayfish, Astacus pallipes Lereboullet. Tissue and Cell, 4, 287299.
Fontaine, C.T. & Lightner, D.V., 1973a. Observations on the process of wound repair in penaeid shrimp. Journal of Invertebrate Pathology, 22, 2333.
Fontaine, C.T. & Lightner, D.V., 1973b. Observations on the phagocytosis and elimination of carmine particles injected into the abdominal musculature of the white shrimp, Penaeus setiferus . Journal of Invertebrate Pathology, 24, 141148.
Fontaine, C.T. & Lightner, D.V., 1975. Cellular response to injury in penaeid shrimp. Marine Fisheries Research. Marine Laboratory, University of Miami, 37(5–6), 410.
Foster, C.A. & Howse, H.D., 1978. A morphological study on gills of the brown shrimp, Penaeus aztecus . Tissue and Cell, 10, 7792.
Ghiretti-Magaldi, A., Milanesi, C. & Tognon, G., 1977. Hemopoiesis in Crustacea Decapoda: origin and evolution of hemocytes and cyanocytes of Carcinus maenas . Cell Differentiation, 6, 167186.
Gilles, R. & Pequeux, A., 1981. Cell volume regulation in crustaceans: relationship between mechanisms for controlling the osmolarity of extracellular and intracellular fluids. Journal of Experimental Zoology, 215, 351362.
Goodman, S.H. & Cavey, M.J., 1990. Organization of a phyllobranchiate gill from the green shore crab Carcinus maenas (Crustacea, Decapoda). Cell and Tissue Research, 260, 495505.
Green, C.R. & Bergquist, P.R., 1982. Phylogenetic relationships within the Invertebrata in relation to the structure of septate junctions and the development of ‘occluding’ junctional types. Journal of Cell Science, 53, 279305.
Hearing, V. & Vernick, S.H., 1967. Fine structure of the blood cells of the lobster, Homarus americanus . Chesapeake Science, 8, 170186.
Johnson, P.T., 1980. Histology of the blue crab Callinectes sapidus. A model for the Decapoda. New York: Praeger Publishers.
Johnston, M.A. & Davies, P.S., 1972. Carbohydrates of the hepatopancreas and blood tissues of Carcinus . Comparative Biochemistry and Physiology, 41B, 433443.
Johnston, M.A., Elder, H.Y. & Davies, P.S., 1973. Cytology of Carcinus haemocytes and their function in carbohydrate metabolism. Comparative Biochemistry and Physiology, 46A, 569581.
Lochhead, J.H. & Lochhead, M.S., 1941. Studies on the blood and related tissues in Artemia (Crustacea, Anostraca). Journal of Morphology, 68, 593632.
Loewenstein, W.R. & Kanno, Y., 1964. Studies on an epithelial (gland) cell junction. 1. Modifications of surface membrane permeability. Journal of Cell Biology, 22, 565586.
Maina, J.N., 1990. The morphology of the gills of the freshwater African crab Potamon niloticus (Crustacea: Brachyura: Potamonidae): a scanning and transmission electron microscopic study. Journal of Zoology, 221, 499515.
Martelo, M.-J. & Zanders, I.P., 1986. Modifications of gill ultrastructure and ionic composition in the crab Goniopsis cruentata acclimated to various salinities. Comparative Biochemistry and Physiology, 84A, 383389.
Martin, G.G. & Graves, B.L., 1985. Fine structure and classification of shrimp hemocytes. Journal of Morphology, 185, 339348.
Morse, H.C., Harris, P.J. & Dornfield, E.J., 1970. Pacifasticus leniusculus: fine structure of arthrobranch with reference to active ion uptake. Transactions of the American Microscopical Society, 89, 1227.
Noiret-Timothee, C. & Noiret, C., 1980. Septate and sclariform junctions in arthropods. International Review of Cytology, 63, 97140.
Paterson, W.D. & Stewart, J.E., 1974. In vitro phagocytosis by hemocytes of the American lobster (Homarus americanus). Journal of the Fisheries Research Board of Canada, 31, 10511056.
Pequeux, A. & Gilles, R., 1981. Na+ fluxes across isolated perfused gills of the Chinese crab Eriocheir sinensis . Journal of Experimental Biology, 92, 173186.
Rabin, H., 1970. Hemocytes, hemolymph and defence reactions in crustaceans. Research Journal of the Reticuloendothelial Society, 7, 195207.
Ratcliffe, N.A. & Rowley, A.F., 1979. A comparative synopsis of the structure and function of the blood cells of insects and other invertebrates. Developments in Comparative Immunology, 3, 189243.
Sawyer, T.K., Cox, R. & Higginbottom, M., 1970. Hemocyte values in healthy blue crabs, Callinectes sapidus, and crabs infected with the amoeba, Paramoeba perniciosa . Journal of Invertebrate Pathology, 15, 440446.
Sewell, M.T., 1955. Lipo-protein cells in the blood of Carcinus maenas, and their cycle of activity correlated with the moult. Quarterly Journal of Microscopical Science, 96, 7383.
Sindermann, C.J., 1971. Internal defences of Crustacea: a review. Fishery Bulletin. National Oceanic and Atmospheric Administration. Washington, DC, 69, 455489.
Smith, V.J. & Ratcliffe, N.A., 1978. Host defence reactions of the shore crab, Carcinus maenas (L.), in vitro . Journal of the Marine Biological Association of the United Kingdom, 58, 357379.
Strangeways-Dixon, J. & Smith, D.S., 1970. The fine structure of gill ‘podocytes’ in Palinurus argus (Crustacea). Tissue and Cell, 2, 611624.
Stutman, L.J. & Dolliver, M., 1968. Mechanism Of Coagulation In Gecarcinus Lateralis . American Zoologist, 8, 481489.
Talbot, P., Clark, W.H. Jr & Lawrence, A.L., 1972. Light and electron microscopic study on osmoregulatory tissue in the developing brown shrimp, Penaeus aztecus . Tissue and Cell, 4, 271286.
Taylor, H.H. & Greenaway, P., 1979. The structure of the gills and lungs of the arid-zone crab Holthuisana (Austrothelphusa) transversa (Brachyura: Sundathelphusidae) including observations on arterial vessels within the gills. Journal of Zoology, 189, 359384.
Taylor, H.H. & Taylor, E.W., 1986. Observations of valve-like structures and evidence for rectification of flow within the gill lamellae of the crab Carcinus maenas (Crustacea, Decapoda). Zoomorphology, 106, 111.
Taylor, H.H. & Taylor, E.W., 1992. Gills and lungs: the exchange of gases and ions. Microscopical Anatomy of Invertebrates, 10, 203293.
Toney, M.E. Jr, 1958. Morphology of the blood cells of some Crustacea. Growth, 22, 3550.
Towle, D.W. & Kays, W.T., 1986. Basolateral localization of Na+ K+-ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas . Journal of Experimental Zoology, 239, 311318.
Williams, A.J. & Lutz, P.L., 1975. Blood cell types in Carcinus maenas and their physiological roles. Journal of the Marine Biological Association of the United Kingdom, 55, 671674.
Wood, P.J., Podlewski, J. & Shenk, T.E., 1971. Cytochemical observations of hemolymph cells during coagulation in the crayfish, Orconectes virilis . Journal of Morphology, 134, 479488.
Wright, K.A., 1964. The fine structure of the nephrocyte of the gills of two marine decapods. Journal of Ultrastructural Research, 10, 113.
Zanders, I.P., 1981. Control and dynamics of ionic balance in Carcinus maenas (L.). Comparative Biochemistry and Physiology, 70A, 457468.

Structural variability and distribution of cells in a posterior gill of Carcinus maenas (Decapoda: Brachyura)

  • S.L. Lawson, M.B. Jones (a1) and R.M. Moate (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed