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Seasonal and starvation-induced changes on gonads and lipid reserves of the digestive gland of Nucella lapillus (Caenogastropoda)

  • Catarina Inês Gonçalves (a1) and Alexandre Lobo-da-cunha (a1)

Abstract

This study was conceived to evaluate seasonal changes in the lipid reserves of the digestive gland of the carnivorous intertidal caenogastropod Nucella lapillus, using a stereological approach. Volume density of lipid droplets in the digestive gland, digestive gland weight and volume were assessed in animals collected in March, June, September and December on the Portuguese coast. Gonad development was evaluated to detect any relationship between lipid content in the digestive gland and the reproductive cycle. The quantitative light microscopic analysis demonstrates that lipid droplets are a major component of the digestive gland. In males, the digestive gland and its lipid reserves were quite stable without significant variations throughout the year. In females, the percentage of digestive gland volume occupied by lipid droplets was higher in June and December, coinciding with the highest values of digestive gland volume. Due to the conjugation of these two factors, in June and December the total amount of lipids in the digestive gland was substantially increased in females. In both males and females a relationship between the development status of the gonad and the lipid reserves of the digestive gland was not evident. However, significant differences in the digestive gland lipid reserves were detected between males and females in June and December, pointing to a sex related effect on lipid reserves. To evaluate the use of lipid reserves as energy source in N. lapillus, the consumption of digestive gland lipids was followed during a starvation experiment.

Copyright

Corresponding author

Correspondence should be addressed to: A. Lobo-da-Cunha, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), Rua Jorge, Viterbo Ferreira 28, 4050-313 Porto, Portugal email: alcunha@icbas.up.pt

References

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Barber, B.J. and Blake, N.J. (1991) Reproductive physiology. In Shumway, S.E. (ed.) Scallops: biology, ecology and aquaculture. New York: Elsevier, pp. 377428
Crothers, J.H. (1985) Dog-whelks: an introduction to the biology of Nucella lapillus (L.). Field Studies 6, 291360.
Da Silva, R.S.M. and Zancan, D.M. (1994) Seasonal variations of the carbohydrate and lipid metabolism in a land pulmonate gastropod, Megalobulimus oblongus . Comparative Biochemistry and Physiology, Part A: Physiology 108A, 337341.
Dimitriadis, V.K. and Andrews, E.B. (2000) Ultrastructural and cytochemical study of the digestive gland cells of the marine prosobranch mollusc Nucella lapillus (L.) in relation to function. Malacologia 42, 103112.
Dimitriadis, V.K. and Hondros, D. (1992) Effect of starvation and hibernation on the fine structural morphology of digestive gland cells of the snail Helix lucorum . Malacologia 34, 6373.
Feare, C.J. (1970) The reproductive cycle of the dog whelk Nucella lapillus . Proceedings of the Malacological Society of London 39, 125137.
Følsvik, N., Berge, J.A., Brevik, E.M. and Walday, M. (1999) Quantification of organotin compounds and determination of imposex in populations of dogwhelks (Nucella lapillus) from Norway. Chemosphere 38, 681–391.
Franchini, A. and Ottaviani, E. (1993) Histochemical and ultrastructural study of the digestive gland of the freshwater snail Panorbarius corneus (L.) (Gastropoda, Pulmonata). Animal Biology 2, 191198.
Galante-Oliveira, S., Oliveira, I., Ferreira, N., Santos, J.A., Pacheco, M. and Barroso, C. (2011) Nucella lapillus L. imposex levels after legislation prohibiting TBT antifoulants: temporal trends from 2003 to 2008 along the Portuguese coast. Journal of Environmental Monitoring 13, 304312.
Ganter, P. and Jollès, G. (1970) Histochimie normal et pathologique. Paris: Gauthier-Villars.
Hughes, R.N. (1972) Annual production of two Nova Scotian populations of Nucella lapillus (L.). Oecologia 8, 356370.
Kress, A., Schmekel, L. and Nott, J.A. (1994) Ultrastructure of the digestive gland in the opisthobranch mollusk, Runcina . Veliger 37, 358373.
Largen, M.J. (1967) The influence of water temperature upon the life of the dog-whelk Thais lapillus (Gastropoda: Prosobranchia). Journal of Animal Ecology 36, 207214.
Le Pennec, G., Le Pennec, M. and Beninger, G. (2001) Seasonal digestive gland dynamics of the scallop Pecten maximus in the Bay of Brest (France). Journal of the Marine Biological Association of the United Kingdom 81, 663671.
Leung, K.M.Y. and Furness, R.W. (2001) Survival, growth, metallothionein and glycogen levels of Nucella lapillus (L.) exposed to sub-chronic cadmium stress: the influence of nutritional state and prey type. Marine Environmental Research 52, 173194.
Litaay, M. and De Silva, S. (2003) Spawning season, fecundity and proximate composition of the gonads of wild-caught blacklip abalone (Haliotis rubra) from Port Fairy waters, south-eastern Australia. Aquatic Living Resources 16, 353361.
Lobo-da-Cunha, A. (1999) Ultrastructural and cytochemical aspects of the basophilic cells in the hepatopancreas of Aplysia depilans (Mollusca, Opistobranchia). Tissue and Cell 31, 816.
Lobo-da-Cunha, A. (2000) The digestive cells of the hepatopancreas in Aplysia depilans (Mollusca, Opisthobranchia): ultrastructural and cytochemical study. Tissue and Cell 32, 4957.
Lobo-da-Cunha, A., Kádár, E. and Serrão Santos, R. (2006) Histochemical and ultrastructural characterization of mantle storage cells in the hydrothermal-vent bivalve Bathymodiolus azoricus . Marine Biology 150, 253260.
Morais, S., Boaventura, D., Narciso, L., , P. and Hawkins, S.J. (2003) Gonad development and fatty acid composition of Patella depressa Pennant (Gastropoda: Prosobranchia) populations with different patterns of spatial distribution, in exposed and sheltered sites. Journal of Experimental Marine Biology and Ecology 294, 6180.
Morton, B. (2009) Recovery from imposex by a population of the dogwhelk, Nucella lapillus (Gastropoda: Caenogastropoda), on the southeastern coast of England since May 2004: a 52-month study. Marine Pollution Bulletin 58, 15301538.
Pazos, A.J., Ruíz, C., García-Martin, O., Abad, M. and Sánchez, J.L. (1996) Seasonal variations of the lipid content and fatty acid composition of Crassostrea gigas cultured in El Grove, Galicia, N.W. Spain. Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology 114, 171179.
Pazos, A.J., Sánchez, J.L., Román, G., Pérez-Parallé, M.L. and Abad, M. (2003) Seasonal changes in lipid classes and fatty acid composition in the digestive gland of Pecten maximus . Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology, 134, 367380.
Plejdrup, J.K., Simonsen, V., Pertoldi, C., Schøyen, M. and Bayley, M. (2006) Genetic and morphological diversity in populations of Nucella lapillus (L.; neogastropoda) in response to tributyltin contamination. Ecotoxicology and Environmental Safety 64, 146154.
Robinson, W.E., Wehling, W.E., Morse, M.P. and McLeod, G.C. (1981) Seasonal changes in soft-body component indices and energy reserves in the Atlantic deep-sea scallop, Placopecten magellanicus . Fishery Bulletin, US 79, 449458.
Royet, J.P. (1991) Stereology: a method for analyzing images. Progress in Neurobiology 37, 433474.
Santos, M.M., Castro, L.F., Vieira, M.N., Micael, J., Morabito, R., Massanisso, P. and Reis-Henriques, M.A. (2005) New insights into the mechanism of imposex induction in the dogwhelk Nucella lapillus . Comparative Biochemistry and Physiology, Part C: Toxicology and Pharmacology 141, 101109.
Scherle, W. (1970) A simple method for volumetry of organs in quantitative stereology. Mikroskopie 26, 5760.
Thiery, J.P. (1967) Mise en evidence des polysaccharides sur coupes fines en microscopic electronique. Journal de Microscopie 6, 9871018.
Voltzow, J. (1994) Gastropoda: Prosobranchia. In Harrison, F.W. and Kohn, A.J. (eds) Microscopic anatomy of invertebrates. Volume 5. New York: Wiley-Liss, pp. 111252.

Keywords

Seasonal and starvation-induced changes on gonads and lipid reserves of the digestive gland of Nucella lapillus (Caenogastropoda)

  • Catarina Inês Gonçalves (a1) and Alexandre Lobo-da-cunha (a1)

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