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Phorbol Ester-Induced Metamorphosis in the Parasitic Barnacle, Loxothylacus Panopaei

Published online by Cambridge University Press:  11 May 2009

Henrik Glenner  and
Birger Brodin
Henrik Glenner
Affiliation:
Institute of Zoology, University of Copenhagen, 15 Universitetsparken, DK-2100 Copenhagen, Denmark.
Birger Brodin
Affiliation:
Biochemical Department, August Krogh Institute, University of Copenhagen, 13 Universitetsparken, DK-2100 Copenhagen, Denmark
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Extract

In order to induce metamorphosis in cyprids of the parasitic barnacle Loxothylacus panopaei (Gissler) (Cirripedia: Rhizocephala: Kentrogonida), we tested a number of compounds (ZR-512, 20-hydroxyecdysone, 3-isobutyl-l-methylxanthine and phorbol-12,13- dibutyrate) all known to cause metamorphosis in thoracican barnacles. Only one agent, phorbol-12,13-dibutyrate, had an effect and induced cypris larvae to complete metamorphosis without previous attachment. This suggests that diacylglycerol-activated protein kinase C plays a role in the metamorphosis of kentrogonid rhizocephalans.

Rhizocephalans are highly specialized crustaceans adapted for parasitic life on decapods. The juvenile parasite passes through an internal growth stage, where a root-like trophic organ invades the tissue and haemocoelic fluid of the host (for detailed information on rhizocephalan life history, see Haeg, 1992). As in free-living barnacles, a cyprid larva is the settling stage, and in thoracicans a juvenile barnacle is formed underneath the carapace of the cyprid larva. However, the rhizocephalans develop a so-called kentrogon, which accomplishes the injection of the primordial parasite (Glenner & Høeg, 1995). The degree of specialization towards a specific host means that the cypris larvae of rhizocephalans are extremely selective in their choice of substrata. They will not settle unless a specific host or part of a host, e.g. the gills, is offered. For this reason, the study of settlement and metamorphosis of these species has met with great difficulties.

Type
Short Communications
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 77 , Issue 1 , February 1997 , pp. 261 - 264
Copyright
Copyright © Marine Biological Association of the United Kingdom 1997

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References

Borst, D.W. & Laufer, H., 1990. Methyl farnesoate: a JH-like compound in crustaceans. In Recent advances in comparative arthropod morphology, physiology and development. Vol. 1. Morphogenetic hormones of arthropods – roles in histogenesis, organogenesis, and morphogenesis (ed. A.P., Gupta), pp. 35–60. New Brunswick, NJ: Rutgers University Press.Google Scholar
Burns, D.J. & Bell, R.M., 1991. Protein kinase C contains two phorbol ester binding domains. Journal of Biological Chemistry, 266(27), 18330–18338.CrossRefGoogle ScholarPubMed
Cheung, P.J., 1974. The effect of ecdysterone on cyprids of Balanus eburneus Gould. Journal of Experimental Marine Biology and Ecology, 15, 223–229.Google Scholar
Clare, A.S., Thomas, R.F. & Rittschof, D., 1995. Evidence for the involvement of cyclic AMP in the pheromonal modulation of barnacle settlement. Journal of Experimental Biology, 198, 655–664.Google Scholar
Exton, J.H., 1994. Phosphatidylcholine breakdown and signal transduction. Biochimica et Biophysica Acta, 1212, 26–42.Google Scholar
Glenner, H. & Høeg, J.T., 1995. A new motile, multicellular stage involved in host invasion by parasitic barnacles. Nature, London, 377, 157–160.Google Scholar
Gomez, E.D., Faulkner, D.J., Newman, W.A. & Ireland, C., 1973. Juvenile hormone mimics: effect on cirriped crustacean metamorphosis. Science, New York, 179, 813–814.Google Scholar
Høeg, J.T., 1987. Male cypris metamorphosis and a new male larval form, the trichogon, in the parasitic barnacle Sacculina carcini (Crustacea: Cirripedia: Rhizocephala). Philosophical Transactions of the Royal Society B, 317, 47–63.Google Scholar
Høeg, J.T., 1992. Rhizocephala. In Microscopic anatomy of invertebrates. Vol. 9. Crustacea (ed. F.W., Harrison and A.G., Humes), pp. 313–345. New York: Wiley-Liss Incorporated.Google Scholar
Jensen, P.G., Moyse, J., Høeg, J. & Al-Yahya, H., 1994. Comparative SEM studies of lattice organs: putative sensory structures on the carapace of larvae from Ascothoracida and Cirripedia (Crustacea Maxillopoda Thecostraca). Acta Zoologica, 75, 125–142.Google Scholar
Palli, S.R. et al., 1994. A nuclear juvenile hormone-binding protein from larvae of Manduca sexta: a putative receptor for the metamorphic action of juvenile hormone. Proceedings of the National Academy of Science of the United States of America, 91, 6191–6195.CrossRefGoogle ScholarPubMed
Riddiford, L.M., 1993. Hormone receptors and the regulation of insect metamorphosis. Receptor, 3, 203–209.Google ScholarPubMed
Rittschof, D., Maki, J., Mitchell, R. & Costlow, J.D., 1986. Ion and neuropharmacological studies of barnacle settlement. Netherlands Journal of Sea Research, 20, 269–275.CrossRefGoogle Scholar
Walker, G., Clare, A.S., Rittschof, D. & Mensching, D., 1992. Aspects of the life-cycle of Loxothylacus panopaei (Gissler), a sacculinid parasite of the mud crab Rhithropanopeus harrisii (Gould): a laboratory study. Journal of Experimental Marine Biology and Ecology, 157, 181–193.Google Scholar
Young, S., Parker, P.J., Ullrich, A. & Stabel, S., 1987. Down-regulation of protein kinase C is due to an increased rate or degradation. Biochemical Journal, 244, 775–779.CrossRefGoogle ScholarPubMed