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Impact of temperature and growth phases on lipid composition and fatty acid profile of a thermophilic Bacillariophyta strain related to the genus Halamphora from north-eastern Tunisia

Published online by Cambridge University Press:  15 June 2020

Nahla Bouzidi Open the ORCID record for Nahla Bouzidi [Opens in a new window] ,
Fatma Zili ,
Federico García-Maroto ,
Diego López Alonso  and
Hatem Ben Ouada
Nahla Bouzidi*
Affiliation:
Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), National Institute of Marine Sciences and Technology, BP 59, 5000Monastir, Tunisia
Fatma Zili
Affiliation:
Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), National Institute of Marine Sciences and Technology, BP 59, 5000Monastir, Tunisia
Federico García-Maroto
Affiliation:
Department of Chemistry and Physics, University of Almeria, 04120Almería, Spain
Diego López Alonso
Affiliation:
Department of Biology and Geology, University of Almería, 04120Almería, Spain
Hatem Ben Ouada
Affiliation:
Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), National Institute of Marine Sciences and Technology, BP 59, 5000Monastir, Tunisia
*
Author for correspondence: Nahla Bouzidi, E-mail: bouzidi.nehla@gmail.com
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Abstract

A thermo-tolerant diatom species has been isolated from Tunisian hot spring water (40°C). The isolated diatom has been molecularly identified and classified into the genus Halamphora. The growth kinetics, lipid content and distribution of fatty acids were assessed at 20 and 30°C temperature levels and constant irradiance in controlled batch cultures (11 days). Halamphora sp. showed better growth (μ = 0.53 day−1) and a higher lipid yield (25% of the dry weight) at a higher temperature (30°C). Under the two temperatures tested, the highest lipid and fatty acid contents were mainly reached during the stationary growth phase. The fatty acid profile showed a significant content of two essential fatty acids, eicosapentaenoic acid (EPA, 20:5n-3) and arachidonic acid (AA, 20:4n-6), reaching ~15% and ~21% of the total fatty acids, respectively, at 20°C and 30°C. The distribution of the different components of the fatty acids showed that EPA and AA were mainly located in the neutral lipid fraction in the stationary phase.

Keywords

Arachidonic aciddiatomseicosapentaenoic acidfast growthHalamphoralipidsthermophile
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 100 , Issue 4 , June 2020 , pp. 529 - 536
Copyright
Copyright © Marine Biological Association of the United Kingdom 2020

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