Skip to main content Accessibility help
×
Home

Geographic variation in acid–base balance of the intertidal crustacean Cyclograpsus cinereus (Decapoda, Grapsidae) during air exposure

  • Marcelo Lagos (a1), Cristián W. Cáceres (a1) and Marco A. Lardies (a2)

Abstract

In intertidal poikilotherms with wide geographic distribution, physiological variations are ubiquitous, due to phenotypic plasticity and/or individual geographic variation. Using the grapsid crab, Cyclograpsus cinereus as a study model, acclimatization differences in respiratory physiology were evaluated among populations along the Chilean coast, covering a latitudinal gradient of about 2000 km. This species inhabits the supratidal zones and, therefore, is subject to constant immersion and emersion periods, producing physiological acidification due to CO2 retention, mainly in the branchial cavity. Individuals of six populations were collected along the coastline of Chile and were exposed to air for different time periods in the laboratory. The following parameters were measured: pH, Ca2+, Cl and haemolymphatic lactate dehydrogenase (LDH) enzyme activity. Populations from lower latitudes were significantly different from those from central and southern Chile, with a higher haemolymphatic pH variation and higher Ca2+ level, along with lower levels of Cl and LDH enzyme activity. This indicates that the populations from lower latitudes, which are subject to higher air temperatures during emersion, have a higher homeostatic capacity during emersion periods than those of intermediate and higher latitudes. This response seems to be determined by genetic bases due to adaptation to the local environment.

Copyright

Corresponding author

Correspondence should be addressed to: M.A. Lardies, Departamento de Ciencias, Facultad de Artes Liberales & Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibañez, Diagonal Las Torres 2640, Peñalolen, Santiago, Chile email: marco.lardies@uai.cl.

References

Hide All
Astete-Espinoza, L.P. and Cáceres, C.W. (2000) Efecto del parasitismo del isopodo bopirido Ionella agassizi (Isopoda: Epicaridea) (Bornier, 1900) sobre la fisiología nutricional del nape Neotrypaea uncinata (Edwards, 1837) (Decapoda: Thalassinidea). Revista Chilena de Historia Natural 73, 243252.
Bahamonde, N. and Lopez, M.T. (1969) Cyclograpsus cinereus Dana, en biocenosis supramareales de Chile (Crustacea Decapoda, Brachyura, Grapsidae). Boletín del Museo Nacional de Historia Natural 29, 165204.
Camus, P. (2001) Marine biogeography of continental Chile. Revista Chilena de Historia Natural 74, 587617.
Chen, J.C. and Chia, P.G. (1997) Osmotic and ionic concentrations of Scylla serrata (Forskål) subjected to different salinity levels. Comparative and Biochemical Physiology A 117, 239244.
Crawford, D.L. and Powers, D.A. (1992) Evolutionary adaptation to different thermal environments via transcriptional regulation. Molecular Biology and Evolution 9, 806813.
Fabry, V., Seibel, B., Feely, R. and Orr, J. (2008) Impacts of ocean acidification on marine fauna and ecosystem processes. ICES Journal of Marine Science 65, 414432.
Farrelly, C.A. and Greenaway, P. (1994) Gas exchange through the lungs and gills in air breathing crabs. Journal of Experimental Biology 187, 113130.
Fischer, K. and Fiedler, K. (2002) Reaction norms for age and size at maturity in response to temperature: a test of the compound interest hypothesis. Evolutionary Ecology 16, 333349.
Food and Agriculture Organization (1985) Datos agroclimatológicos: América Latina y El Caribe. Rome: Food and Agriculture Organization of the United Nations.
Gattuso, J.P. and Hansson, L. (2011) Ocean acidification. Oxford: Oxford University Press.
Greenaway, P. (2003) Terrestrial adaptation in the Anomura (Crustacea: Decapoda). Memoirs of the National Museum Victoria 60, 1326.
Gunthorpe, M.E., Sikes, C.S. and Wheeler, A.P. (1990) Promotion and inhibition of calcium carbonate crystallization in vitro by matrix protein from Blue Crab exoskeleton. Biological Bulletin. Marine Biological and Laboratory, Woods Hole 179, 191200.
Halperin, J., Ansaldo, M., Pellerano, G.N. and Luquet, C.M. (2000) Bimodal breathing in the estuarine crab Chasmagnathus granulatus Dana 1851, physiological and morfological studies. Comparative and Biochemical Physiology B 126, 341349.
Henry, R.P. (1994) Morphological, behavioral and physiological characterization of bimodal breathing crustaceans. American Zoologist 34, 205215.
Henry, R.P. (2001) Environmentally mediated carbonic anhydrase induction in the gills of euryhaline crustaceans. Journal of Experimental Biology 204, 9911002.
Henry, R.P. and Wheatly, M.G. (1992) Interaction of respiration, ion regulation, and acid–base balance in the everyday life of aquatic crustaceans. American Zoologist 32, 407416.
Henry, R.P., Kormanik, G.A., Smatresk, N.J. and Cameron, J.N. (1981) The role of CaCo3 dissolution as a source of HCO3 for the buffering of hypercapnic acidosis in aquatic and terrestrial decapod crustaceans. Journal of Experimental Biology 94, 269274.
Hochachka, P.W. and Somero, G.N. (2002) Biochemical adaptation. Oxford: Oxford University Press.
Hofmann, G.E. and Somero, G.N. (1995) Evidence for protein damage at environmental temperatures: seasonal changes in levels of ubiquitin conjugates and hsp70 in the intertidal mussel Mytilus trossulus. Journal of Experimental Biology 198, 15091518.
Innes, A.J., Forster, M.E., Jones, M.B., Marsden, I.D. and Taylor, H.H. (1986) Bimodal respiration, water balance and acid–base regulation in a high-shore crab, Cyclograpsus lavauxi. Journal of Experimental Marine Biology and Ecology 100, 127145.
Jensen, G.C. and Armstrong, D.A. (1991) Intertidal zonation among congeners: factors regulating distribution of Porcellan crabs Petrolisthes sp. (Anomura: Porcellanidae). Marine Ecology Progress Series 73, 4760.
Klin, Z. (1970) Empfehlungen der Deutschen Gesellschaft für Klinische, Chemie. Zeitschrift für klinische Chemie und klinische Biochemie 8, 658659.
Lagos, M.E. and Cáceres, C.W. (2008) Como afecta la exposición aérea el equilibrio ácido base de organismos móviles del intermareal: Petrolisthes laevigatus (Guérin, 1835) (Decapoda: Porcellanidae) como caso de estudio. Revista de Biología Marina y Oceanografía 43, 591598.
Lagos, M.E., Muñoz, J.L., Contreras, D.A. and Cáceres, C.W. (2011) Microhabitat segregation and physiological differences in two species of intertidal porcellanid crabs (genus Petrolisthes) on the southern coast of Chile. Scientia Marina 75, 273278.
Laptikhovsky, V. (2006) Latitudinal and bathymetric trends in egg size variation: a new look at Thorson's and Rass's rules. Marine Ecology 27, 714.
Lardies, M.A. and Bozinovic, F. (2006) Geographic covariation between metabolic rate and life-history traits. Evolutionary Ecology Research 8, 455470.
Lardies, M.A. and Bozinovic, F. (2008) Genetic variation for plasticity in physiological and life-history traits among populations of an invasive species, the terrestrial isopod Porcellio laevis. Evolutionary Ecology Research 10, 116.
Lardies, M.A. and Castilla, J.C. (2001) Latitudinal variation in the reproductive biology of the commensal crab Pinnaxodes chilensis (Decapoda: Pinnotheridae) along the Chilean coast. Marine Biology 139, 11251133.
Lardies, M.A., Bacigalupe, L.D. and Arias, M.B. (2010) Phenotypic covariance matrix in life history traits along a latitudinal gradient: a study case in an endemic crab from the coast of Chile. Marine Ecology Progress Series 412, 179187.
Lardies, M.A., Muñoz, J.L., Paschke, K.A. and Bozinovic, F. (2011) Geographic variation in metabolism in bimodal air–water breathing crab of Pacific coast of Chile: an evolutionary perspective. Marine Ecology: an Evolutionary Perspective 32, 4251.
Luquet, C. and Ansaldo, M. (1997) Acid–base balance and ionic regulation during emersion in the estuarine intertidal crab Chasmagnathus granulata Dana (Decapoda Grapsidae). Comparative and Biochemical Physiology A 117, 407410.
Mizera, F. and Meszéna, G. (2003) Spatial niche packing, character displacement and adaptative selection along an environmental gradient. Evolutionary Ecology Research 5, 363382.
Monaco, C.J., Brokordt, K. and Gaymer, C.F. (2010) Latitudinal thermal gradient effect on the cost of living of the intertidal porcelain crab Petrolisthes granulosus. Aquatic Biology 9, 2333.
Montecinos, L.A., Cisterna, J.A., Cáceres, C.W. and Saldías, G.S. (2009) Equilibrio ácido-base durante la exposición aérea en el molusco bivalvo Perumytilus purpuratus (Lamarck, 1819) (Bivalvia: Mytilidae). Revista de Biología Marina y Oceanografía 44, 181187.
Moorehead, W. and Biggs, H. (1974) 2-Amino-2-methyl-1-propanol as the alkalizing agent in an improved continous-flow cresolphthalein complexo procedure for calcium in serum. Clinical Chemistry 20, 14581480.
Moriyasu, M. and Mallet, P. (1986) Source molt stages of the snow crab Chionoecetes opilio by observation of morphogenesis of setae on the maxilla. Journal of Crustacean Biology 6, 709718.
Morris, S. (2002) The ecophysiology of air-breathing in crabs with special reference to Gecarcoidea natalis. Comparative and Biochemical Physiology B 131, 559570.
Morris, S. and Adamezwska, A.M. (1996) Respiratory, acid–base and ion status during voluntary immersion of the air-breathing crab Cardisoma carnifex assessed in situ. Journal of Experimental Marine Biology and Ecology 206, 149164.
O'Mahoney, P. and Full, R. (1984) Respiration of crabs in air and water. Comparative and Biochemical Physiology A 79, 275282.
Osovitz, C.J. and Hofmann, G.E. (2007) Marine macrophysiology: studying physiological variation across large spatial scales in marine systems. Comparative and Biochemical Physiology A 147, 821827.
Pane, E.F. and Barry, J.P. (2007) Extracellular acid–base regulation during short-term hypercapnia is effective in a shallow-water crab, but ineffective in a deep-sea crab. Marine Ecology Progress Series 334, 19.
Paynter, K., Dimichele, L. and Powers, D. (1991) Metabolic implications of Ldh-B genotype during early development in Fundulus heteroclitus. Journal of Experimental Biology 257, 2433.
Pellegrino, C. (1984) The role of desiccation pressures and surface area/volume relationships on seasonal zonation and size of four intertidal decapod crustacea from New Zealand: implication for adaptation to land. Crustaceana 47, 251268.
Pörtner, H.O., Bennett, A.F., Bozinovic, F., Clarke, A., Lardies, M.A., Lenski, R.E., Lucassen, M., Pelster, B., Shiemer, F. and Stillman, J.H. (2006) Trade-offs in thermal adaptation: the need for a molecular to ecological integration. Physiological and Biochemical Zoology 79, 295313.
Randall, D., Burggren, W. and French, K. (2001) Eckert, animal physiology: mechanisms and adaptations. New York; W.H. Freeman.
Ricklefs, R.E. and Wikelski, M. (2002) The physiology/life history nexus. Trends in Ecology and Evolution 17, 462468.
Schales, O. and Schales, S. (1941) A simple and accurate method for the determination of chloride in biological fluids. Journal of Biological Chemistry 140, 879.
Schulte, M.P., Glémet, H.C., Fiebig, A.A. and Powers, D.A. (2000) Adaptive variation in lactate dehydrogenase-B gene expression: role of a stress-responsive regulatory element. Proceedings of the National Academy of Sciences of the United States of America 97, 65976602.
SHOA (Servicio Hidrografico y Oceanografico de la Armada de Chile) (2006) Atlas Oceanografico de Chile, Vol. 1 (18°21′S a 50°00′S). Valparaiso: SHOA.
Sokal, R. and Rohlf, F. (1997) Biometry: The principles and practice of statistics in biological research. 3rd edition. New York: W.H. Freeman & Company, 887 pp.
Stillman, J.H. (2000) Evolutionary history and adaptive significance of respiratory structures on the legs of intertidal porcelain crabs. Physiological and Biochemical Zoology 73, 8696.
Thiel, M., Macaya, E., Acuña, E., Arntz, W., Bastías, H., Brokordt, K., Camus, P.A., Castilla, J.C., Castro, L.R., Cortés, M., Dumont, C.P., Escribano, R., Fernández, M., Lancelotti, D., Gajardo, J.A., Gaymer, C.F., Gómez, I., González, A.E., González, H.E., Haye, P.A., Illanes, J.E., Iriarte, J.L., Luna-Jorquera, G., Luxoro, C., Manríquez, P.H., Marín, V., Muñoz, P., Navarrete, S.A., Pérez, E., Poulin, E., Sellanes, J., Sepúlveda, A., Stotz, W., Tala, F., Thomas, A., Vargas, C.A., Vásquez, J.A. and Vega, A. (2007) The Humboldt Current System of northern and central Chile: oceanographic processes, ecological interactions and socioeconomic feedback. Oceanography and Marine Biology: an Annual Review 45, 195345.
Truchot, J.P. (1990) Respiratory and ionic regulation in invertebrates exposed to both water and air. Annual Review of Physiology 52, 6176.
Vargas, M.A., Lagos, M.E., Contreras, D.A. and Cáceres, C.W. (2010) Área de estructuras respiratorias y su efecto en la regulación del equilibrio ácido-base en dos especies de cangrejos porcelánidos intermareales, Petrolisthes laevigatus y Petrolisthes violaceus. Revista de Biología Marina y Oceanografía 45, 245253.
Varley, D. and Greenaway, P. (1992) The effect of emersion on hemolimph acid–base balance and oxygen levels in Silla serrata Forskål (Brachyura: Portunidae). Journal of Experimental Marine Biology and Ecology 163, 112.
Vernberg, F.J. (1959) Studies on the physiological variation between tropical and temperate zone fiddler crabs of the genus Uca. II. Oxygen consumption of whole organisms. Biological Bulletin. Marine Biological Laboratory, Woods Hole 117, 163184.
Waldron, F.M., Taylor, H.H. and Foster, M.E. (1986) Acid–base disturbances following exercise in a high-shore crab, Cyclograpsus lavauxi. New Zealand Journal of Marine and Freshwater Research 20, 479487.
Wheatly, M. and Henry, R. (1992) Extracellular and intracellular acid–base regulation in crustaceans. Journal of Experimental Zoology 263, 127142.
Whiteley, N.M., Rastrick, S.P.S., Lunt, D.H. and Rock, J. (2011) Latitudinal variations in the physiology of marine gammarid amphipods. Journal of Experimental Marine Biology and Ecology 400, 7077.
Willmer, P., Stone, G. and Johnston, I. (2000) Environmental physiology of animals. Oxford: Blackwell Science.
Yang, Z. and Bielanoski, J.P. (2000) Statistical methods for detecting molecular adaptation. Trends in Ecology and Evolution 15, 496503.
Zar, J. (1996) Bioestatistical analysis, 3rd edition. Upper Saddle River, NJ: Prentice-Hall.

Keywords

Geographic variation in acid–base balance of the intertidal crustacean Cyclograpsus cinereus (Decapoda, Grapsidae) during air exposure

  • Marcelo Lagos (a1), Cristián W. Cáceres (a1) and Marco A. Lardies (a2)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed