Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-23T09:31:34.189Z Has data issue: false hasContentIssue false
  • English
  • Français

The Component Combined Amino Acids of Some Marine Phytoplankton Species

Published online by Cambridge University Press:  11 May 2009

Y. K. Chau ,
L. Chuecas  and
J. P. Riley
Y. K. Chau
Affiliation:
Department of Oceanography, The University of Liverpool, Liverpool 3
L. Chuecas
Affiliation:
Department of Oceanography, The University of Liverpool, Liverpool 3
J. P. Riley
Affiliation:
Department of Oceanography, The University of Liverpool, Liverpool 3
Get access Rights & Permissions [Opens in a new window]

Extract

Gas-liquid and thin-layer chromatography have been used for the determination of 25 amino acids in the hydrolysates of 25 species of marine phytoplankton which had been grown in Erd-Schreiber medium. The general pattern of their distribution agrees with that found by earlier workers; the principal amino acids being glutamic acid, alanine, leucine and aspartic acids. Small amounts of 2-amino-n-butyric and 2-amino-iso-butyric acid together with compounds which have been tentatively identified as 4-amino-n-butyric acid and the 2-amino- and 2,5-diamino derivations of adipic acid were detected in many of the organisms. No differences were found which could be related to the taxonomy of the organisms.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 47 , Issue 3 , October 1967 , pp. 543 - 554
Copyright
Copyright © Marine Biological Association of the United Kingdom 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blau, K. & Darbre, A., 1963. Preparation of volatile derivatives of amino acids for gas chromatography. Biochem. J., Vol. 88, p. 8.Google Scholar
Brenner, M., Niederwieser, A., Pataki, G. & Weber, R., 1965. Theoretical aspects of thin-layer chromatography. In Thin Layer Chromatography, pp. 75–133 (ed. E., Stahl). New York: Academic Press.CrossRefGoogle Scholar
Citharel, J. 1966. Recherches sur les constituants azotés des algues marines. Les acides aminés libres. C. r. hebd. Séanc. Acad. Sci., Paris, Ser. D, Vol. 262, pp. 1495–7.Google Scholar
Cowey, C. B. & Corner, E. D. S. 1963. On the nutrition and metabolism of zoo-plankton. J. mar. biol. Ass. U.K., Vol. 43, pp. 495–511.Google Scholar
Cowey, C. B. & Corner, E. D. S., 1966. The amino-acid composition of certain unicellar algae and of the faecal pellets produced by Calanus finmarchicus when feeding on them. In Some Contemporary Studies in Marine Science, pp. 225–31 (ed. H., Barnes). London: George Allen and Unwin.Google Scholar
Cruickshank, P. A. & Sheehan, J. C., 1964. Gas chromatographic analysis of amino acids as N-trifluoroacetyl amino acid methyl esters. Analyt. Chem., Vol. 36, pp. 1191–7.CrossRefGoogle Scholar
El Khadem, H. S., El Shafei, Z. M. & Abdel, Rhaman M. M. A., 1963. Solvents for the paper chromatography of amino acids and sugars without spraying. Analyt. Chem., Vol. 35, pp. 1766–7.CrossRefGoogle Scholar
Fogg, G. E., 1953. The Metabolism of Algae, 149 pp. London: Methuen.CrossRefGoogle Scholar
Gehrke, C. W. & Shakrokhi, F. 1966. Chromatographic separation of butyl N-trifluoroacetyl esters of amino acids. Anal. Biochem., Vol. 15, pp. 97–109.CrossRefGoogle ScholarPubMed
Hong, S. W. (undated). On the amino acid patterns (sic) and its phylogenetic relationship in some marine green, brown and red. J. nat. Acad. Sci. Repub. Korea, Nat. Sci. Ser., pp. 185–205. Special Celebration Issue for Dr Fai-Choi Lee's 60th Birthday.Google Scholar
Ikekawa, N. 1963. Gas chromatography of amino acids. 1. Analysis of the mixture of methyl N-trifluoroacetyl derivatives. J. Biochem. Tokyo, Vol. 54, pp. 279–82.Google Scholar
Lambkin, W. M. & Gehrke, C. W., 1965. Quantitative gas chromatography of amino acids. Analyt. Chem., Vol. 37, pp. 383–9.CrossRefGoogle Scholar
Linko, P., Holm-Hansen, O., & Bassham, J. A., 1957. Formation of radioactive citrulline during photosynthetic C14O2 fixation by blue-green algae. J. exp. Bot., Vol. 8, pp. 147–56.Google Scholar
Low, E. M., 1955. Studies on some chemical constituents of diatoms. J. mar. Res., Vol. 14, pp. 199–204.Google Scholar
Magee, W. E. & Burris, R. H., 1954. Fixation of N2 and utilization of combined nitrogen by Nostoc muscorum. Am. J. Bot., Vol. 41, pp. 777–82.Google Scholar
Ogino, C., 1963. Studies on the chemical composition of some natural foods of aquatic animals, Bull. jap. Soc. Scient. Fish., Vol. 29, pp. 459–62.Google Scholar
Parke, M. & Dixon, P. S. 1964. A revised check-list of British marine algae. J. mar. biol. Ass. U.K., Vol. 44, pp. 499–542.Google Scholar
Parsons, T. R., Stephens, K. & Strickland, J. D. H., 1961. On the chemical composition of eleven species of marine phytoplankters. J. Fish. Res. Bd Can., Vol. 18, pp. 1001–16.Google Scholar
Round, F. E., 1965. The Biology of the Algae. 296 pp. London: Arnold.Google Scholar
Riley, J. P. & Wilson, T. R. S., 1967. The pigments of some marine phytoplankton species. J. mar. biol. Ass. U.K., Vol. 47 pp. 351–62.CrossRefGoogle Scholar
Rzhanova, G. N. & Goryunova, S. W., 1965. Amino acid composition of the blue-green alga Phormidium uncinatum. Mikrobiologiya, Vol. 34, pp. 268–72.Google Scholar
Serenkov, G. P. & Pakhomova, M. V., 1961. The chemical compounds of the green alga, Dunaliella salina. Vest. mosk. Univ. Biol. Pochvoved, Ser. 6, Vol. 16, pp. 22–6.Google Scholar
Silva, G. F., Etcheverry, D. H. & Quilhot, P. W., 1965. Determination cromatografica de amino-acidos en algas marinas. Bot. mar., Vol. 8, pp. 244–51.CrossRefGoogle Scholar
Smith, D. G. & Young, E. G., 1956. The combined amino acids in several species of marine algae. J. biol. Chem., Vol. 217, pp. 845–53.CrossRefGoogle Scholar
Stalling, D. L. & Gehrke, C. W., 1966. Quantitative analysis of amino acids by gas chromatography: acylation of arginine. Biochem. biophys. Res. Commun. Vol. 22, pp. 329–35.Google Scholar
Strickland, J. D. H., 1965. Production of organic matter in the primary stages of the marine food chain. Chemical Oceanography Vol. 1. pp. 477–610 (eds Riley, J. P. and G., Skirrow) London: Academic Press.Google Scholar
Teuwissen, B., Lenain, C., Dorlet, C. & Léonis, J., 1962. Separation of amino acids by gas chromatography. Proc. 2nd Chromatog. Symp., Brussels, pp. 81–6.Google Scholar