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On the nutrition and metabolism of zooplankton XII. Measurements by radioimmunoassay of the levels of a steroid in Calanus

Published online by Cambridge University Press:  11 May 2009

S. C. M. O'Hara ,
E. D. S. Corner  and
C. C. Kilvington
S. C. M. O'Hara
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
E. D. S. Corner
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
C. C. Kilvington
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
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Extract

A steroid that binds with mammalian oestradiol-17β antibody has been detected in Calanus helgolandicus Claus, collected from Plymouth Sound, and in Calanus finmarchicus Gunnerus captured off the coast of Norway. The specificity of the antibody, together with the chromatographic behaviour of the steroid on Sephadex LH-20, supports the view that the compound is the sex hormone oestradiol-17β (E2). The antibody has been used in measuring the amounts of E2 in the animals by radioimmunoassay, a technique not previously employed in studies with zooplankton and therefore described in detail.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 58 , Issue 3 , August 1978 , pp. 597 - 605
Copyright
Copyright © Marine Biological Association of the United Kingdom 1978

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