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Tinospora cordifolia consumption ameliorates changes in kidney chondroitin sulphate/dermatan sulphate in diabetic rats

  • Darukeshwara Joladarashi (a1), Nandini D. Chilkunda (a1) and Paramahans V. Salimath (a1)

Abstract

Diabetes is known to alter kidney extracellular matrix (ECM) components. Chondroitin sulphate (CS)/dermatan sulphate (DS), an ECM component, which plays an essential role in kidney is altered during diabetes. The focus of this study has been to examine the effect of Tinospora cordifolia (TC) consumption, a potent plant widely used to treat diabetes, on kidney CS/DS. Experimentally induced diabetic rats were fed with diet containing TC at 2·5 and 5 % levels and the effect of it on kidney CS/DS was examined. The CS/DS content and CS:heparan sulphate ratio which was decreased during diabetic condition were ameliorated in TC-fed groups. Disaccharide composition analysis of CS/DS by HPLC showed that decreases in ‘E’ units and degree of sulphation were modulated in 5 % TC-fed groups. Apparent molecular weight of purified CS/DS from the control rat kidney was found to be 38 kDa which was decreased to 29 kDa in diabetic rat kidney. Rats in 5 % TC-fed groups showed chain length of 38 kDa akin to control rats. Expression of chondroitin 4-O-sulfotransferase-1, dermatan 4-O-sulfotransferase-1 and N-acetylgalactosamine 4 sulphate 6-O-sulfotransferase, enzymes involved in the synthesis of ‘E’ units which was reduced during diabetic condition, was significantly contained in the 5 % TC-fed group. Purified CS/DS from 5 % TC-fed group was able to bind higher amounts of ECM components, namely type IV collagen and laminin, when compared with untreated diabetic rats. The present results demonstrate that consumption of a diet containing TC at the 5 % level modulates changes in kidney CS/DS which were due to diabetes.

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Corresponding author

*Corresponding author: Dr P. V. Salimath, fax +91 821 2517233, email salimath1954@gmail.com

References

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1.Fowler, MJ (2008) Microvascular and macrovascular complications of diabetes. Clin Diab 26, 7782.
2.Zelmanovitz, T, Gerchman, F, Balthazar, AP, et al. (2009) Diabetic nephropathy. Diabetol Metab Syndr 21, 110.
3.Maxhimer, JB, Somenek, M, Rao, G, et al. (2005) Heparanase-1 gene expression and regulation by high glucose in renal epithelial cells: a potential role in the pathogenesis of proteinuria in diabetic patients. Diabetes 54, 21722178.
4.Shimomura, H & Spiro, R (1987) Studies on macromolecular components of human glomerular basement membrane and alterations in diabetes: decreased levels of heparan sulfate proteoglycans and laminin. Diabetes 36, 374381.
5.Mason, RM & Wahab, NA (2003) Extracellular matrix metabolism in diabetic nephropathy. J Am Soc Nephrol 14, 13581373.
6.Silbert, JE & Sugumaran, G (2002) Biosynthesis of chondroitin/dermatan sulfate. IUBMB Life 54, 177186.
7.Joladarashi, D, Salimath, PV & Nandini, CD (2011) Diabetes results in structural alteration of chondroitin sulfate/dermatan sulfate in the rat kidney: effects on the binding to extracellular matrix components. Glycobiology 21, 960972.
8.Steer, DL, Shah, MM, Bush, KT, et al. (2004) Regulation of ureteric bud branching morphogenesis by sulfated proteoglycans in the developing kidney. Dev Biol 272, 310327.
9.Jeansson, M & Haraldsson, B (2003) Glomerular size and charge selectivity in the mouse after exposure to glucosaminoglycan-degrading enzymes. J Am Soc Nephrol 14, 17561765.
10.Haneda, M, Koya, D, Isono, M, et al. (2003) Overview of glucose signalling in mesangial cells in diabetic nephropathy. J Am Soc Nephrol 14, 13741382.
11.Puranik, NK, Kammar, KF & Sheela, DR (2010) Anti-diabetic activity of Tinospora cordifolia (Willd.) in streptozotocin diabetic rats; does it act like sulfonylureas? Turk J Med Sci 40, 265270.
12.Gray, AM & Flatt, PR (1997) Nature's own pharmacy: the diabetes perspective. Proc Nutr Soc 56, 507517.
13.Suresh Kumara, G, Shetty, AK & Salimath, PV (2008) Modulatory effect of bitter gourd (Momordica charantia LINN.) on alterations in kidney heparan sulfate in streptozotocin-induced diabetic rats. J Ethnopharmacol 115, 276283.
14.Kameswararao, B, Kesavulu, MM & Apparao, C (2003) Evaluation of antidiabetic effect of Momordica cymbalaria fruit in alloxan-diabetic rats. Fitotherapia 74, 713.
15.Cencic, A & Chingwaru, W (2010) The role of functional foods, nutraceuticals, and food supplements in intestinal health. Nutrients 2, 611625.
16.Nandini, CD, Sambaiah, K & Salimath, PV (2003) Dietary fibres ameliorate decreased synthesis of heparan sulfate in streptozotocin induced diabetic rats. J Nutr Biochem 14, 203210.
17.Kumar, PA, Nandini, CD & Salimath, PV (2011) Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid. FEBS J 278, 143155.
18.Chopra, RN, Chopra, LC, Handa, KD, et al. , editors (1982) Indigenous Drugs of India, 2nd ed., pp. 426428. Kolkota: M/S Dhar VN & Sons.
19.Gupta, SS, Verma, SC, Garg, VP, et al. (1967) Antidiabetic effects of Tinospora cordifolia. Part 1. Effect on fasting blood sugar level, glucose tolerance and adrenaline induced hyperglycaemia. Indian J Med Res 55, 733745.
20.Atal, CK, Sharma, ML & Kaul, A (1986) Immunomodulating agents of plant origin. I: preliminary screening. J Ethnopharmacol 18, 133141.
21.Peer, F & Sharma, MC (1989) Therapeutic evaluation of Tinospora cordifolia in CCl4 induced hepatopathy in goats. Indian J Vet Med 9, 154156.
22.Vedavathy, S & Rao, KN (1991) Antipyretic activity of six indigenous medicinal plants of Tirumala Hills Andhra Pradesh, India. J Ethnopharmacol 33, 12.
23.Bieri, JG, Stoewsand, GS, Briggs, GM, et al. (1997) Report of the American institute of nutrition, ad hoc committee on standards for nutritional studies. J Nutr 107, 13401348.
24.Hatch, GM, Cao, SG & Angel, A (1995) Decrease in cardiac phosphatidyl glycerol in streptozotocin induced diabetic rats does not affect cardiolipin biosynthesis. Evident for distant pools of phospolipid glycerol in the heart. Biochem J 306, 759764.
25.Bowers, LD (1980) Kinetic serum creatinine assays I. The role of various factors in determining specificity. Clin Chem 26, 551554.
26.Yokozawa, T, Chung, HY, He, LQ, et al. (1996) Effectiveness of green tea tannin on rats with chronic renal failure. Biosci Biotechnol Biochem 60, 10001005.
27.Kessler, MA, Meinitzer, A, Petek, W, et al. (1997) Microalbuminuria and borderline-increased albumin excretion determined with a centrifugal analyzer and the Albumin Blue 580 fluorescence assay. Clin Chem 43, 9961002.
28.Scott, JE (1960) Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues. Methods Biochem Anal 8, 145197.
29.Shively, JE & Conrad, HE (1976) Formation of anhydrosugars in the chemical depolymerization of heparin. Biochemistry 15, 39323942.
30.Chandrasekhar, S, Esterman, MA & Hoffman, H (1987) A microdetermination of proteoglycan and glycosaminoglycans in the presence of guanidine hydrochloride. Anal Biochem 161, 103108.
31.Saito, H, Yamagata, T & Suzuki, S (1968) Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates. J Biol Chem 243, 15361542.
32.Kinoshita, A & Sugahara, K (1999) Microanalysis of glycosaminoglycan-derived oligosaccharides labeled with a fluorophore 2-aminobenzamide by high-performance liquid chromatography: Application to disaccharide composition analysis and exosequencing of oligosaccharides. Anal Biochem 269, 367378.
33.Bigge, JC, Patel, TP, Bruce, JA, et al. (1995) Non-selective and efficient fluorescent labelling of glycans using 2-aminobenzamide and anthranilic acid. Anal Biochem 230, 229238.
34.Farndale, RW, Buttle, DJ & Barrett, AJ (1986) Improved quantitation and discrimination of sulfated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883, 173177.
35.Hugget, ASG & Nixon, DA (1957) Use of glucose oxidase, peroxidase and O-dianisidine in the determination of blood glucose and urinary glucose. Lancet 273, 366370.
36.Miller, GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31, 426428.
37.Nandini, CD, Mikami, T, Ohta, M, et al. (2004) Structural and functional characterization of oversulfated chondroitin sulfate/dermatan sulfate hybrid chains from the notochord of hagfish neuritogenic and binding activities for growth factors and neurotrophic factors. J Biol Chem. 279, 5079950809.
38.Sakai, S, Kim, WS, Lee, IS, et al. (2003) Purification and characterization of dermatan sulfate from the skin of the eel, Anguilla japonica. Carbohydr Res 31, 263269.
39.Bechard, D, Gentina, T, Delehedde, M, et al. (2001) Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem 21, 4834148349.
40.Rajalakshmi, M, Eliza, J, Priya, CE, et al. (2009) Anti-diabetic properties of Tinospora cordifolia stem extracts on streptozotocin-induced diabetic rats. Afr J Pharm Pharmacol 3, 171180.
41.Purushothaman, A, Fukuda, J, Mizumoto, S, et al. (2007) Functions of chondroitin sulfate/dermatan sulfate chains in brain development. Critical roles of E and iE disaccharide units recognized by a single chain antibody GD3G7. J Biol Chem 282, 1944219452.
42.Deepa, SS, Umehara, Y, Higashiyam, S, et al. (2002) Specific molecular interactions of oversulfated chondroitin sulfate E with various heparin-binding growth factors. Implications as a physiological binding partner in the brain and other tissues. J Biol Chem 277, 4370743716.
43.Schleipen, B, Hertrampf, T, Fritzemeier, K, et al. (2011) ER {beta}-specific agonists and genistein inhibit proliferation and induce apoptosis in the large and small intestine. Carcinogenesis 11, 16751683.
44.Bhawya, D & Anilkumar, KR (2010) In vitro antioxidant potency of Tinospora cordifolia (gulancha) in sequential extracts. IJPBA 5, 448456.
45.Panchabhai, TS, Kulkarni, UP & Rege, NN (2008) Validation of therapeutic claims of Tinospora cordifolia: a review. Phytother Res 22, 425441.
46.Paganga, G & Rice-Evans, CA (1997) The identification of flavanoids as glycosides in human plasma. FEBS Lett 13, 7882.
47.Wu, X, Cao, G & Prior, RL (2002) Absorption and metabolism of anthocyanins in human subjects following consumption of elderberry or blueberry. J Nutr 132, 18651871.
48.Latha, MS, Vijayammal, PL & Kurup, PA (1991) Charges in the glycosaminoglycans and glycoproteins in the tissues in rats exposed to cigarette smoking. Atherosclerosis 31, 4954.
49.Volpi, N & Tarugi, P (1999) Influence of chondroitin sulfate charge density, sulfate group position and molecular mass on Cu+2 mediated oxidation of human low density lipoproteins: effect of normal human plasma derived chondroitin sulfate. J Biol Chem 125, 297304.
50.Kalea, AZ, Lamari, FN, Theocharis, AD, et al. (2006) Wild blueberry (Vaccinium angustifolium) consumption affects the composition and structure of glycosaminoglycans in Sprague–Dawley rat aorta. J Nutr Biochem 17, 109116.
51.Ivanov, V, Ivanova, S, Kalinovsky, T, et al. (2008) Plant-derived micronutrients suppress monocyte adhesion to cultured human aortic endothelial cell layer by modulating its extracellular matrix composition. J Cardiovasc Pharmacol 52, 5565.
52.Smith, JG, Yokoyama, WH & German, JB (2000) Butyric acid from the diet: actions at the level of gene expression. Crit Rev Food Sci 38, 259297.
53.Karlsen, AE, Fujimoto, WY, Rabinovitch, P, et al. (1991) Effects of sodium butyrate on proliferation-dependent insulin gene expression and insulin release in glucose-sensitive RIN-5AH cells. J Biol Chem 266, 75427548.
54.Gao, Z, Yin, J, Zhang, J, et al. (2009) Butyrate improves insulin sensitivity and increases energy expenditure in mice. Diabètes 58, 15091517.

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