Skip to main content Accessibility help
×
Home

Evaluation of an in vitro fibre fermentation method using feline faecal inocula: repeatability and reproducibility

  • Guido Bosch (a1), Lisa Heesen (a1), Karine de Melo Santos (a1), Wilbert F. Pellikaan (a1), John W. Cone (a1) and Wouter H. Hendriks (a1) (a2)...

Abstract

To gain knowledge on the precision of an in vitro method for characterisation of the fermentability of dietary fibres, this study aimed to evaluate the repeatability and reproducibility of such a method. Substrates used were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from five cats with three replicates for each substrate–cat combination. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) were determined after incubation. Four consecutive runs were performed. The within-run variability (repeatability) was generally lower for the more simple and pure substrates (CP, FOS, GG) than for the more complex substrates containing mixtures of fibres (SBP, WM). Replicates showed high variability, in particular for SCFA profiles and parameters of gas production kinetics. The between-run CV (reproducibility) for the measured parameters were, in general, below 10 % for CP, FOS and GG and higher values were obtained for SBP and WM. It is concluded that for precise dietary fibre characterisation, the number of replicates should be multiple and adjusted according to the variability of the parameters of interest and the complexity of fibres. The method yielded reproducible results with some variation in absolute values obtained, which may have an impact on the significance level of the differences among substrates.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Evaluation of an in vitro fibre fermentation method using feline faecal inocula: repeatability and reproducibility
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Evaluation of an in vitro fibre fermentation method using feline faecal inocula: repeatability and reproducibility
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Evaluation of an in vitro fibre fermentation method using feline faecal inocula: repeatability and reproducibility
      Available formats
      ×

Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

* Corresponding author: G. Bosch, email guido.bosch@wur.nl

References

Hide All
1. Fahey, GC Jr, Flickinger, AE, Grieshop, CM, et al. (2004) The role of dietary fibre in companion animal nutrition. In Dietary Fibre: Bio-Active Carbohydrates for Food and Feed, pp. 295328 [Van der Kamp, JW, Asp, N-G, Miller Jones, J and Schaafsma, G, editors]. Wageningen, The Netherlands: Wageningen Academic Publishers.
2. Coles, LT, Moughan, PJ & Darragh, AJ (2005) In vitro digestion and fermentation methods, including gas production techniques, as applied to nutritive evaluation of foods in the hindgut of humans and other simple-stomach animals. Anim Feed Sci Technol 123–124, 421444.
3. Bosch, G, Heesen, L, de Melo Santos, K, et al. (2017) Evaluation of an in vitro fibre fermentation method using feline faecal inocula: inter-individual variation. J Nutr Sci 6, doi:10.1017/jns.2017.21.
4. Van Laar, H, Van Straalen, WM, Van Gelder, AH, et al. (2006) Repeatability and reproducibility of an automated gas production technique. Anim Feed Sci Technol 127, 133150.
5. Flickinger, EA & Fahey, GC Jr (2002) Pet food and feed applications of inulin, oligofructose and other oligosaccharides. Br J Nutr 87, S297S300.
6. Karr-Lilienthal, LK, Grieshop, CM, Smeets-Peeters, MJE, et al. (2002) Selected gelling agents in canned dog food affect nutrient digestibilities and fecal characteristics of ileal cannulated dogs. Arch Anim Nutr 56, 141153.
7. Barry, KA, Wojcicki, BJ, Bauer, LL, et al. (2011) Adaptation of healthy adult cats to select dietary fibers in vivo affects gas and short-chain fatty acid production from fiber fermentation in vitro . J Anim Sci 89, 31633169.
8. Van Rooijen, C, Bosch, G, Butré, CI, et al. (2016) Urinary excretion of dietary Maillard reaction products in healthy adult female cats. J Anim Sci 94, 185195.
9. Williams, BA, Bosch, MW, Boer, H, et al. (2005) An in vitro batch culture method to assess potential fermentability of feed ingredients for monogastric diets. Anim Feed Sci Technol 123–124, 445462.
10. Cone, JW, Van Gelder, AH, Visscher, GJW, et al. (1996) Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus. Anim Feed Sci Technol 61, 113128.
11. Peachey, SE, Dawson, JM & Harper, EJ (2000) Gastrointestinal transit times in young and old cats. Comp Biochem Physiol A 126, 8590.
12. Papasouliotis, K, Sparkes, AH, Gruffydd-Jones, TJ, et al. (1998) Use of the breath hydrogen test to assess the effect of age on orocecal transit time and carbohydrate assimilation in cats. Am J Vet Res 59, 12991302.
13. Bosch, G, Pellikaan, WF, Rutten, PGP, et al. (2008) Comparative in vitro fermentation activity in the canine distal gastrointestinal tract and fermentation kinetics of fiber sources. J Anim Sci 86, 29792989.
14. Groot, JCJ, Cone, JW, Williams, BA, et al. (1996) Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds. Anim Feed Sci Technol 64, 7789.
15. Bauer, E, Williams, BA, Voigt, C, et al. (2003) Impact of mammalian pretreatment on the fermentability of carbohydrate-rich feedstuffs. J Sci Food Agric 83, 207214.
16. Lahti, L, Salojärvi, J, Salonen, A, et al. (2014) Tipping elements in the human intestinal ecosystem. Nat Commun 5, 4344.
17. McBurney, MI & Thompson, LU (1987) Effect of human faecal donor on in vitro fermentation variables. Br J Nutr 58, 233243.

Keywords

Evaluation of an in vitro fibre fermentation method using feline faecal inocula: repeatability and reproducibility

  • Guido Bosch (a1), Lisa Heesen (a1), Karine de Melo Santos (a1), Wilbert F. Pellikaan (a1), John W. Cone (a1) and Wouter H. Hendriks (a1) (a2)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed