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Repair of dentin defects from DSPP knockout mice by PILP mineralization

  • Hamid Nurrohman (a1), Kunkio Saeki (a1), Karina M.M. Carneiro (a1), Yung-Ching Chien (a1), Sabra Djomehri (a1), Sunita P. Ho (a1), Chunlin Qin (a2), Laurie B. Gower (a3), Sally J. Marshall (a4), Grayson W. Marshall (a4) and Stefan Habelitz (a4)...

Abstract

Dentinogenesis imperfecta type II (DGI-II) lacks intrafibrillar mineral with severe compromise of dentin mechanical properties. A Dspp knockout (Dspp –/–) mouse, with a phenotype similar to that of human DGI-II, was used to determine if poly-L-aspartic acid [poly(ASP)] in the “polymer-induced liquid-precursor” (PILP) system can restore its mechanical properties. Dentin from six-week old Dspp –/– and wild-type mice was treated with CaP solution containing poly(ASP) for up to 14 days. Elastic modulus and hardness before and after treatment were correlated with mineralization from Micro x-ray computed tomography (Micro-XCT). Transmission electron microscopy (TEM)/Selected area electron diffraction (SAED) were used to compare matrix mineralization and crystallography. Mechanical properties of the Dspp –/– dentin were significantly less than wild-type dentin and recovered significantly (P < 0.05) after PILP-treatment, reaching values comparable to wild-type dentin. Micro-XCT showed mineral recovery similar to wild-type dentin after PILP-treatment. TEM/SAED showed repair of patchy mineralization and complete mineralization of defective dentin. This approach may lead to new strategies for hard tissue repair.

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a) Address all correspondence to this author. e-mail: Stefan.Habelitz@ucsf.edu

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Contributing Editor: Michelle L. Oyen

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References

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1. Kinney, J.H., Pople, J.A., Driessen, C.H., Breunig, T.M., Marshall, G.W., and Marshall, S.J.: Intrafibrillar mineral may be absent in dentinogenesis imperfecta type II (DI-II). J. Dent. Res. 80(6), 1555 (2001).
2. Kinney, J.H., Habelitz, S., Marshall, S.J., and Marshall, G.W.: The importance of intrafibrillar mineralization of collagen on the mechanical properties of dentin. J. Dent. Res. 82(12), 957 (2003).
3. Deshpande, A.S., Fang, P.A., Zhang, X., Jayaraman, T., Sfeir, C., and Beniash, E.: Primary structure and phosphorylation of dentin matrix protein 1 (DMP1) and dentin phosphophoryn (DPP) uniquely determine their role in biomineralization. Biomacromolecules 12(8), 2933 (2011).
4. Gibson, M.P., Zhu, Q., Liu, Q., D'Souza, R.N., Feng, J.Q., and Qin, C.: Loss of dentin sialophosphoprotein leads to periodontal diseases in mice. J. Periodontal Res. 48(2), 221 (2013).
5. Kim, J.W., Nam, S.H., Jang, K.T., Lee, S.H., Kim, C.C., Hahn, S.H., Hu, J.C., and Simmer, J.P.: A novel splice acceptor mutation in the DSPP gene causing dentinogenesis imperfecta type II. Hum. Genet. 115(3), 248 (2004).
6. Kim, J.W., Hu, J.C., Lee, J.I., Moon, S.K., Kim, Y.J., Jang, K.T., Lee, S.H., Kim, C.C., Hahn, S.H., and Simmer, J.P.: Mutational hot spot in the DSPP gene causing dentinogenesis imperfecta type II. Hum. Genet. 116(3), 186 (2005).
7. Sreenath, T., Thyagarajan, T., Hall, B., Longenecker, G., D'Souza, R., Hong, S., Wright, J.T., MacDougall, M., Sauk, J., and Kulkarni, A.B.: Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis imperfecta type III. J. Biol. Chem. 278(27), 24874 (2003).
8. Olszta, M.J., Odom, D.J., Douglas, E.P., and Gower, L.B.: A new paradigm for biomineral formation: Mineralization via an amorphous liquid-phase precursor. Connect. Tissue Res. 44(Suppl 1), 326 (2003).
9. Gower, L.B.: Biomimetic model systems for investigating the amorphous precursor pathway and its role in biomineralization. Chem. Rev. 108(11), 4551 (2008).
10. Tay, F.R. and Pashley, D.H.: Guided tissue remineralisation of partially demineralised human dentine. Biomaterials 29(8), 1127 (2008).
11. Nudelman, F., Pieterse, K., George, A., Bomans, P.H., Friedrich, H., Brylka, L.J., Hilbers, P.A., de with, G., and Sommerdijk, N.A.: The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors. Nat. Mater. 9(12), 1004 (2010).
12. Nurrohman, H., Nakashima, S., Takagaki, T., Sadr, A., Nikaido, T., Asakawa, Y., Uo, M., Marshall, S.J., and Tagami, J.: Immobilization of phosphate monomers on collagen induces biomimetic mineralization. Bio-med. Mater. Eng. 25(1), 89 (2015).
13. Habelitz, S., Marshall, G.W. Jr., Balooch, M., and Marshall, S.J.: Nanoindentation and storage of teeth. J. Biomech. 35(7), 995 (2002).
14. Burwell, A.K., Thula-Mata, T., Gower, L.B., Habelitz, S., Kurylo, M., Ho, S.P., Chien, Y.C., Cheng, J., Cheng, N.F., Gansky, S.A., Marshall, S.J., and Marshall, G.W.: Functional remineralization of dentin lesions using polymer-induced liquid-precursor process. PLoS One 7(6), e38852 (2012).
15. Habelitz, S., Balooch, M., Marshall, S.J., Balooch, G., and Marshall, G.W. Jr.: In situ atomic force microscopy of partially demineralized human dentin collagen fibrils. J. Struct. Biol. 138(3), 227 (2002).
16. Bertassoni, L.E., Habelitz, S., Marshall, S.J., and Marshall, G.W.: Mechanical recovery of dentin following remineralization in vitro—An indentation study. J. Biomech. 44(1), 176 (2011).
17. Djomehri, S.I., Candell, S., Case, T., Browning, A., Marshall, G.W., Yun, W., Lau, S.H., Webb, S., and Ho, S.P.: Mineral density volume gradients in normal and diseased human tissues. PLoS One 10(4), e0121611 (2015).
18. Nurrohman, H., Nikaido, T., Takagaki, T., Sadr, A., Ichinose, S., and Tagami, J.: Apatite crystal protection against acid-attack beneath resin-dentin interface with four adhesives: TEM and crystallography evidence. Dent. Mater. 28(7), e89 (2012).
19. Zavgorodniy, A.V., Rohanizadeh, R., and Swain, M.V.: Ultrastructure of dentine carious lesions. Arch. Oral Biol. 53(2), 124 (2008).
20. Thula-Mata, T., Burwell, A., Gower, L.B., Habeliz, S., and Marshall, G.W.: Remineralization of artificial dentin lesions via the polymer-induced liquid-precursor (PILP) process. Mater. Res. Soc. Symp. Proc. 1355, 1114 (2011).
21. Habelitz, S., Hsu, T., Hsiao, P., Saeki, K., Chien, Y.C., Marshall, S.J., and Marshall, G.W.: The natural process of biomineralization and in-vitro remineralization of dentin lesions. 1. Advances in bioceramics and biotechnologies II. Ceram. Trans. 247, 13 (2014).
22. Balooch, M., Habelitz, S., Kinney, J.H., Marshall, S.J., and Marshall, G.W.: Mechanical properties of mineralized collagen fibrils as influenced by demineralization. J. Struct. Biol. 162(3), 404 (2008).
23. Bertassoni, L.E., Habelitz, S., Kinney, J.H., Marshall, S.J., and Marshall, G.W. Jr.: Biomechanical perspective on the remineralization of dentin. Caries Res. 43(1), 70 (2009).
24. Levin, L.S., Leaf, S.H., Jelmini, R.J., Rose, J.J., and Rosenbaum, K.N.: Dentinogenesis imperfecta in the brandywine isolate (DI type III): Clinical, radiologic, and scanning electron microscopic studies of the dentition. Oral Surg., Oral Med., Oral Pathol. 56(3), 267 (1983).
25. Fang, P.A., Verdelis, K., Yang, X., Lukashova, L., Boskey, A.L., and Beniash, E.: Ultrastructural organization of dentin in mice lacking dentin sialo-phosphoprotein. Connect. Tissue Res. 55(Suppl 1), 92 (2014).
26. Sun, J., Chen, C., Pan, H., Chen, Y., Mao, C., Wang, W., Tang, R., and Gu, X.: Biomimetic promotion of dentin remineralization using L-glutamic acid: Inspiration from biomineralization proteins. J. Mater. Chem. B 2, 4544 (2014).

Keywords

Repair of dentin defects from DSPP knockout mice by PILP mineralization

  • Hamid Nurrohman (a1), Kunkio Saeki (a1), Karina M.M. Carneiro (a1), Yung-Ching Chien (a1), Sabra Djomehri (a1), Sunita P. Ho (a1), Chunlin Qin (a2), Laurie B. Gower (a3), Sally J. Marshall (a4), Grayson W. Marshall (a4) and Stefan Habelitz (a4)...

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