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One-pot solvothermal method to prepare functionalized Fe3O4 nanoparticles for bioseparation

  • Guoxin Zhang (a1), Fengxiang Qie (a2), Jianxuan Hou (a2), Shizhong Luo (a2), Liang Luo (a3), Xiaoming Sun (a3) and Tianwei Tan (a4)...

Abstract

Surface-functionalized magnetic nanoparticles were prepared by a facile one-pot solvothermal method in ethylene glycol solution. Zeta value, size, and magnetic properties could be well tuned by introducing different functional group molecules. Characterizations, including transmission electronic microscopy, scanning electronic microscopy, thermogravimetric analysis, x-ray powder diffraction and vibrating sample magnetometer, and Fourier transform infrared spectrophotometer demonstrated the efficiency of this simple and general synthesis strategy. The hydrophilic magnetic nanoparticles with various surface functional groups and zeta values were evidenced as excellent candidates for bioseparation by extracting DNA molecules from a model mixture of cell fractures.

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a)Address all correspondence to these authors. e-mail: sunxm@mail.buct.edu.cn

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1.Son, S.J., Reichel, J., He, B., Schuchman, M., and Lee, S.B.: Magnetic nanotubes for magnetic-field-assisted bioseparation, biointeraction, and drug delivery. J. Am. Chem. Soc. 127(20), 7316 (2005).
2.Fang, W., Chen, X., and Zheng, N.: Superparamagnetic core-shell polymer particles for efficient purification of his-tagged proteins. J. Mater. Chem. 20(39), 8624 (2010).
3.Chun, J., Seo, S.W., Jung, G.Y., and Lee, J.: Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters. J. Mater. Chem. 21(18), 6713 (2011).
4.Lu, A-H., Salabas, E.L., and Schüth, F.: Magnetic nanoparticles: Synthesis, protection, functionalization, and application. Angew. Chem. Int. Ed. 46(8), 1222 (2007).
5.Berensmeier, S.: Magnetic particles for the separation and purification of nucleic acids. Appl. Microbiol. Biotechnol. 73(3), 495 (2006).
6.Xu, R., Sun, G., Li, Q., Wang, E., and Gu, J.: A dual-responsive superparamagnetic Fe3O4/Silica/PAH/PSS material used for controlled release of chemotherapeutic agent, keggin polyoxotungstate, PM-19. Solid State Sci. 12, 1720 (2010).
7.Chen, D., Jiang, M., Li, N., Gu, H., Xu, Q., Ge, J., Xia, X., and Lu, J.: Modification of magnetic silica/iron oxide nanocomposites with fluorescent polymethacrylic acid for cancer targeting and drug delivery. J. Mater. Chem. 20(31), 6422 (2010).
8.Xu, Z., Feng, Y., Liu, X., Guan, M., Zhao, C., and Zhang, H.: Synthesis and characterization of Fe3O4@SiO2@poly-l-alanine, peptide brush-magnetic microspheres through NCA chemistry for drug delivery and enrichment of BSA. Colloids Surf. B 81, 503 (2010).
9.Luo, B., Xu, S., Luo, A., Wang, W.R., Wang, S.L., Guo, J., Lin, Y., Zhao, D.Y., and Wang, C.C.: Mesoporous biocompatible and acid-degradable magnetic colloidal nanocrystal clusters with sustainable stability and high hydrophobic drug loading capacity. ACS Nano 5(2), 1428 (2011).
10.Govindaiah, P., Park, T.J., Jung, Y.J., Lee, S.J., Ryu, D.Y., Kim, J.H., and Cheong, I.W.: Luminescent iron oxide nanoparticles prepared by one-pot aphen-functionalization. Macromol. Res. 18(11), 1109 (2010).
11.Lin, M.M., Li, S., Kim, H.H., Kim, H., Lee, H.B., and Muhammed, M.: Complete separation of magnetic nanoparticles via chemical cleavage of dextran by ethylenediamine for intracellular uptake. J. Mater. Chem. 20(3), 444 (2009).
12.Liu, J., Sun, Z., Deng, Y., Zou, Y., Li, C., Guo, X., Xiong, L., Gao, Y., Li, F., and Zhao, D.: Highly water-dispersible biocompatible magnetite particles with low cytotoxicity stabilized by citrate groups. Angew. Chem. Int. Ed. 121(32), 5989 (2009).
13.Oh, J.K. and Park, J.M.: Iron oxide-based superparamagnetic polymeric nanomaterials: Design, preparation, and biomedical application. Prog. Polym. Sci. 36, 168 (2011).
14.Xuan, S., Wang, F., Lai, J.M.Y., Sham, K.W.Y., Wang, Y.X.J., Lee, S.F., Yu, J.C., Cheng, C.H.K., and Leung, K.C.F.: Synthesis of biocompatible, mesoporous Fe3O4 nano/microspheres with large surface area for magnetic resonance imaging and therapeutic applications. ACS Appl. Mater. Interfaces 3, 237 (2011).
15.Shi, Z., Neoh, K.G., Kang, E.T., Shuter, B., Wang, S-C., Poh, C., and Wang, W.: (Carboxymethyl)chitosan-modified superparamagnetic iron oxide nanoparticles for magnetic resonance imaging of stem cells. ACS Appl. Mater. Interfaces 1(2), 328 (2008).
16.Gupta, A.K. and Wells, S.: Surface-modified superparamagnetic nanoparticles for drug delivery: Preparation, characterization, and cytotoxicity studies. IEEE Trans. Nanobiosci. 3(1), 66 (2004).
17.Gupta, A.K. and Gupta, M.: Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26(18), 3995 (2005).
18.da Costa, G.M., De Grave, E., de Bakker, P.M.A., and Vandenberghe, R.E.: Synthesis and characterization of some iron oxides by sol-gel method. J. Solid State Chem. 113(2), 405 (1994).
19.Dave, S.R. and Gao, X.: Monodisperse magnetic nanoparticles for biodetection, imaging, and drug delivery: A versatile and evolving technology. Nanomed. Nanobiotechnol. 1(6), 583 (2009).
20.Teng, X. and Yang, H.: Synthesis of face-centered tetragonal FePt nanoparticles and granular films from Pt@Fe2O3 core-shell nanoparticles. J. Am. Chem. Soc. 125(47), 14559 (2003).
21.Ge, J., Hu, Y., Biasini, M., Beyermann, W.P., and Yin, Y.: Superparamagnetic magnetite colloidal nanocrystal clusters. Angew. Chem. Int. Ed. 46(23), 4342 (2007).
22.Wang, L., Bao, J., Wang, L., Zhang, F., and Li, Y.: One-pot synthesis and bioapplication of amine-functionalized magnetite nanoparticles and hollow nanospheres. Chemistry 12(24), 6341 (2006).
23.Tang, B., Wang, G., Zhuo, L., Ge, J., and Cui, L.: Facile route to α-FeOOH and α-Fe2O3 nanorods and magnetic property of γ-Fe2O3 nanorods. Inorg. Chem. 45(13), 5196 (2006).
24.Taniguchi, T., Nakagawa, K., Watanabe, T., Matsushita, N., and Yoshimura, M.: Hydrothermal growth of fatty acid stabilized iron oxide nanocrystals. J. Phys. Chem. C 113(3), 839 (2008).
25.Deng, H., Li, X., Peng, Q., Wang, X., Chen, J., and Li, Y.: Monodisperse magnetic single-crystal ferrite microspheres. Angew. Chem. Int. Ed. 117(18), 2842 (2005).
26.Ge, J., Hu, Y., Zhang, T., and Yin, Y.: Superparamagnetic composite colloids with anisotropic structures. J. Am. Chem. Soc. 129(29), 8974 (2007).
27.Yuan, Q., Venkatasubramanian, R., Hein, S., and Misra, R.D.K.: A stimulus-responsive magnetic nanoparticle drug carrier: Magnetite encapsulated by chitosan-grafted-copolymer. Acta Biomater. 4(4), 1024 (2008).
28.Wang, X., Zhou, L., Ma, Y., Li, X., and Gu, H.: Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection. Nano Res. 2(5), 365 (2009).
29.Li, G-y., Huang, K-l., Jiang, Y-r., Ding, P., and Yang, D-l.: Preparation and characterization of carboxyl functionalization of chitosan derivative magnetic nanoparticles. Biochem. Eng. J. 40(3), 408 (2008).
30.Mikhaylova, M., Kim, D.K., Berry, C.C., Zagorodni, A., Toprak, M., Curtis, A.S.G., and Muhammed, M.: BSA immobilization on amine-functionalized superparamagnetic iron oxide nanoparticles. Chem. Mater. 16(12), 2344 (2004).
31.Tartaj, P., Gonzalez-Carreno, T., Rebolledo, A.F., Bomati-Miguel, O., and Serna, C.J.: Direct aerosol synthesis of carboxy-functionalized iron oxide colloids displaying reversible magnetic behavior. J. Colloid Interface Sci. 309(1), 68 (2007).
32.Yourdkhani, A. and Caruntu, G.: Highly ordered transition metal ferrite nanotube arrays synthesized by template-assisted liquid phase deposition. J. Mater. Chem. 21(20), 7145 (2011).
33.Yu, X., Shan, Y., Du, B., and Chen, K.: One-pot and template-free fabrication of dendritic and octahedral single-crystal magnetites. CrystEngComm. 13, 1525 (2011).
34.Liang, J., Li, L., Luo, M., and Wang, Y.: Fabrication of Fe3O4 octahedra by a triethanolamine-assisted hydrothermal process. Cryst. Res. Technol. 46(1), 95 (2011).
35.Wang, X., Zhao, Z., Qu, J., Wang, Z., and Qiu, J.: Shape-control and characterization of magnetite prepared via a one-step solvothermal route. Cryst. Growth Des. 10, 2863 (2010).
36.Cheng, W., Tang, K., and Sheng, J.: Highly water-soluble superparamagnetic ferrite colloidal spheres with tunable composition and size. Chem. Eur. J. 16(12), 3608 (2010).
37.Sun, H., Wei, H., Zhang, H., Ning, Y., Tang, Y., Zhai, F., and Yang, B.: Self-assembly of CdTe nanoparticles into dendrite structure: A microsensor to Hg2+. Langmuir 27(3), 1136 (2011).
38.Li, Y., Dong, C., Chu, J., Qi, J., and Li, X.: Surface molecular imprinting onto fluorescein-coated magnetic nanoparticles via reversible addition fragmentation chain transfer polymerization: A facile three-in-one system for recognition and separation of endocrine disrupting chemicals. Nanoscale 3, 280 (2011).
39.Zhou, W.H., Lu, C.H., Guo, X.C., Chen, F.R., Yang, H.H., and Wang, X.R.: Mussel-inspired molecularly imprinted polymer coating superparamagnetic nanoparticles for protein recognition. J. Mater. Chem. 20(5), 880 (2009).
40.Song, X., Yang, Y., Liu, J., and Zhao, H.: PS colloidal particles stabilized by graphene oxide. Langmuir 27(3), 1186 (2011).
41.Wang, C., Tao, S., Wei, W., Meng, C., Liu, F., and Han, M.: Multifunctional mesoporous material for detection, adsorption and removal of Hg2+ in aqueous solution. J. Mater. Chem. 20(22), 4635 (2010).
42.Chen, H., Zhao, Y., Yang, M., He, J., Chu, P.K., Zhang, J., and Wu, S.: Glycine-assisted hydrothermal synthesis of peculiar porous alpha-Fe2O3 nanospheres with excellent gas-sensing properties. Anal. Chim. Acta 659(1–2), 266 (2010).
43.Zhou, K., Zhu, Y., Yang, X., and Li, C.: One-pot preparation of graphene/Fe3O4 composites by a solvothermal reaction. N. J. Chem. 34(12), 2950 (2010).
44.Tomalia, D.A., Baker, H., Dewald, J., Hall, M., Kallos, G., Martin, S., Roeck, J., Ryder, J., and Smith, P.: A new class of polymers: Starburst-dendritic macromolecules. Polym. J. 17(1), 117 (1985).
45.Tomalia, D.A., Baker, H., Dewald, J., Hall, M., Kallos, G., Martin, S., Roeck, J., Ryder, J., and Smith, P.: Dendritic macromolecules: Synthesis of starburst dendrimers. Macromolecules 19(9), 2466 (1986).
46.Zhang, Z., Cui, Y., and Wan, Q.: Surface modification of magnetic silica microspheres and its application to the isolation of plant genomic nucleic acids. Chin. J. Anal. Chem. 35(1), 31 (2007).
47.Roca, A.G., Marco, J.F., Morales, M.P., and Serna, C.J.: Effect of nature and particle size on properties of uniform magnetite and maghemite nanoparticles. J. Phys. Chem. C 111(50), 18577 (2007).
48.Wan, S., Huang, J., Yan, H., and Liu, K.: Size-controlled preparation of magnetite nanoparticles in the presence of graft copolymers. J. Mater. Chem. 16(3), 298 (2006).
49.Hoch, L.B., Mack, E.J., Hydutsky, B.W., Hershman, J.M., Skluzacek, J.M., and Mallouk, T.E.: Carbothermal synthesis of carbon-supported nanoscale zero-valent iron particles for the remediation of hexavalent chromium. Environ. Sci. Technol. 42(7), 2600 (2008).
50.Zhi, J., Wang, Y., Lu, Y., Ma, J., and Luo, G.: In situ preparation of magnetic chitosan/Fe3O4 composite nanoparticles in tiny pools of water-in-oil microemulsion. React. Funct. Polym. 66(12), 1552 (2006).
51.Wang, S.H., Shi, X., Van Antwerp, M., Cao, Z., Swanson, S.D., Bi, X., and Baker, J.R. Jr.: Dendrimer-functionalized iron oxide nanoparticles for specific targeting and imaging of cancer cells. Adv. Funct. Mater. 17(16), 3043 (2007).
52.Narain, R., Gonzales, M., Hoffman, A.S., Stayton, P.S., and Krishnan, K.M.: Synthesis of monodisperse biotinylated p (NIPAAm)-coated iron oxide magnetic nanoparticles and their bioconjugation to streptavidin. Langmuir 23(11), 6299 (2007).
53.McBain, S., Yiu, H., El Haj, A., and Dobson, J.: Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection. J. Mater. Chem. 17(24), 2561 (2007).
54.Taylor, J.I., Hurst, C.D., Davies, M.J., Sachsinger, N., and Bruce, I.J.: Application of magnetite and silica-magnetite composites to the isolation of genomic DNA. J. Chromatogr. A 890(1), 159 (2000).
55.Yoza, B., Matsumoto, M., and Matsunaga, T.: DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound. J. Biotechnol. 94(3), 217 (2002).

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One-pot solvothermal method to prepare functionalized Fe3O4 nanoparticles for bioseparation

  • Guoxin Zhang (a1), Fengxiang Qie (a2), Jianxuan Hou (a2), Shizhong Luo (a2), Liang Luo (a3), Xiaoming Sun (a3) and Tianwei Tan (a4)...

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