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Molecular weight of polyethylenimine-dependent transfusion and selective antimicrobial activity of functional silver nanoparticles

Published online by Cambridge University Press:  20 July 2020

Atul Kumar Tiwari ,
Munesh Kumar Gupta ,
Govind Pandey ,
Roger J. Narayan  and
Prem C. Pandey Open the ORCID record for Prem C. Pandey [Opens in a new window]
Atul Kumar Tiwari
Affiliation:
Department of Chemistry, Indian Institute of Technology, Varanasi, Uttar Pradesh221005, India
Munesh Kumar Gupta
Affiliation:
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh221005, India
Govind Pandey
Affiliation:
Department of Pediatrics, King George Medical University, Lucknow, Uttar Pradesh226003, India
Roger J. Narayan*
Affiliation:
Department of Biomedical Engineering, North Carolina State University, North Carolina27695, USA
Prem C. Pandey*
Affiliation:
Department of Chemistry, Indian Institute of Technology, Varanasi, Uttar Pradesh221005, India
*
a)Address all correspondence to this authors. e-mail: rjnaraya@ncsu.edu and pcpandey.apc@iitbhu.ac.in
a)Address all correspondence to this authors. e-mail: rjnaraya@ncsu.edu and pcpandey.apc@iitbhu.ac.in
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Abstract

Synthetic cationic polymer-mediated synthesis of silver nanoparticles and selective antimicrobial activity of the same were demonstrated. Polyethyleneimine (PEI)-coated silver nanoparticles showed antimicrobial activity against Acinetobacter baumannii as a function of the polymeric molecular weight (MW) of PEI. Silver nanoparticles were coated with PEI of three different MWs: Ag-NP-1 with PEI exhibiting a MW of 750,000, Ag-NP-2 with PEI exhibiting a MW of 1300, and Ag-NP-3 with PEI exhibiting a MW of 60,000. These nanoparticles showed a particle size distribution of 4–20 nm. The nanoparticles exhibited potent antimicrobial activity against A. baumannii, with the minimum inhibitory concentration of Ag-NP-1, Ag-NP-2, and Ag-NP-3 on the order of 5, 10, and 5 μg/mL, respectively, and minimum bactericidal concentration of Ag-NP-1, Ag-NP-2, and Ag-NP-3 on the order of 10, 20, and 10 μg/mL, respectively. Fluorescence imaging of Ag-NPs revealed selective transfusion of Ag-NPs across the cell membrane as a function of the polymeric MW; differential interaction of the cytoplasmic proteins during antimicrobial activity was observed.

Keywords

antimicrobial agentssilver nanoparticlesminimum inhibitory concentrationminimum bactericidal concentration
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 18 , 28 September 2020 , pp. 2405 - 2415
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Copyright
Copyright © Materials Research Society 2020

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